Crops ›› 2026, Vol. 42 ›› Issue (1): 15-19.doi: 10.16035/j.issn.1001-7283.2026.01.003

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Identification of Disease Resistant Genes in Xinjiang Spring Wheat Materials Based on KASP Technology

Jia Yonghong(), Wei Haipeng, Zeng Chaowu, Liu Jun, Chen Yanni, Li Jianjiang(), Liang Xiaodong()   

  1. Institute of Grain Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, Xinjiang, China
  • Received:2024-10-09 Revised:2024-12-03 Online:2026-02-15 Published:2026-02-10

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Abstract:

To identify the distribution of disease resistance functional genes in Xinjiang spring wheat materials and provide materials for genetic improvement of wheat disease resistance breeding, KASP technology was employed to detect genes associated with resistance to stripe rust, leaf rust, powdery mildew, and Fusarium head blight in 549 Xinjiang spring wheat materials. The results showed that 121 materials carried five stripe rust resistance alleles with relatively high frequencies (QYrqin.nwafu-2AL, Yr29, QYrsn.nwafu-1BL, QYrqin.nwafu- 6BS, Yr78); 352 materials carried two leaf rust resistance alleles with relatively high frequencies (Lr67, Lr46); and 142 materials carried three Fusarium head blight resistance alleles with relatively high frequencies (QFhb.Hbaas-5AS, QFhb.hbaas-5AL, QFhb.caas-5AL). No presence of the five powdery mildew resistance genes (Pm12, Pm21, Pm2a, Pm5e, PmV) was detected in any of the materials. In summary, Xinjiang spring wheat varieties exhibited high frequencies of resistance genes for stripe rust, leaf rust, and Fusarium head blight, but a notable lack of resistance sources for powdery mildew. Therefore, it is crucial to focus on introducing and utilizing new powdery mildew resistant materials to improve the disease resistance breeding capacity of Xinjiang spring wheat.

Key words: Wheat, Disease resistance, KASP, Marker detection

Table 1

KASP detection results of stripe rust resistance genes"

基因
Gene		 感病Susceptible 抗病Disease-resistant 杂合型Heterozygous 缺失值NA 未知功能Unknown function
数量
Number		 频率
Frequency
(%)		 数量
Number		 频率
Frequency
(%)		 数量
Number		 频率
Frequency
(%)		 数量
Number		 频率
Frequency
(%)		 数量
Number		 频率
Frequency
(%)
Yr5 421 76.68 95 17.30 33 6.01 0 0.00 0 0.00
Yr26 404 73.59 142 25.87 3 0.55 0 0.00 0 0.00
Yr29 2 0.36 547 99.64 0 0.00 0 0.00 0 0.00
Yr30 100 18.21 252 45.90 5 0.91 192 34.97 0 0.00
Yr75 312 56.83 230 41.89 7 1.28 0 0.00 0 0.00
Yr78 246 44.81 302 55.01 1 0.18 0 0.00 0 0.00
Yr80 275 50.09 270 49.18 4 0.73 0 0.00 0 0.00
Yr82 468 85.25 74 13.48 7 1.28 0 0.00 0 0.00
YrZH58 362 65.94 183 33.33 4 0.73 0 0.00 0 0.00
YrSP 516 93.99 0 0.00 0 0.00 33 6.01 0 0.00
QYrsn.nwafu-1BL 134 24.41 373 67.94 7 1.28 35 6.38 0 0.00
QYrxn.nwafu-1BL 344 62.66 203 36.98 2 0.36 0 0.00 0 0.00
QYrsn.nwafu-2AS 374 68.12 32 5.83 4 0.73 139 25.32 0 0.00
QYrqin.nwafu-2AL 1 0.18 548 99.82 0 0.00 0 0.00 0 0.00
QYrhm.nwafu-2BC 40 7.29 140 25.50 2 0.36 1 0.18 0 0.00
QYrqin.nwafu-2BL 429 78.14 86 15.66 31 5.65 3 0.55 0 0.00
QYr.nwafu-3BS 7 1.28 166 30.24 375 68.31 1 0.18 0 0.00
QYrsn.nwafu-3DL 409 74.50 133 24.23 5 0.91 0 0.00 0 0.00
QYr.nwafu-4BL 494 89.98 52 9.47 3 0.55 0 0.00 0 0.00
QYrsn.nwafu-6BS 482 87.80 47 8.56 16 2.91 4 0.73 0 0.00
QYrqin.nwafu-6BS 182 33.15 362 65.94 5 0.91 0 0.00 0 0.00

Table 2

KASP detection results of leaf rust resistance genes"

基因
Gene		 感病Susceptible 抗病Disease-resistant 杂合型Heterozygous 缺失值NA 未知功能Unknown function
数量
Number		 频率
Frequency
(%)		 数量
Number		 频率
Frequency
(%)		 数量
Number		 频率
Frequency
(%)		 数量
Number		 频率
Frequency
(%)		 数量
Number		 频率
Frequency
(%)
Lr13 254 46.27 37 6.74 5 0.91 253 46.08 0 0.00
Lr21 548 99.82 0 0.00 0 0.00 1 0.18 0 0.00
Lr37 395 71.95 151 27.50 2 0.36 1 0.18 0 0.00
Lr46 195 35.52 352 64.12 2 0.36 0 0.00 0 0.00
Lr67 0 0.00 548 99.82 0 0.00 1 0.18 0 0.00
Lr68 458 83.42 87 15.85 3 0.55 1 0.18 0 0.00
Lr80 547 99.64 0 0.00 0 0.00 2 0.36 0 0.00

Table 3

KASP detection results of powdery mildew resistance genes"

基因
Gene		 感病Susceptible 抗病Disease-resistant 杂合型Heterozygous 缺失值NA 未知功能Unknown function
数量
Number		 频率
Frequency
(%)		 数量
Number		 频率
Frequency
(%)		 数量
Number		 频率
Frequency
(%)		 数量
Number		 频率
Frequency
(%)		 数量
Number		 频率
Frequency
(%)
Pm12 543 98.91 0 0.00 0 0.00 6 1.09 0 0.00
Pm21 548 99.82 0 0.00 1 0.18 0 0.00 0 0.00
Pm2a 549 100.00 0 0.00 0 0.00 0 0.00 0 0.00
Pm5e 466 84.88 0 0.00 0 0.00 83 15.12 0 0.00
PmV 544 99.09 0 0.00 0 0.00 5 0.91 0 0.00

Table 4

KASP detection results of Fusarium head blight resistance genes"

基因
Gene		 感病Susceptible 抗病Disease-resistant 杂合型Heterozygous 缺失值NA 未知功能Unknown function
数量
Number		 频率
Frequency
(%)		 数量
Number		 频率
Frequency
(%)		 数量
Number		 频率
Frequency
(%)		 数量
Number		 频率
Frequency
(%)		 数量
Number		 频率
Frequency
(%)
QFhb.caas-3BL
 508 92.53 40 7.29 0 0.00 1 0.18 0 0.00
QFhb.caas-5AL 268 48.82 275 50.09 5 0.91 0 0.00 0 0.00
QFhb.hbaas-1AS
 432 78.69 0 0.00 2 0.36 0 0.00 115 20.95
QFhb.hbaas-5AS 115 20.95 432 78.69 2 0.36 0 0.00 0 0.00
QFhb.hbaas-5AL 135 24.59 411 74.86 3 0.55 0 0.00 0 0.00
Fhb1-His 0 0.00 0 0.00 0 0.00 1 0.18 548 99.82
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