Crops ›› 2025, Vol. 41 ›› Issue (5): 128-134.doi: 10.16035/j.issn.1001-7283.2025.05.017

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KASP Marker Detection and Analysis of Wheat Variety Luomai 906 and Its Derivative Lines

Li Tianqi(), Cao Yanyan(), Ge Changbin(), Li Leilei, Huang Jie, Liao Pingʼan   

  1. Luohe Academy of Agricultural Sciences, Luohe 462300, Henan, China
  • Received:2025-03-13 Revised:2025-05-02 Online:2025-10-15 Published:2025-10-21

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Abstract:

To investigate the functional gene composition of important traits in wheat variety Luomai 906 and its 12 derived lines, this study utilized high-throughput KASP marker technology to detect genes associated with adaptability, quality, yield, stress resistance, and disease resistance in 13 tested materials. The results showed that 13 materials carried two dwarfing genes Rht-D1b and Rht8. All 13 materials contained the superior allelic variant TaPRR73-B1 related to maturity period. Nine materials shared the superior photoperiod-related allelic variants Ppd-D1a and Ppd-B1a. Ten materials carried two yield-related genes, while Luomai 86 carried three superior yield-related allelic variants. Ten materials carried the superior grain hardness gene Pinb-D1b, and three materials contained Pina-D1b/Null. All 13 materials carried the high-quality subunits Ax1/Ax2*, while two materials contained the premium subunits 5+10. Notably, Luomai 906 and its derived line Luomai 59 both carried two high-quality subunits. One material contained two drought resistance genes, all 13 materials carried two pre-harvest sprouting resistance genes, and five materials carried the stripe rust resistance gene Yr78. Additionally, Luomai 906 was found to carry 17 superior allelic genes, genetic analysis of its 12 derived lines using these 17 loci revealed that 14 KASP marker loci exhibited a genetic contribution rate exceeding 75%, with six loci reaching 100%. This study clarifies the superior allelic variations in Luomai 906 and its derived lines, as well as their genetic contributions in progeny, providing critical theoretical support for research and utilization of Luomai 906 as a key parental line, and the breeding of Luomai series wheat varieties.

Key words: Wheat, Luomai 906, Important parent, Derivative lines, KASP assay

Table 1

Information of Luomai 906 and its derivative lines"

品种（系）Cultivar (line) 系谱Pedigree 世代Generation 参试情况Test result
漯麦906 Luomai 906 周麦18/野二二燕 小麦亲本 2019年豫审
漯麦55 Luomai 55 周98165/漯麦906 衍生一代 2025年国审
漯麦58 Luomai 58 漯麦6010/提葡//漯麦906 衍生一代 生产试验
漯麦59 Luomai 59 漯麦906/济麦22 衍生一代 2025年豫审
漯麦61 Luomai 61 中麦895/漯麦906 衍生一代 生产试验
漯麦62 Luomai 62 周麦22/漯麦906 衍生一代 生产试验
漯麦80 Luomai 80 周麦22/漯麦906 衍生一代 生产试验
漯麦81 Luomai 81 周麦23/漯麦906 衍生一代 产比
漯麦86 Luomai 86 漯麦906/郑麦1860 衍生一代 品比
漯麦87 Luomai 87 漯麦906/郑麦7698 衍生一代 产比
漯麦88 Luomai 88 漯麦906/漯麦163 衍生一代 区试
漯麦91 Luomai 91 漯麦906/漯麦6010 衍生一代 产比
漯麦92 Luomai 92 漯麦906/郑麦7698 衍生一代 区试

Table 2

Genes and corresponding gene markers"

基因
Gene		 基因标记
Gene marker		 基因
Gene		 基因标记
Gene marker
Rht-B1 Rht-B1_SNP NAM-6A NAM-6A_SNP1
Rht-D1 Rht-D1_SNP Psy-D1 Psy1Da-g
Rht8 Rht8 Zds-A1 ZDS-A1_SNP
TaPRR73-A1 PRR73A1-9IND TaPds-B1 PDS-B1_SNP
TaPRR73-B1 PRR73B1-4558 Pod-A1 Pod-A1
Ppd-A1 GS105-1117_InDel Lox-B1 Lox-B1
Ppd-B1 TaPpdBJ001 SST-4D SST-4D-1093
Ppd-D1 TaPpdDJ001 TaDreb-B1 TaDreb_SNP
Sus1-7A Sus1-7A-1185_SNP 1-fehw3 1-FEH-6B
TaGASR7-A1 TaGASR TaPHS1 PHS1-646
TaSus2-2A Sus2-2A-20SNP Sdr-B1 Sdr-B1
TaGS5 GS5_SNP COMT-3B COMT3B_882_SNP
TEF-7A TEF7A-1-bp_IND Pm21 Pm21_SNP
Pina-D1 Pina-D1 Fhb1 Fhb1_KSU
Pinb-D1 Pinb-D1 Yr78 IWA7257
Pinb2-V Pinb2-Bv2 Lr46 Lr46_JF2-2A
Glu-D1 Glu-D1_SNP Lr16 kwm847
Glu-A1 Glu-A1-13 Lr48 IWB70147
Gpc GPC_DUC

Table 3

KASP detection results of Luomai 906 and its derivative lines"

基因
Gene		 等位变异
Variation		 表型
Phenotype		 漯麦906
Luomai
906		 漯麦55
Luomai
55		 漯麦58
Luomai
58		 漯麦59
Luomai
59		 漯麦61
Luomai
61		 漯麦62
Luomai
62		 漯麦80
Luomai
80		 漯麦81
Luomai
81		 漯麦86
Luomai
86		 漯麦87
Luomai
87		 漯麦88
Luomai
88		 漯麦91
Luomai
91		 漯麦92
Luomai
92
Rht-B1 Rht-B1a 野生型 + + + + + + + + + + + ns +
Rht-B1b 矮秆型
Rht-D1 Rht-D1a 野生型
Rht-D1b 矮秆型 + + + + + + + + + + + + +
Rht8 Rht8+ 含矮秆基因 + + + + + + + + + + + + +
Rht8- 不含矮秆基因
TaPRR73-A1 Hap-I 晚熟，矮
Hap-II 早熟，高 + + + + + + + + + + + + +
TaPRR73-B1 Hap-I 早熟，高 +
Hap-II 晚熟，矮 + + + + + + + + + + + +
Ppd-A1 Ppd-A1a 光周期迟钝 ns
Ppd-A1b 光周期敏感 + + + + + + + + + + + +
Ppd-B1 Ppd-B1a 光周期迟钝 + + + + + + + + ns +
Ppd-B1b 光周期敏感 + + +
Ppd-D1 Ppd-D1a 光周期迟钝 + + + + + + + + + + + ns +
Ppd-D1b 光周期敏感
Sus1-7A Hap-I 高千粒重
Hap-II 低千粒重 + + + + + + + + + + + + +
TaGASR7-A1 H1C 高千粒重 + ns ns
H1G 低千粒重 + + + + + + + + + +
TaSus2-2A Hap-A 高千粒重 ns
Hap-G 低千粒重 + + + + + + + + + + + +
TaGS5 TaGS5-Ala 大粒，粒重高 + + + + + ns ns + + + + + +
TaGS5-Alb 小粒，粒重低
TEF-7A Hap-7A-3 粒数较多 + + ns + + + + + + + + + +
Hap-7A-1/2 粒数较少
Pina-D1 Pina-D1a 软质 + + + + + + + + + +
Pina-D1b/Null 硬质 + + +
Pinb-D1 Pinb-D1a 软质 ns + +
Pinb-D1b 硬质 + + + + + + + + + +
Pinb2-V Pinb-B2a 软质 + + + + + + + + + + + + +
Pinb-B2b 硬质
Glu-D1 2+12 弱筋 + ns + + + + + + + + +
5+10 强筋 + +
Glu-A1 AxNull 弱筋
Ax1/Ax2* 强筋 + + + + + + + + + + + + +
Gpc Gpc-B1- 籽粒蛋白质正常 + + + + + + + + + + + +
Gpc-B1+ 籽粒蛋白质增加 +
NAM-6A A1c/A1d 籽粒蛋白质正常 + + + + + + + + + +
A1a/A1b 籽粒蛋白质增加 + + +
Psy-D1 Psy-D1a 黄色素含量低 ns
Psy-D1g 黄色素含量高 + + + + + + + + + + + +
Zds-A1 Zds-A1a 黄色素含量高 + + + + + ns + + + + + +
Zds-A1b 黄色素含量低 +
TaPds-B1 TaPds-B1a 黄色素含量高 + + + + + + + + + + + +
TaPds-B1b 黄色素含量低 +
Pod-A1 TaPod-A1a 过氧化物酶活性低 + ns + + + + + ns + + ns
TaPod-A1b 过氧化物酶活性高 + +
Lox-B1 Lox-B1b 脂肪氧化酶活性低 ns
Lox-B1a 脂肪氧化酶活性高 + + + + + + + + + + + +
SST-4D TaSST-D1a 可溶性糖含量高 + + ns + ns + ns
TaSST-D1b 可溶性糖含量低 + + + + + +
TaDreb-B1 TaDreb-B1a 抗旱 + + + ns +
TaDreb-B1b 不抗旱 + + + + + + + +
1-fehw3 Kauz 不抗旱 + + ns + + +
Westonia 抗旱 + + + + + + +
TaPHS1 NW97S186 感穗发芽
Rio Blanco 抗穗发芽 + + + + + + + + + + + + +
Sdr-B1 TaSdr-B1b 感穗发芽
TaSdr-B1a 抗穗发芽 + + + + + + + + + + + + +
COMT-3B COMT-3Ba 木质素含量高 ns + + + + + + +
COMT-3Bb 木质素含量低 + + + + +
Pm21 Pm21- 感白粉病 + + + + + + + + + + + + +
Pm21+ 抗白粉病
Fhb1 Fhb1- 感赤霉病 + + + + + + + + + + + + +
Fhb1+ 抗赤霉病
Yr78 Yr78- 感条锈病 ns + + + + + + +
Yr78+ 抗条锈病 + + + + +
Lr46 Lr46- 感叶锈病 + + + + + + + + + + + + +
Lr46+ 抗叶锈病
Lr16 Lr16- 感叶锈病 + + + + + + + + + + + + +
Lr16+ 抗叶锈病
Lr48 Lr48- 感叶锈病 + + + + + + + + + + + + +
Lr48+ 抗叶锈病

Fig.1

Proportion of allelic variation detection of test materials"

Table 4

Detection results of Luomai 906 excellent loci in derived offspring"

基因
Gene		 标记
Maker		 衍生系优异
等位基因个数
Number of
excellent alleles		 遗传贡献率
Contribution
ratio (%)
Rht-D1 Rht-D1_SNP 12 100.00
Rht8 Rht8 12 100.00
TaPRR73-B1 PRR73B1-4558 12 100.00
Ppd-D1 TaPpdDJ001 11 91.67
TaGS5 GS5_SNP 10 83.33
TEF-7A TEF7A-1-bp_IND 11 91.67
Pinb-D1 Pinb-D1 9 75.00
Glu-D1 Glu-D1_SNP 1 8.33
Glu-A1 Glu-A1-13 12 100.00
NAM-6A NAM-6A_SNP1 2 16.67
Psy-D1 Psy1Da-g 11 91.67
Zds-A1 ZDS-A1_SNP 10 83.33
TaPds-B1 PDS-B1_SNP 11 91.67
SST-4D SST-4D-1093 3 25.00
TaPHS1 PHS1-646 12 100.00
Sdr-B1 Sdr-B1 12 100.00
Lox-B1 Lox-B1 11 91.67
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