Crops ›› 2016, Vol. 32 ›› Issue (6): 53-57.doi: 10.16035/j.issn.1001-7283.2016.06.010

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Effects of Low Temperature Stress on Lipid Peroxidation and Osmotic Regulation Substances of Chewing Cane Seedlings

Chen Minghui1,Zhang Zhilu1,Cheng Shiping1,Huang Xing2,Zhang Baoqing2   

  1. 1 The Key Laboratory of Ecological Restoration in Low Mountain and Hilly Areas,Pingdingshan University,Pingdingshan 467000,Henan,China
    2 Sugarcane Research Center,Chinese Academy of Agricultural Sciences/Sugarcane Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi,China
  • Received:2016-08-24 Revised:2016-09-26 Online:2016-12-15 Published:2018-08-26

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Abstract:

With seedling leaves of four introduced chewing cane cultivars as the experimental materials, the relative conductivity, malondialdehyde (MDA) content, soluble sugar content, soluble protein content, proline content were investigated to determinate the effect of low temperature stress. The results showed that with the prolongation of treatment time, the relative electric conductivity (REC) of four cultivars seedlings kept an overall rising trend and MDA content showed a trend of first significant increase and then decreasing. REC and MDA content reached the max value under low temperature stress in 7 and 10 days, respectively, with the order of CZ26>Badila>Minyin huangpi sugarcane>Guiguozhe 1.With the prolonged treatment, contents of soluble sugar and protein as well as proline increased.The content of soluble sugar and protein were significantly increased at all time. And proline content sharply increased in the earlier stage and slowly decreased again with the extension of stress. However, the content of proline remained at a relatively high level throughout the stress of low temperature. The ability of cold resistance on the four introduced chewing cane cultivars from high to low was Guiguozhe 1>Minyin huangpi sugarcane>Badila>CZ26.

Key words: Low temperature stress, Chewing cane, Lipid peroxidation, Osmotic regulation substances

Fig.1

Effects of low temperature on relative electric conductivity of four chewing cane cultivars seedlings Different small letters mean significant difference at 0.05 level,the same below"

Fig.2

Effects of low temperature on MDA content of four chewing cane cultivars seedlings"

Fig.3

Effects of low temperature on soluble sugar content of four chewing cane cultivars seedlings"

Fig.4

Effects of low temperature on soluble protein content of four chewing cane cultivars seedlings"

Fig.5

Effects of low temperature on proline content of four chewing cane cultivars seedlings"

Table 1

Variation coefficient of indexes of different treatment time at low temperature %"

指标Index 变异系数Coefficient of variation
0d 1d 3d 7d 10d 14d
细胞膜透性含量
Cytomembrane permeability		 2.05
 5.67
 5.57
 6.79
 11.47
 13.15
丙二醛含量MDA content 2.59 5.05 5.23 7.20 5.20 5.52
可溶性糖Soluble sugar content 4.76 12.59 6.56 8.51 13.94 14.75
可溶性蛋白质含量
Soluble protein content		 5.92
 8.86
 14.24
 7.46
 14.42
 15.88
脯氨酸含量Proline content 6.43 8.85 14.43 18.97 20.46 15.81
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