Crops ›› 2016, Vol. 32 ›› Issue (5): 87-93.doi: 10.16035/j.issn.1001-7283.2016.05.015

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Effects of Exogenous ALA on Growth and Photosynthetic Characteristics of Maize Seedlings under Low Temperature Stress

Sun Yang1,Wang Yi1,Meng Yao2,Fan Haichao1,Qu Danyang1,Li Jing1,Wei Shi1,Gu Wanrong1   

  1. 1 College of Agronomy,Northeast Agricultural University,Harbin 150030,Heilongjiang,China
    2 Heilongjiang Land Reclamation Academy of Sciences,Harbin 150038,Heilongjiang,China
  • Received:2016-07-15 Revised:2016-08-18 Online:2016-10-15 Published:2018-08-26
  • Contact: Wanrong Gu

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Abstract:

The objective of this study was to investigate the seedling growth and photosynthetic characteristics of exogenous ALA-enhanced maize seedlings under low temperature stress. This experiment was conducted with maize cultivars Suiyu 13 (cold sensitivity) and Zhengdan 958 (cold resistance), which grew up to three-leaf, then were treated with low temperature of 14℃/5℃(day/night) for 48h, and then got recovery for 48h. Plant morphological growth, chlorophyll content, photosynthetic parameters, chlorophyll fluorescence characteristics were measured. The results showed that seedling height, leaf area, fresh weight and dry weight decreased significantly under low temperature stress. All the decreases in SPAD value, Pn, Tr, Gs, Ci, Fm, Fv/Fm, Fv/Fo, qP and the increases in Fo and NPQ reflected the inhibitory effects of low temperature stress. Exogenous ALA could promote the matter accumulation and morphogenesis, increase SPAD value of maize seedlings under low temperature stress. Exogenous ALA application led to a marked increase in Pn, Tr, Gs, Ci, Fm, Fv/Fm, Fv/Fo and qP, and reduce Fo and NPQ of two maize varieties. Therefore, spraying exogenous ALA could promote the photosynthesis , PSⅡ reactivity and matter accumulation, as well as enhanceed the low temperature resistance of maize seedlings. The alleviation effect of exogenous ALA was better in cold sensitivity type under low temperature stress.

Key words: 5-aminolevulinic acid, Low temperature stress, Seedling growth, Photosynthetic characteristics

Table 1

Effects of ALA on seedling height,leaf area,fresh weight and dry weight under low temperature stress"

品种Variety 处理Treatment 株高Plant height(cm) 叶面积Leaf area(cm2/株) 鲜重Fresh weight(g/株) 干重Dry weight(g/株)
绥玉13 CK 18.83±0.76a 72.77±1.97a 1.507±0.03a 0.130±0.01a
Suiyu 13 L 14.83±0.76b 54.46±4.43b 1.076±0.13b 0.106±0.01b
L+ALA 19.67±0.29a 70.98±0.57a 1.458±0.06a 0.139±0.01a
郑单958 CK 25.37±2.14a 74.56±3.43a 1.592±0.08a 0.160±0.03a
Zhengdan 958 L 19.87±2.11b 64.19±3.50b 1.217±0.20b 0.108±0.01b
L+ALA 24.57±1.10a 72.85±5.16a 1.581±0.10a 0.137±0.01ab

Fig.1

Effects of ALA on chlorophyll content of maize seeding leaves under low temperature stress Different letters mean significant difference at 0.05 level,the same below"

Fig.2

Effects of ALA on photosynthetic rate of maize seeding leaves under low temperature stress"

Fig.3

Effects of ALA on transpiration rate of maize seeding leaves under low temperature stress"

Fig.4

Effects of ALA on stomatal conductance of maize seeding leaves under low temperature stress"

Fig.5

Effects of ALA on Ci of maize seeding leaves under low temperature stress"

Fig.6

Effects of ALA on Fo、Fm、Fv/Fm and Fv/Fo of maize seeding leaves under low temperature stress"

Fig.7

Effects of ALA on qP and NPQ of maize seeding leaves under low temperature stress"

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