Crops ›› 2016, Vol. 32 ›› Issue (6): 49-52.doi: 10.16035/j.issn.1001-7283.2016.06.009

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High Throughput Analysis of the Endophytic Bacterial Community Structure in Roots and Rhizosphere of Highland Barley of Tibetan Plateau

Liu Zhendong1,Wang Bo2,Xue Bei1,Wang Qiangfeng3,Hou Lei1   

  1. 1 Tibet Agricultural and Animal Husbandry College,Nyingchi 860000,Tibet,China
    2 Agricultural Institute,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa 850032,Tibet,China
    3 Institute of Biological & Nuclear Technology,Sichuan Academy of Agricultural Sciences,Chengdu 610061,Sichuan,China;
  • Received:2016-08-27 Revised:2016-10-18 Online:2016-12-15 Published:2018-08-26

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Abstract:

High-throughput sequencing technology has been applied to the determination of 16S V4 variation sequences of the endophytic bacteria in roots and rhizosphere of the main cultivar of highland barley (Zangqing 2000) in Tibetan Plateau. The number of sample sequences and operational taxonomic units (OTUs) were sorted and calculated and the species richness and alpha diversity index were analyzed by Uparse and KRONA. The results showed that the useful sequences and OTUs of the endophytic bacteria in roots were 55 192/3 613, while the useful sequences and OTUs of the endophytic bacteria in rhizosphere were 62 181/4 399. The Chao1 indices of the endophytic bacteria in roots and rhizosphere were 3 443.4290 and 4 632.5820, respectively, and the Shannon diversity indices were 8.542 and 9.967, respectively. The endophytic bacteria in roots and rhizosphere of highland barley were mainly included in Pseudomonas, Streptomyces, Pedobacter, Flavobacterium and Rhodoplanes. These results indicate that the endophytic bacteria in roots and rhizosphere of highland barley ZQ2000 have rich diversity and the dominant genera of the bacteria in both roots and rhizosphere.

Key words: Highland barley, Root, Rhizosphere, Endophytic bacteria, Species, Richness

Table 1

The mumber of tag and OTU of samples"

样品sample Total tag Taxon tag Unclassified Mitochondria Chloroplast tag Unique tag OTU number
ZQ2000.1.r 67 671 54 226 9 896 3 549 3 669
ZQ2000.2.r 63 671 51 708 8 406 3 557 3 588
ZQ2000.3.r 67 264 59 643 4 372 3 249 3 583
平均值Mean 66 202 55 192 7 558 3 452 3 613
ZQ2000.1.s 59 159 53 413 164 5 582 4 009
ZQ2000.2.s 75 030 69 298 87 5 645 4 930
ZQ2000.3.s 69 506 63 832 255 5 419 4 257
平均值Mean 67 898 62 181 169 5 547 4 399

Fig.1

Sequence number at different clacsification level"

Table 2

Relative abundance at phylum level %"

样品Sample Proteobacteria Bacteroidetes Actinobacteria Acidobacteria Firmicutes Others
ZQ2000.1.r 61.1 14.1 13.6 2.9 0.8 21.6
ZQ2000.2.r 63.8 14.6 9.8 3.1 0.8 22.5
ZQ2000.3.r 70.0 11.8 8.6 2.7 0.6 18.1
平均值Mean 64.9 13.5 10.6 2.9 0.7 20.9
ZQ2000.1.s 47.3 11.9 8.8 7.4 0.4 36.1
ZQ2000.2.s 40.0 7.9 8.5 11.9 0.5 39.1
ZQ2000.3.s 52.5 9.7 9.2 7.6 0.7 30.0
平均值Mean 46.6 9.8 8.8 8.9 0.5 35.2

Table 3

Species relative abundance at genus level %"

样品Sample Pseudomonas Streptomyces Pedobacter Flavobacterium Rhodoplanes Others
ZQ2000.1.r 1.7 2.5 0.8 0.6 0.8 93.6
ZQ2000.2.r 1.4 1.5 0.9 0.4 0.8 95.0
ZQ2000.3.r 3.1 1.0 0.8 1.2 0.7 93.2
平均值Mean 2.1 1.7 0.8 0.7 0.8 93.9
ZQ2000.1.s 0.6 0.5 0.5 0.6 1.2 96.6
ZQ2000.2.s 0.4 0.4 0.1 0.1 2.4 96.6
ZQ2000.3.s 0.6 0.5 0.4 0.5 1.8 96.2
平均值Mean 0.5 0.5 0.3 0.4 1.8 96.5

Table 4

Statistic of alpha diversity indices"

样品Sample Shannon Chao 1 物种数Observed species
ZQ2000.1.r 8.0860 3 495.1635 2 997
ZQ2000.2.r 8.7512 3 397.1178 2 933
ZQ2000.3.r 8.0134 3 438.0043 2 888
平均值Mean 8.2835 3 443.4290 2 939
ZQ2000.1.s 9.9022 3 911.0820 3 448
ZQ2000.2.s 10.1277 5 361.9856 4 077
ZQ2000.3.s 9.8716 4 624.6773 3 582
平均值Mean 9.9672 4 632.5820 3 702
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