Crops ›› 2019, Vol. 35 ›› Issue (3): 185-191.doi: 10.16035/j.issn.1001-7283.2019.03.031

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Effects of Alkalized Olive Oil Processing Liquid Wastes on Seed Germination and Seedling Growth of Zea mays L.

Ye Wenbin1,2,3,He Yupeng1,2,Wang Yu1,2,Wang Han1,2,Zhao Qingfang4   

  1. 1 School of Agriculture and Forestry Technology, Longnan Normal College, Chengxian 742500, Gansu, China
    2 Center for Research & Development of Longnan Characteristic Agro-Bioresources, Chengxian 742500, Gansu, China;
    3 Key Laboratory of Gansu University for Rural E-Commerce Talents Training, Chengxian 742500, Gansu, China
    4 College of Life Sciences, Northwest Normal University, Lanzhou 730030, Gansu, China
  • Received:2018-09-30 Revised:2019-04-16 Online:2019-06-15 Published:2019-06-12

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Abstract:

The purpose of this study is to study the effects of waste liquid from olive oil processing on the seed germination and seedling growth of Zea mays L. so as to provide references for the utilization of olive oil waste and farmland environmental protection. Olive oil processing waste liquid (10%-60%) alkalified with 0.10% Na2CO3, was applied to investigate the effects on seed germination, seedling growth and physiological and biochemical characteristics in maize. Alkalinized waste solution (30%) could significantly promote maize seed germination and seedling growth. With the increasing concentration of alkaline waste liquid, the germination rate was significantly reduced and seedling growth was blocked, biomass accumulation was limited, seedling uniformity was also decreased. O2 -· producing rate and H2O2 content in the leaves increased significantly and the accumulation of free proline, soluble sugar and malondialdehyde in the seedlings was obvious. The activities of superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase in the seedlings first increased and then decreased, which showed a trend of high concentration inhibition and low concentration induced enhancement. These results suggested that 30% alkalized waste liquid could effectively promote seed germination and seedling growth of maize. The results of this research provide important reference values for the processing and application of olive oil processing waste liquid.

Key words: Olive oil processing waste liquid, Alkalization, Maize, Seed germination, Seedlings growth, Physiological and biochemical characteristics

Table 1

Main composition and physical and chemical properties of olive oil processing waste liquid treated by Na2CO3 with different concentrations"

指标Index Na2CO3浓度Na2CO3concentration (%)
CK(0) 0.05 0.10 0.20
pH 4.66 5.39 6.96 8.36
导电性Conductivity (mS/cm) 5.56 6.14 6.84 7.65
干物质Dry matter (g/L) 65.23 66.53 66.97 67.13
有机质Organic matter (g/L) 45.36 45.86 46.12 46.81
油脂Oil (g/L) 1.63 1.14 0.93 0.81
多酚Polyphenols (g/L) 1.25 1.04 0.75 0.58
糖类Sugar (g/L) 2.19 2.28 2.39 2.44
有机酸Organic acid (g/L) 0.73 0.64 0.52 0.41
总氮Total nitrogen (g/L) 0.35 0.36 0.45 0.49
Na (g/L) 0.04 0.05 0.06 0.12
K (g/L) 0.76 0.76 0.76 0.76
Mg (g/L) 0.08 0.08 0.08 0.08
Cl (g/L) 0.59 0.59 0.59 0.59

Fig.1

Effects of olive oil processing waste liquid with different concentrations and 0.10% Na2CO3 on seed germination of maize CK(0) is distilled water treatment, CK(0.1) is 0.10% of Na2CO3 treatment, the same below"

Fig.2

Effects of alkalized olive oil processing waste liquid with different concentrations on seed germination of maize K(10)-K(60) indicate that 10% to 60% olive oil processing waste liquor treated with 0.10% Na2CO3, the same below"

Fig.3

Effects of alkalized olive oil processing waste liquid with different concentrations on seedling growth of maize"

Fig.4

Effects of different concentration of alkalized olive oil processing waste liquid on the seedling uniformity of maize"

Fig.5

Effects of alkalized olive oil processing waste liquid with different concentrations on contents of free proline,soluble sugar and MDA of maize seedling"

Fig.6

Effects of alkalized olive oil processing waste liquid with different concentrations on O2-·producing rate and H2O2 content of maize seedling"

Fig.7

Effects of alkalized olive oil processing waste liquid with different concentrations on the activities of SOD, POD, CAT and APX in maize seedling"

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