Crops ›› 2022, Vol. 38 ›› Issue (3): 181-186.doi: 10.16035/j.issn.1001-7283.2022.03.026

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Endogenous Hormones and Enzyme Activity Analysis in Sugarcane Varieties with Different Perennial Root Ages

Cheng Qin(), Tan Qinliang, Li Jiahui, Zhu Pengjin, Zhou Quanguang, Ou Kewei, Lu Yefei, Lü Ping, Pang Xinhua()   

  1. Guangxi Subtropical Crops Research Institute, Nanning 530001, Guangxi, China
  • Received:2021-04-02 Revised:2021-08-30 Online:2022-06-15 Published:2022-06-20
  • Contact: Pang Xinhua E-mail:389208914@qq.com;gxpxh@126.com

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Abstract:

There is a big difference in ratooning ability in different sugarcane varieties. To study the performance of sugarcane varieties in different ratoon years, soluble sugar, soluble protein, endogenous hormone contents and related enzyme activities of roots, stems and leaves at perennial root germination stage were measured using Guire (GR2) and ROC22. The results showed that, the main concentration of soluble sugar and soluble protein were in the stems and leaves and the highest content of soluble sugar was found in the stems of the five-year perennial sugarcane GR2; the largest decline of ABA content was in GR2 which was the most related to ratooning ability, GA3 was negatively correlated with the ratooning ability which was contrary to the trend of ABA and IAA; the plant catalase (CAT) and phenylalanine ammonialyase (PAL) were positively correlated with the ratooning ability, while the sucrose synthase (SS) was negatively correlated with the ratooning ability. The activity of protease was the highest in GR2 of five-year ratooning; the weight coefficients of ABA, IAA, GA3, CAT, PAL, protease, SOD and SS were 0.186, 0.136, 0.107, 0.074, 0.115, 0.165, 0.056, 0.159 by the pole ordering method, respectively. Among them, the weight coefficients of ABA content, protease and SS activities were higher, indicating that the three indexes had a greater impact on the ratooning ability; the ratooning ability were ranked as GF > GT > RT > RF, so GR2 was worth of popularizing because of stronger ratooning ability.

Key words: Sugarcane, Ratooning ability, Endogenous hormone, Enzyme activity, Pole ordering method

Table 1

Agronomic and yield traits of sugarcane ratoon for two and five years"

编号
Number		 株高
Plant height
(cm)		 茎径
Stem diameter
(cm)		 单茎重
Productive
stalk (kg)		 小区有效茎数
Effective stems
per plot		 有效茎数
Effective stem
(×104/hm2)		 小区产量
Grain yield
per plot (kg)		 产量
Yield
(kg/hm2)		 田间锤度
Hammer
degree (%)		 蔗糖分
Sugar
(%)		 含糖量
Sugar content
(kg/hm2)
GT 345aA 2.64aA 1.72aA 312cC 8.68aA 435.6cC 121 020aA 19.83aA 13.77aA 16 605aA
RT 330aA 2.59aA 1.59aA 269cC 7.48bAB 299.8dD 83 280bB 20.63aA 14.63aA 12 210bB
GF 341aA 2.80aA 1.92aA 564aA 8.48aA 814.1aA 122 325aA 19.87aA 13.80aA 16 875aA
RF 321aA 2.80aA 1.80aA 430bB 6.47cB 588.2bB 88 380bB 20.10aA 14.06aA 12 375bB

Fig.1

Soluble sugar and protein contents"

Fig.2

Endogenous hormone contents of root, stem and leaf at sprouting stage Different capital and lowercase letters indicate significant difference at 0.01 and 0.05 level, respectively, the same below"

Table 2

Variance analysis of endogenous hormones for roots"

编号Number ABA (μg/L) IAA (ng/L) GA3 (pg/mL)
RT 212.73±1.89cC 277.89±3.62dD 448.01±2.67aA
GT 352.86±2.50aA 430.54±3.62aA 338.88±4.01cC
RF 287.86±3.00bB 320.42±2.49cC 356.38±2.84bB
GF 210.14±2.75cC 342.44±2.37bB 330.33±1.77cC

Fig.3

The enzyme activities of root, stem and leaf at sprouting stage"

Table 3

The variance analysis of enzyme activity for roots"

编号Number CAT (U/mL) PAL (U/L) Protease (U/L) SOD (U/L) SS (U/mL)
RT 7.40±0.03dD 46.13±0.57aA 471.75±5.57bB 3730.93±377.40bA 11.68±0.15dD
GT 7.77±0.12cC 41.48±0.24bB 347.69±7.61cC 3573.41±41.50bA 18.17±0.22aA
RF 8.03±0.08bB 32.07±0.18cC 328.50±4.08dD 4128.14±41.50aA 12.53±0.04cC
GF 9.31±0.07aA 45.93±0.66aA 524.42±2.04aA 3888.44±57.10abA 17.25±0.29bB

Table 4

Scores of root of two varieties in different measurement indexes during perennial root germination stage (evaluation matrix)"

编号Number ABA IAA GA3 CAT PAL Protease SOD SS
RT 5.28 5.50 1.71 4.33 2.11 2.25 1.38 3.17
GT 3.50 5.50 4.64 2.67 6.00 2.36 4.12 1.93
RF 3.50 1.71 2.87 1.67 4.33 4.33 4.12 3.22
GF 5.50 4.58 3.00 2.07 2.79 4.33 2.82 4.84

Table 5

Comprehensive evaluation of perennial roots at germination stage"

编号
Number		 ABA IAA GA3 CAT PAL Protease SOD SS 综合评价值
Comprehensive evaluation score		 排名
Ranking
RT 0.984 0.750 0.183 0.321 0.244 0.372 0.078 0.505 3.437 3
GT 0.652 0.750 0.496 0.198 0.692 0.390 0.233 0.308 3.719 2
RF 0.652 0.234 0.307 0.124 0.500 0.715 0.233 0.513 3.278 4
GF 1.024 0.624 0.320 0.154 0.322 0.715 0.159 0.771 4.089 1
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