Crops ›› 2016, Vol. 32 ›› Issue (1): 56-61.doi: 10.16035/j.issn.1001-7283.2016.01.011

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The Response and Screening of Germplasm Tolerant to Mixed Saline-Alkali Stress in Sweet Sorghum

Wang Chen,Chen Jibao,Pang Zhenling,Li Nannan,Dong Haihong,Li Dandan   

  1. Institute of Agricultural Engineering,Nanyang Normal University,Nanyang 473061,Henan,China
  • Received:2015-11-19 Revised:2015-12-08 Online:2016-02-15 Published:2018-08-25
  • Contact: Jibao Chen

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Abstract:

Physiological responses of sweet sorghum to mixed saline-alkali stress were evaluated to explore the high saline-alkali tolerance germplasm and provide physiological indicators for identifying saline-alkali tolerance at seedling stage. Two growth characteristics (relative germination rate and fresh weight of per plant) and three physiological characteristics (leaf relative electrical conductivity, proline content and SOD activity) were measured at germination and seedling stages of 5 sweet sorghums under four mixed saline-alkali stress conditions. The saline-alkali tolerance was sorted out using the method of membership function and grey relation method in 5 genotypes. The four stress levels were named as T1, T2, T3 and T4, which Na + concentration were 50mmol/L, 100mmol/L, 200mmol/L, 300mmol/L , respectively, and the pH were 7.12、8.01、8.80 and 10.07, respectively. It was shown that, with the increase of stress, the relative germination and fresh weight decreased, leaf relative electrical conductivity and proline content increased. The SOD activity increased when stress started and reached to the peak at T3, and then decreased. Based on the means of parameters at T3 treatment, the membership function analysis and grey relation analysis were performed. The results showed that the association degree between different indices and comprehensive saline-alkali resistance indices was ranked as follows: proline content (0.95), fresh weight (0.93), leaf relative electrical conductivity (0.92), SOD activity (0.92) and the relative germination (0.74). The saline-alkali tolerance of 5 genotypes were finally ranked as Yajin106> Yajin4589> Yajin2> Yajin4184> Yajin10 based on the membership function analysis and Yajin2> Yajin106> Yajin4589> Yajin4184> Yajin10 based on grey relation analysis. These results indicated that the variation of saline-alkali tolerance was remarkable among the 5 genotypes. The fresh weight, leaf relative electrical conductivity, proline content and SOD activity were the reliable characteristics used for identification of sweet sorghum tolerant to saline-alkali stress. Yajin106 was the most tolerant genotype and Yajin10 was the least tolerant one among the tested materials.

Key words: Sweet sorghum, Mixed saline-alkali stress, Saline-alkali tolerance

Table 1

Component of mixture saline-alkali solution"

处理
Treatment		 1L盐碱混合液各组分母液量
Quantity of each component per liter mixture saline-alkali solution（mL）		 Na+浓度
Na+concentration
（mmol/L）		 盐度*
Salt concentration
(%)		 pH
NaCl Na2SO4 NaHCO3 Na2CO3
T1 25 25 0 0 50 0.30 7.12
T2 25 50 25 0 100 0.60 8.01
T3 10 90 90 10 200 1.20 8.80
T4 75 75 75 75 300 1.80 10.07

Fig.1

Effect of salt stress on the germination rate of sweet sorghum"

Fig.2

Effect of salt stress on the fresh weight of sweet sorghum"

Fig.3

Effect of salt stress on electrical conductivity of sweet sorghum leaves"

Fig.4

Effect of salt stress on proline content of sweet sorghum leaves"

Fig.5

Effect of salt stress on SOD activity of sweet sorghum leaves"

Table 2

Correlation degree and correlation order between different parameters and comprehensive saline-alkali-resistance index of sweet sorghum"

指标Characteristics 关联度Correlation degree 权重Weight 排序Rank
脯氨酸含量Proline content 0.945 0.212 1
茎叶鲜重 Fresh weight of leaf and stem 0.933 0.209 2
相对电导率 Relative electrical conductivity 0.925 0.207 3
SOD活性SOD activity 0.919 0.206 4
相对发芽率Relative germination rate 0.740 0.166 5

Table 3

Saline-alkali-tolerance rank of tested germplasm resources"

材料名称
Material		 加权抗盐碱
指数WDI		 排序
Rank		 D值
D-value		 排序
Rank
雅津2号 Yajin2 2.297 1 0.464 3
雅津106号 Yajin106 1.925 2 0.674 1
雅津4589号 Yajin4589 1.832 3 0.646 2
雅津4184号 Yajin4184 1.505 4 0.426 4
雅津10号 Yajin10 0.960 5 0.034 5
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