Crops ›› 2016, Vol. 32 ›› Issue (1): 149-153.doi: 10.16035/j.issn.1001-7283.2016.01.028

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Study on Total Phenolic Content and Correlation between PPO Activity and Browning of Corydalis saxicola Bunting Callus

Cen Zhongyong,Su Jiang,Deng Xichao,Xie Yanjun   

  1. School of Chemistry and Biological Engineering,Hechi University,Yizhou 546300,Guangxi,China
  • Received:2015-11-04 Revised:2015-12-30 Online:2016-02-15 Published:2018-08-25

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Abstract:

With non-browned callus of Corydalis saxicola as materials, the effects of different concentrations of ascorbic acid on browning of Corydalis saxicola callus, total phenolic content and PPO activity were studied by adding them to culture medium in this experiment. The results showed that the browning rates of Corydalis saxicola bunting callus under different ascorbic acid concentrations were lower than that of the control on the 20th day. While the activities of PPO of all the treatments were higher than that of the control, the activity of PPO with concentration of 175mg/L ascorbic acid was the highest with a value of 14.1400U/(g·min), 72.3% higher than the control. The total phenolic contents of all the treatments were lower than that of the control, and the total phenolic content with concentration of 175mg/L ascorbic acid was the lowest with a value of 0.4633μg/mL, 88.3% lower than the control. The correlation analysis showed that the browning rate of callus was positively correlated with the content of total phenols, and negatively correlated with the activity of PPO. The activity of PPO was much negatively correlated with the total phenol content in the callus.

Key words: Corydalis saxicola, Bunting callus, Browning, Total phenol, PPO

Table 1

The effects of different treatments on the activity of PPO of Corydalis saxicola bunting callus U/(g·min)"

处理
Treatment		 均值
Mean value		 标准差
Standard deviation		 标准误
Standard error		 均值的95%置信区间95% Confidence interval of mean 极小值
Minimum		 极大值
Maximum
下限Lower bound 上限Upper bound
对照（CK） 3.9233 0.11930 0.06888 3.6270 4.2197 3.79 4.02
75mg/L 5.6500 0.26514 0.15308 4.9914 6.3086 5.39 5.92
125mg/L 7.1633 0.19655 0.11348 6.6751 7.6516 6.94 7.31
175mg/L 14.1400 0.25239 0.14572 13.5130 14.7670 13.85 14.31
225mg/L 9.0900 0.20421 0.11790 8.5827 9.5973 8.86 9.25

Table 2

Multi-comparisons of the activity of PPO of Corydalis saxicola bunting callus of different treatments U/(g·min)"

处理
Treatment		 重复Repetition 平均
Average
Ⅰ Ⅱ Ⅲ
对照（CK） 3.79 4.02 3.96 3.9233E
75mg/L 5.92 5.39 5.64 5.6500D
125mg/L 7.24 6.94 7.31 7.1633C
175mg/L 14.31 13.85 14.26 14.1400A
225mg/L 9.25 8.86 9.16 9.0900B

Table 3

Total phenolic content of Corydalis saxicola bunting callus of different treatments μg/mL"

处理
Treatment		 均值
Mean value		 标准差
Standard deviation		 标准误
Standard error		 均值的95%置信区间95% Confidence interval of mean 极小值
Minimum		 极大值
Maximum
下限Lower bound 上限Upper bound
对照（CK） 3.6167 0.33081 0.19099 2.7949 4.4384 3.24 3.86
75mg/L 2.6233 0.15177 0.08762 2.2463 3.0003 2.46 2.76
125mg/L 2.1400 0.08185 0.04726 1.9367 2.3433 2.05 2.21
175mg/L 0.4633 0.07371 0.04256 0.2802 0.6464 0.38 0.52
225mg/L 1.2600 0.09165 0.05292 1.0323 1.4877 1.18 1.36

Table 4

Multi-comparisons of total phenolic content of Corydalis saxicola bunting callus of different treatments μg/mL"

处理
Treatment		 重复Repetition 平均
Average
Ⅰ Ⅱ Ⅲ
对照（CK） 3.24 3.75 3.86 3.62A
75mg/L 2.46 2.65 2.76 2.62B
125mg/L 2.05 2.16 2.21 2.14C
175mg/L 0.38 0.52 0.49 0.46E
225mg/L 1.18 1.36 1.24 1.26D

Table 5

Effects of different treatments on Corydalis saxicola bunting callus browning"

处理
Treatment		 接种愈伤组织数
Inoculated callus number		 褐化愈伤组织数
Number of
brown callus		 褐化率
Browning rate(%)
对照（CK） 111 84 75.68
75mg/L 117 31 27.35
125mg/L 112 26 23.21
175mg/L 115 21 18.26
225mg/L 119 25 21.01

Table 6

Correlation between browning rate,ppo activity and total phenols content of Corydalis saxicola bunting callus"

项目Items 褐变率Browning rate PPO活性PPO activity 总酚含量Total phenols content
褐变率 Browning rate 相关性Pearson 1 -0.654 0.786
显著性（双侧）Sig.(2-tailed) 0.231 0.115
N 5 5 5
PPO活性 PPO activity 相关性Pearson -0.654 1 -0.963**
显著性（双侧）Sig.(2-tailed) 0.231 0.009
N 5 5 5
总酚含量 Total phenols content 相关性Pearson 0.786 -0.963** 1
显著性（双侧）Sig.(2-tailed) 0.115 0.009
N 5 5 5
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