Crops ›› 2016, Vol. 32 ›› Issue (5): 156-159.doi: 10.16035/j.issn.1001-7283.2016.05.027

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Effects of Diplocarpon rosae on the Leaves Ultrastructure of Different Rosa hybrida

Huang Xiaoling,Huang Ze,Zhang Jinzhu,Song Dan,Yang Tao,Zhang Xiaoying,Xiong Yan,Che Daidi   

  1. College of Horticulture,Northeast Agricultural University,Harbin 150030,Heilongjiang,China
  • Received:2016-05-23 Revised:2016-08-16 Online:2016-10-15 Published:2018-08-26
  • Contact: Daidi Che

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Abstract:

Two Rose hybrida offsprings were examined differing in black spot tolerance at the subcellular level. The subcellular structure had no difference between 2 materials under non-stressed conditions. But chloroplasts and mitochondria of leaf cells of both Rose hybrida offsprings showed ultrastructural changes after 6 days of black spot inoculation. Observing with scanning electron microscope, large number of mycelium scattered on the leaf epidermis of resistant offspring, a few hyphae got into the stomata; While the susceptible one’s epidermis swelled and stomata subsidented, spore were floating on the surface. Observing with transmission electron microscope, cells of resistant offspring contained much osmiophilic granules, the shape of chloroplasts changed from oval to round. Thylakoid arranged loosely, directly disordered and its morphology homogeneity was reduced. Chloroplast in cell of susceptible one collapsed, only the vacuole and nucleus were kept, and more vacuoles were appeared. With further infection of pathogenic bacteria, cytoplasmic degraded completely until cells died.

Key words: Rosa hybrida offspring, Black spot, Ultrastructure

Table 1

Hybrid combinations of modern Chinese rose varieties"

杂交组合
Hybrid combination		 母本
Female		 父本
Male		 F1株数
The number of F1
1 紫色美地兰 花仙子 30
2 北京红 12
3 金奖章 15
4 第一玫瑰红 24
5 紫色美地兰 红烈焰 13
6 北京红 14
7 金奖章 20
8 粉和平 17

Table 2

Assessment resistance mm"

病级Grade of disease 病斑直径Disease spot diameter
1 0
2 0<d≤2.0
3 2.0<d≤3.0
4 3.0<d≤4.0
5 4.0<d≤4.5
6 4.5<d≤5.0
7 d>5.0

Table 3

Identification of different resistance Rosa Hybrida’s resistance to black spot pathogen"

序号
No.		 母本
Female		 父本
Male		 平均病斑直径(mm)
Average disease spot diameter		 病斑直径标准差
Standard deviation		 发病率(%)
Incidence		 病情指数(%)
Disease index		 后代抗性
Progeny population grade of disease
1 紫色美地兰(HR) 花仙子(HR) 1.50 0.32 45 11.43 HR
2 北京红(HR) 花仙子(HR) 1.80 0.29 50 15.60 HR
3 金奖章(M) 花仙子(HR) 4.60 0.49 100 53.40 MR
4 第一玫瑰红(S) 花仙子(HR) 3.54 0.38 75 26.60 R
5 紫色美地兰(HR) 红烈焰(M) 3.47 0.28 90 30.00 R
6 北京红(HR) 红烈焰(M) 3.08 0.23 95 32.31 R
7 金奖章(M) 红烈焰(M) 4.80 0.20 100 82.14 S
8 粉和平(LR) 红烈焰(M) 3.06 0.14 70 48.98 MR
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