Crops ›› 2020, Vol. 36 ›› Issue (6): 23-29.doi: 10.16035/j.issn.1001-7283.2020.06.004

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Genome-Wide Association Study of Digestibility of Straw in Maize

Lin Miao1(), Zhang Qiuzhi1, Shi Liyu1, Liu Bei2, Wang Hongwu2, Pan Jinbao1()   

  1. 1Beijing University of Agriculture, Beijing 102206, China
    2Zhongyu Golden Marker (Beijing) Biotechnology Incorporated Company, Beijing 102206, China
  • Received:2020-04-12 Revised:2020-07-08 Online:2020-12-15 Published:2020-12-09
  • Contact: Pan Jinbao E-mail:18404984376@163.com;panjinbao126@126.com

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Abstract:

The 48h dry matter digestibility (48h IVDMD) is an important index to measure the quality of silage maize. In order to explore the molecular genetic mechanism of digestibility of 48h IVDMD, 341 maize inbred lines were evaluated for 48h IVDMD in 2018 in Shenyang and Tongliao. These inbred lines were subjected to whole genome sequencing, generating a total of 6 276 612 high quality SNPs. GWAS analysis detected 153 SNPs using the threshold of P <1.0×10-6 and 4 SNPs using the threshold of P < 1.0×10-8. Thirty-eight candidate genes were identified based on the GWAS, and the biological functions of these genes were mainly involved in cell growth and development, defense reaction, and signal transduction.

Key words: Maize, Straw, 48h dry matter digestibility, Genome-wide association study

Fig.1

Frequency distribution of 48h IVDMD"

Table 1

Statistics analysis of 48h IVDMD in two environments"

地点
Location		 均值
Mean (%)		 变异范围
Range (%)		 变异系数
Coefficient of variation (%)		 偏度
Skewness (%)		 峰度
Kurtosis (%)		 相关系数
Correlation coefficient		 广义遗传力
Broad-sense heritability (%)
沈阳Shenyang
沈阳Shenyang 80.96 67.24~91.41 4.85 -0.64 1.04
通辽Tongliao 67.94 49.75~85.94 9.50 -0.40 -0.24 0.63** 71.98

Fig.2

Manhattan plot of genome-wide association under the environments of Shenyang and Tongliao The solid and dashed lines represent significance thresholds 8.0 and 6.0, respectively"

Fig.3

Quantile-quantile plot of genome-wide association under the environments of Shenyang and Tongliao The red line represents the expected distribution of null hypothesis, and the blue line represents the observed correlation distribution with the percentage of Tongliao straw at 48h IVDMD"

Fig.4

Significant SNPs were distributed on chromosomes"

Fig.5

Manhattan plots and its QQ plots of genome-wide association in two environments (MLM)"

Fig.6

The location of the variant gene"

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