Crops ›› 2020, Vol. 36 ›› Issue (4): 158-163.doi: 10.16035/j.issn.1001-7283.2020.04.022

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Morphological and Physiological Differences of Maize Inbred Lines at Seedling Stage under Waterlogging Stress

Zheng Fei(), Wang Lixia, Liu Ruixiang, Kong Lingjie, Chen Yanping, Yuan Jianhua, Cui Yakun()   

  1. Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Agrobiology of Jiangsu Province, Nanjing 210014, Jiangsu, China
  • Received:2019-10-22 Revised:2019-11-16 Online:2020-08-15 Published:2020-08-11
  • Contact: Cui Yakun E-mail:540061782@qq.com;cuiyakun1920@163.com

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Abstract:

To investigate the effects of waterlogging during seedling stage on morphology and physiology and to evaluate the genotypic differences, thirty-five maize inbred lines were used. The results showed that morphological and physiological traits were significantly affected by waterlogging stress. Principle component analysis indicated that SPAD, leaf area, plant height, root biomass, stem biomass, leaf biomass and total biomass had more proportion. The waterlogging tolerance indexes of total biomass, leaf biomass, stem biomass, root biomass and leaf area could be used for screening superior genotypes. The thirty-five maize inbred lines were classified into three groups as sensitive (seventeen inbred lines), mean type (six inbred lines) and tolerant (twelve inbred lines).

Key words: Maize, Seedling stage, Morphology, Physiology, Waterlogging stress

Table 1

The tested maize inbred lines in the experiment"

编号Code 材料名称Material name 备注Note 编号Code 材料名称Material name 备注Note
1 HCL645 迪卡517母本 19 JS131010 JS06730改良系
2 PH6WC 先玉335母本 20 JS111067 齐319改良系
3 郑58 Zheng 58 郑单958母本 21 JS161111 JS06730改良系
4 JS161022 Su95-1改良系 22 JS141073 JS06730改良系
5 苏95-1 Su 95-1 苏玉29母本 23 JS131061 JS06730改良系
6 JS131160 Su95-1改良系 24 JS161199 JS06730改良系
7 JS161131 Su95-1改良系 25 JS161116 JS06730改良系
8 JS161527 Su95-1改良系 26 JS141043 JS06730改良系
9 JS161045 Su95-1改良系 27 JS141045 JS06730改良系
10 JS161133 Su95-1改良系 28 JS161474 JS06730改良系
11 JS161136 Su95-1改良系 29 JS141027 JS06730改良系
12 JS161137 Su95-1改良系 30 JS06730 苏玉39父本
13 JS161061 Su95-1改良系 31 PH4CV 先玉335父本
14 JSD1189 Su95-1改良系 32 D1798Z 迪卡517父本
15 JS131089 JS06730改良系、苏科玉1417父本 33 JS151181 JS06730改良系
16 JS121042 JS06730改良系、苏科玉1409父本 34 昌7-2 Chang 7-2 郑单958父本
17 JS141004 JS06730改良系 35 齐319 Qi 319 鲁单981母本
18 JS161107 JS06730改良系

Table 2

Effects of waterlogging stress on morphological and physiological indexes in maize inbred lines at seedling stage"

指标
Index		 对照Control 淹水Waterlogging 耐涝指数Waterlogging tolerance index
最大值
Maximum		 最小值
Minimum		 平均值
Mean		 变异系数
Variable coefficient (%)		 最大值
Maximum		 最小值Minimum 平均值
Mean		 变异系数
Variable coefficient (%)		 最大值
Maximum		 最小值Minimum 平均值Mean 变异系数
Variable coefficient (%)
φPSⅡ 0.64 0.50 0.56A 6.75 0.61 0.41 0.52B 10.61 1.01 0.75 0.92 7.77
NPQ 0.28 0.06 0.19B 30.09 0.44 0.10 0.26A 31.21 2.30 1.01 1.42 26.75
SPAD 60.51 40.89 53.47A 9.12 47.99 27.77 39.70B 11.77 1.03 0.51 0.75 13.37
叶片厚度
Leaf thickness (mm)		 1.81 1.42 1.61A 6.94 1.72 1.10 1.45B 10.88 1.15 0.67 0.90 12.44
叶面积(cm2/株)
Leaf area (cm2/plant)		 338.70 128.70 228.70A 21.99 170.70 55.50 108.30B 25.37 0.71 0.32 0.48 18.01
株高Plant height (cm) 55.30 34.50 43.60A 10.42 40.00 21.60 30.90B 15.10 0.84 0.55 0.71 10.17
根长Root length(cm) 53.70 25.60 37.00A 19.96 31.10 12.90 22.50B 20.21 1.00 0.39 0.62 18.64
根数Root number 22.00 9.30 13.40A 19.24 20.70 8.30 13.90A 20.02 1.57 0.69 1.04 17.80
根干物重(g/株)
Root biomass (g/plant)		 0.73 0.18 0.43A 32.33 0.30 0.07 0.15B 35.70 0.59 0.20 0.36 28.28
茎干物重(g/株)
Stem biomass (g/plant)		 0.46 0.15 0.31A 28.06 0.41 0.11 0.24B 33.72 0.98 0.54 0.78 18.06
叶干物重(g/株)
Leaf biomass (g/plant)		 0.73 0.25 0.49A 24.56 0.39 0.11 0.24B 31.52 0.80 0.33 0.49 25.77
总干物重(g/株)
Total biomass (g/plant)		 1.80 0.60 1.20A 25.39 1.09 0.30 0.63B 32.10 0.75 0.33 0.51 21.60
根冠比Root/shoot ratio 0.78 0.25 0.54A 21.71 0.44 0.24 0.31B 16.33 1.13 0.36 0.60 23.36

Table 3

Principle component analysis on 13 waterlogging tolerance indexes"

耐涝指数
Waterlogging tolerance index		 因子1
Factor 1		 因子2
Factor 2		 因子3
Factor 3		 因子4
Factor 4
φPSⅡ 0.21 -0.77 0.46 -0.02
NPQ -0.31 0.68 -0.54 0.08
SPAD 0.66 -0.31 0.14 0.38
叶片厚度Leaf thickness 0.22 -0.11 -0.15 0.89
叶面积Leaf area 0.77 0.09 -0.03 -0.29
株高Plant height 0.67 -0.21 0.02 -0.33
根长Root length 0.15 0.35 0.68 0.00
根数Root number 0.52 -0.05 -0.43 -0.22
根干物重Root biomass 0.74 0.58 0.23 0.06
茎干物重Stem biomass 0.83 -0.01 -0.22 -0.03
叶干物重Leaf biomass 0.93 -0.03 -0.15 0.07
总干物重Total biomass 0.95 0.21 -0.07 0.06
根冠比Root/shoot ratio 0.06 0.78 0.48 0.06
特征值Eigenvalue 4.96 2.34 1.56 1.20
贡献率Contribution rate (%) 38.15 17.97 11.97 9.20
累计贡献率
Cumulative contribution rate (%)		 38.15 56.11 68.09 77.29

Table 4

Correlation analysis of morphological and physiological indexes under waterlogging stress at maize seedling stage"

指标
Index		 φPSⅡ NPQ SPAD 叶片厚度
Leaf thickness		 叶面积
Leaf
area		 株高
Plant
height		 根长
Root
length		 总根数
Root
number		 根干物重
Root
biomass		 茎干物重
Stem
biomass		 叶干物重
Leaf
biomass		 总干物重
Total
biomass
NPQ -0.80**
SPAD -0.35* -0.41*
叶片厚度Leaf thickness -0.04 -0.03 -0.37*
叶面积Leaf area -0.12 -0.23 -0.22 0.03
株高Plant height -0.21 -0.30 -0.53** -0.11 0.44**
根长Root length -0.01 -0.13 -0.00 -0.01 0.14 -0.06
根数Root number -0.07 -0.10 -0.22 0.10 0.50** -0.35* -0.11
根干物重Root biomass -0.15 -0.02 -0.38* 0.08 0.56** -0.34* -0.33 -0.21
茎干物重Stem biomass -0.13 -0.06 -0.49** 0.10 0.50** -0.55** -0.02 -0.34* 0.58**
叶干物重Leaf biomass -0.16 -0.22 -0.55** 0.29 0.75** -0.52** -0.09 -0.50** 0.61** -0.78**
总干物重Total biomass -0.04 -0.10 -0.53** 0.22 0.71** -0.51** -0.15 -0.41* 0.82** -0.87** -0.92**
根冠比Root/shoot tatio -0.34* -0.21 -0.04 -0.10 0.07 -0.12 -0.42* -0.13 0.68** -0.13 -0.09 0.16

Fig.1

System clustering of 35 maize inbred lines"

Table 5

Differences of waterlogging tolerance at seedling stage among three maize inbred lines"

类型
Type		 耐涝指数Waterlogging tolerance index
叶干物重
Leaf
biomass		 总干物重
Total
biomass		 茎干物重
Stem
biomass		 叶面积
Leaf
area		 根干物重
Root biomass
敏感型
Sensitive type		 0.39B 0.42C 0.65B 0.42B 0.29B
中间型
Mean type		 0.48B 0.53B 0.87A 0.48B 0.37AB
耐涝型
Tolerant type		 0.63A 0.63A 0.90A 0.56A 0.44A
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