Crops ›› 2021, Vol. 37 ›› Issue (6): 193-198.doi: 10.16035/j.issn.1001-7283.2021.06.031

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Analysis of Bacterial Community and Influencing Factors in Tobacco Soil at Different Altitudes in Zunyi

Li Maosen1(), Gao Weikai1,2, Ren Tianbao1, Jiang Shixiang3, He Xiaoya3, Luo Leqin3, Yun Fei1(), Ke Xiaoting2()   

  1. 1 Tobacco college of Henan Agricultural University/Henan Engineering Research Center for Biochar/Henan Engineering Laboratory of Biochar Technology, Zhengzhou 450002, Henan, China
    2 China Tobacco Guangdong Industrial Co., Ltd., Guangzhou 510032, Guangdong, China
    3Guizhou Provincial Tobacco Company Zunyi Branch, Zunyi 563000, Guizhou, China
  • Received:2021-05-18 Revised:2021-08-14 Online:2021-12-15 Published:2021-12-16
  • Contact: Yun Fei,Ke Xiaoting E-mail:18134433972@163.com;yunfeifei55@126.com;kytown85@163.com

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Abstract:

In order to explore the characteristics and the main influencing factors of bacterial community structure of tobacco planting soil at different altitudes, three tobacco planting soils at different altitudes of 800 (ZL), 1000 (ZS) and 1200m (ZH) were selected in Zunyi, Guizhou, and the bacterial community of tobacco planting soil was analyzed by 16S rRNA high-throughput sequencing. The results showed that bacteria in tobacco planting soil in ZL and ZH treatments α-diversity were lower than that of ZS treatment. The dominant bacteria phyla in tobacco growing soil at three altitudes were Actinobacteria, Proteobacteria, Chloroflexi and Acidobacteria. The relative abundance of Actinobacteria was the highest in ZL (34.22%), which was higher than that in ZH; on the contrary, the abundance of Proteobacteria in ZH was significantly higher than that in ZL. The abundance of Chloroflexi and Acidobacteria were consistent at the three altitudes, which were ZS > ZL > ZH. The results of cluster heat map analysis showed that soil water content and pH value had a significant positive correlation with Armimonadota and Cyanobacteria, and a significant negative correlation with Proteobacteria; Proteobacteria, Firmicutes and Patescibacteria were significantly correlated with microbial biomass N, but negatively correlated with the content of available K.

Key words: Tobacco-planting soil, Altitude, Bacterial community, Environmental factors

Table 1

Physicochemical indexes of tobacco planting soil at different sample sites"

取样地
Sampling site		 海拔
Altitude (m)		 地理坐标
Geographical coordinates		 pH 容重
Soil bulk density
(g/cm3)		 温度
Temperature
(℃)		 含水率
Water content
(%)
江北咀Jiangbeizui 819.0 106°42′51′′ E,27°27′17′′ N 5.01±0.081c 1.02±0.010b 29.73±0.12c 12.55±1.21c
新华村Xinhua 1022.3 106°36′47′′ E,27°36′49′′ N 7.40±0.087a 1.13±0.049a 31.20±0.62b 19.73±1.70a
新土村Xintu 1220.0 106°38′13′′ E,E27°41′3′′ N 6.23±0.017b 1.18±0.038a 32.67±0.80a 15.51±0.59b
取样地
Sampling site		 有机碳
Soil organic
carbon (g/kg)		 碱解氮
Alkaline N (mg/kg)		 有效磷
Available P
(mg/kg)		 速效钾
Available K
(mg/kg)		 微生物量碳
Microbial biomass
carbon (mg/kg)		 微生物量氮
Microbial biomass
N (mg/kg)
江北咀Jiangbeizui 23.1±0.1b 155.02±6.42a 56.99±9.69a 115.45±0.00c 351.70±33.42b 32.91±4.79a
新华村Xinhua 24.0±0.1a 67.52±7.15b 38.40±4.22b 228.26±2.33b 474.24±10.35a 22.75±2.43b
新土村Xintu 20.1±0.1c 154.37±1.31a 64.81±6.35a 381.79±18.66a 186.60±11.90c 15.52±0.58c

Fig.1

OTUs number of soil bacterial community at different altitudes"

Table 2

Effects of altitude on α diversity of soil bacterial community"

处理Treatment Simpson指数Simpson index Shannon指数Shannon index Chao指数Chao index 覆盖度Coverage
ZH 0.0039±0.00091a 6.38±0.34b 2699.25±634.72b 0.98±0.00370a
ZS 0.0029±0.00053b 6.71±0.11a 3276.21±131.29a 0.98±0.00062a
ZL 0.0036±0.00042ab 6.56±0.11ab 3117.52±200.52ab 0.98±0.00230a

Fig.2

Relative abundance of bacterial community on phylum level"

Fig.3

Comparison of relative abundance of bacterial communities on phylum level between ZL and ZH treatment"

Fig.4

Principal component analysis of soil bacterial community structure"

Fig.5

Correlation between bacterial dominant phylum and environmental factors MBN: microbial biomass N; SOC: soil organic carbon; MBC: microbial biomass carbon; AN: alkaline N; AP: available P; SWC: soil water content; SBD: soil bulk density; T: temperature; AK: available K. “*”indicates P < 0.05, “**”indicates P < 0.01, “***”indicates P < 0.001"

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