Crops ›› 2022, Vol. 38 ›› Issue (3): 181-186.doi: 10.16035/j.issn.1001-7283.2022.03.026

Previous Articles     Next Articles

Endogenous Hormones and Enzyme Activity Analysis in Sugarcane Varieties with Different Perennial Root Ages

Cheng Qin(), Tan Qinliang, Li Jiahui, Zhu Pengjin, Zhou Quanguang, Ou Kewei, Lu Yefei, Lü Ping, Pang Xinhua()   

  1. Guangxi Subtropical Crops Research Institute, Nanning 530001, Guangxi, China
  • Received:2021-04-02 Revised:2021-08-30 Online:2022-06-15 Published:2022-06-20
  • Contact: Pang Xinhua E-mail:389208914@qq.com;gxpxh@126.com

RichHTML

3

PDF (PC)

165

Abstract:

There is a big difference in ratooning ability in different sugarcane varieties. To study the performance of sugarcane varieties in different ratoon years, soluble sugar, soluble protein, endogenous hormone contents and related enzyme activities of roots, stems and leaves at perennial root germination stage were measured using Guire (GR2) and ROC22. The results showed that, the main concentration of soluble sugar and soluble protein were in the stems and leaves and the highest content of soluble sugar was found in the stems of the five-year perennial sugarcane GR2; the largest decline of ABA content was in GR2 which was the most related to ratooning ability, GA3 was negatively correlated with the ratooning ability which was contrary to the trend of ABA and IAA; the plant catalase (CAT) and phenylalanine ammonialyase (PAL) were positively correlated with the ratooning ability, while the sucrose synthase (SS) was negatively correlated with the ratooning ability. The activity of protease was the highest in GR2 of five-year ratooning; the weight coefficients of ABA, IAA, GA3, CAT, PAL, protease, SOD and SS were 0.186, 0.136, 0.107, 0.074, 0.115, 0.165, 0.056, 0.159 by the pole ordering method, respectively. Among them, the weight coefficients of ABA content, protease and SS activities were higher, indicating that the three indexes had a greater impact on the ratooning ability; the ratooning ability were ranked as GF > GT > RT > RF, so GR2 was worth of popularizing because of stronger ratooning ability.

Key words: Sugarcane, Ratooning ability, Endogenous hormone, Enzyme activity, Pole ordering method

Table 1

Agronomic and yield traits of sugarcane ratoon for two and five years"

编号
Number		 株高
Plant height
(cm)		 茎径
Stem diameter
(cm)		 单茎重
Productive
stalk (kg)		 小区有效茎数
Effective stems
per plot		 有效茎数
Effective stem
(×104/hm2)		 小区产量
Grain yield
per plot (kg)		 产量
Yield
(kg/hm2)		 田间锤度
Hammer
degree (%)		 蔗糖分
Sugar
(%)		 含糖量
Sugar content
(kg/hm2)
GT 345aA 2.64aA 1.72aA 312cC 8.68aA 435.6cC 121 020aA 19.83aA 13.77aA 16 605aA
RT 330aA 2.59aA 1.59aA 269cC 7.48bAB 299.8dD 83 280bB 20.63aA 14.63aA 12 210bB
GF 341aA 2.80aA 1.92aA 564aA 8.48aA 814.1aA 122 325aA 19.87aA 13.80aA 16 875aA
RF 321aA 2.80aA 1.80aA 430bB 6.47cB 588.2bB 88 380bB 20.10aA 14.06aA 12 375bB

Fig.1

Soluble sugar and protein contents"

Fig.2

Endogenous hormone contents of root, stem and leaf at sprouting stage Different capital and lowercase letters indicate significant difference at 0.01 and 0.05 level, respectively, the same below"

Table 2

Variance analysis of endogenous hormones for roots"

编号Number ABA (μg/L) IAA (ng/L) GA3 (pg/mL)
RT 212.73±1.89cC 277.89±3.62dD 448.01±2.67aA
GT 352.86±2.50aA 430.54±3.62aA 338.88±4.01cC
RF 287.86±3.00bB 320.42±2.49cC 356.38±2.84bB
GF 210.14±2.75cC 342.44±2.37bB 330.33±1.77cC

Fig.3

The enzyme activities of root, stem and leaf at sprouting stage"

Table 3

The variance analysis of enzyme activity for roots"

编号Number CAT (U/mL) PAL (U/L) Protease (U/L) SOD (U/L) SS (U/mL)
RT 7.40±0.03dD 46.13±0.57aA 471.75±5.57bB 3730.93±377.40bA 11.68±0.15dD
GT 7.77±0.12cC 41.48±0.24bB 347.69±7.61cC 3573.41±41.50bA 18.17±0.22aA
RF 8.03±0.08bB 32.07±0.18cC 328.50±4.08dD 4128.14±41.50aA 12.53±0.04cC
GF 9.31±0.07aA 45.93±0.66aA 524.42±2.04aA 3888.44±57.10abA 17.25±0.29bB

Table 4

Scores of root of two varieties in different measurement indexes during perennial root germination stage (evaluation matrix)"

编号Number ABA IAA GA3 CAT PAL Protease SOD SS
RT 5.28 5.50 1.71 4.33 2.11 2.25 1.38 3.17
GT 3.50 5.50 4.64 2.67 6.00 2.36 4.12 1.93
RF 3.50 1.71 2.87 1.67 4.33 4.33 4.12 3.22
GF 5.50 4.58 3.00 2.07 2.79 4.33 2.82 4.84

Table 5

Comprehensive evaluation of perennial roots at germination stage"

编号
Number		 ABA IAA GA3 CAT PAL Protease SOD SS 综合评价值
Comprehensive evaluation score		 排名
Ranking
RT 0.984 0.750 0.183 0.321 0.244 0.372 0.078 0.505 3.437 3
GT 0.652 0.750 0.496 0.198 0.692 0.390 0.233 0.308 3.719 2
RF 0.652 0.234 0.307 0.124 0.500 0.715 0.233 0.513 3.278 4
GF 1.024 0.624 0.320 0.154 0.322 0.715 0.159 0.771 4.089 1
[1] 侯佳. 广西蔗糖产业发展现状与分析. 新经济, 2014(2):18-20.
[2] 马丽, 应雄美, 刘新龙, 等. 17份国内甘蔗种质三年宿根性的产质量评价. 中国糖料, 2010(3):33-34.
[3] 韦开军, 邓思, 阳康春, 等. 6个甘蔗优良新品种(系)宿根性状结果分析. 中国种业, 2018(11):68-70.
[4] 丁雪儿, 林萍萍, 余凡, 等. 甘蔗宿根性研究进展. 中国糖料, 2020, 42(2):12-18.
[5] 邓祖湖, 林彦铨. 不同选择方法对甘蔗宿根性的选择效果分析. 福建农业科技, 2000(S1):60-61.
[6] 马丽, 蔡青, 应雄美, 等. 五年宿根蔗种质材料的产量、糖分评价. 中国糖料, 2008(2):33-34.
[7] 覃伟, 吴才文, 姚丽, 等. 甘蔗宿根萌发期內源激素变化与其宿根性的关系. 西北植物学报, 2014, 34(1):143-149.
[8] 杨荣仲. 甘蔗宿根性与抗寒性初探. 甘蔗糖业, 1996(6):13-17.
[9] Milligan S, Martin F, Gravois K. Inheritance of sugarcane ratooning ability and the relationship of younger crop traits to older crop traits. Crop Science, 1996, 36(1):45-50.
doi: 10.2135/cropsci1996.0011183X003600010008x
[10] 覃伟, 吴才文, 赵俊, 等. 甘蔗宿根性研究I. 甘蔗宿根蔗蔸形态特征与其宿根性的关系. 西南农业学报, 2017, 30(5):989-993.
[11] 覃伟, 吴才文, 赵俊, 等. 甘蔗宿根性研究II. 甘蔗种性宿根影响因子与其宿根性的关系. 中国糖料, 2017, 39(1):1-3.
[12] 唐仕云, 杨丽涛, 李杨瑞. 不同甘蔗基因型对低温胁迫的响应及其耐寒性评价. 热带作物学报, 2012, 33(4):635-641.
[13] 潘晓雪, 胡明瑜, 王忠伟, 等. 不同水稻种质资源重要农艺性状与发芽期耐寒性鉴定研究. 作物杂志, 2021(1):47-53.
[14] 戴海芳, 武辉, 阿曼古丽·买买提阿力, 等. 不同基因型棉花苗期耐盐性分析及其鉴定指标筛选. 中国农业科学, 2014, 47(7):1290-1300.
[15] 王丽艳, 唐金敏, 郑桂萍, 等. 水稻萌发期和幼苗期耐低温指标体系构建及综合评价. 中国农业科技导报, 2019, 21(10):58-65.
[16] 邓祖湖, 林彦铨. 不同选择方法对甘蔗宿根性的选择效果分析. 福建农业科技, 2000(S1):60-61.
[17] 李杨瑞. 现代甘蔗学. 北京: 中国农业出版社, 2010.
[18] 覃伟, 吴才文, 曾千春, 等. 用新台糖甘蔗品种作母本培育强宿根后代的潜力评价. 湖南农业大学学报(自然科学版), 2012, 38(1):1-7.
[19] 马丽, 应雄美, 刘新龙, 等. 34个国外引进甘蔗种质三年宿根性产质量评价. 中国糖料, 2010(2):31-32,36.
[20] 方位宽. 影响宿根甘蔗萌发的若干因素探讨. 南宁:广西大学, 2003.
[21] 苏俊波, 孔冉, 罗炼芳, 等. 甘蔗机械化收获后的宿根性能分析. 甘蔗糖业, 2016(6):22-28.
[22] 宋奇琦, Singh P, Singh R K, 等. 甘蔗应答黑穗病菌侵染相关酶活性和内源激素含量的变化. 南方农业学报, 2018, 49(4):650-655.
[23] 王川, 谢惠民, 王娜, 等. 小麦品种可溶性糖和保护性酶与抗旱性关系研究. 干旱地区农业研究, 2011, 29(5):94-99.
[24] 杨贵先. 南瓜花发育过程中内源激素、多胺和酶活的变化研究. 哈尔滨:东北农业大学, 2015.
[25] 周慧文, 陈荣发, 范业赓, 等. 不同施氮水平下甘蔗内源激素、产量和糖分的变化特征. 热带作物学报, 2019, 40(11):2142-2148.
[26] 何静丹. 低温胁迫及温度回升对甘蔗生理生化指标的影响. 南宁:广西大学, 2011.
[27] 李鸿博, 蔡伟俊, 谢雨彤, 等. 甘蔗新品系对干旱胁迫的生理响应及抗旱性评价. 华南农业大学学报, 2019, 40(6):51-58.
[1] Zhou Wuxian, Li Mengge, Tan Xuhui, Wang Youyuan, Wang Hua, Jiang Xiaogang, Duan Yuanyuan, Zhang Meide. Effects of Sowing Density on Growth, Nutritional Quality and Soil Enzyme Activity of Pinellia ternata in Different Seasons [J]. Crops, 2022, 38(4): 205-213.
[2] Yan Peng, Dong Xuerui, Lu Lin, Fang Mengying, Li Yijie, Wang Weizan, Dong Zhiqiang. Effects of NAA/KT Presoaking on Sugarcane Yield, Root Development and Lodging Resistance [J]. Crops, 2022, 38(4): 99-106.
[3] Wei Xiaokai, Jing Yanqiu, He Jixian, Gu Huizhan, Lei Qiang, Yu Shikang, Zhang Qili, Li Junju. Alleviating Effect of Exogenous Spermidine on Flue-Cured Tobacco Seedlings under Drought Stress [J]. Crops, 2022, 38(3): 143-148.
[4] Tan Qinliang, Cheng Qin, Pan Chenglie, Zhu Pengjin, Li Jiahui, Song Qiqi, Nong Zemei, Zhou Quanguang, Pang Xinhua, Lü Ping. Effects of Drought Stress on Physiological Indexes of New Sugarcane Variety Guire 2 [J]. Crops, 2022, 38(3): 161-167.
[5] Guo Shuya, Shang Shang, Tang Qining, Zhang Yan, Lu Guangyuan. Effects of Different Rotation Tillage Methods and Biochar on Soil Enzyme Activity, Soil Nutrients and Yield of Wheat and Maize [J]. Crops, 2022, 38(3): 211-217.
[6] Liu Limin, Liu Hongjian, Li Aomei, He Weizhong. Effects of Light and Temperature on Photoautotrophic Rooting for in Vitro Sugarcane Plantlets [J]. Crops, 2022, 38(2): 153-157.
[7] Luo Hanmin, Xiong Faqian, Qiu Lihang, Liu Jing, Duan Weixing, Gao Yijing, Qin Xiayan, Wu Jianming, Li Yangrui, Liu Junxian. Application Study of Molecular Markers Associated with Traits in Sugarcane Molecular Breeding [J]. Crops, 2022, 38(2): 35-43.
[8] Zhang Siwei, Li Jin’ao, Liu Boyuan, Jiang Yuchen, Zhong Qiu, Lei Yunkang, Zhang Mingyue, Zhao Mingqin. Effects of Topping Method on Nitrogen Accumulation and Quality of Cigar Tobacco Leaves [J]. Crops, 2022, 38(1): 184-189.
[9] Li Jiahui, Cheng Qin, Ou Kewei, Tan Qinliang, Pang Xinhua, Zhou Quanguang, Lü Ping, Song Qiqi, Tang Yuwei, Zhu Pengjin. Comparison of Tiller Characters of Sugarcane Varieties (Lines) in Different Sugarcane Regions and Their Effects on Yield and Yield Components [J]. Crops, 2021, 37(5): 79-86.
[10] Guo Qiang, Ma Wenqing, Qin Changxian, Shi Zesheng, Peng Chong, Bi Dejin, He Hongliang, Liang Yongjian, Tang Liqiu. Comprehensive Evaluation of New Sugarcane Clones by DTOPSIS Method [J]. Crops, 2021, 37(4): 32-37.
[11] Wang Huifang, Zhang Xi, Feng Xiaohu, Li Yifan, Zhang Hong, Zhao Songchao, Zhao Mingqin. Effects of Different Plant Growth Regulators on the Growth and Development of Flue-Cured Tobacco [J]. Crops, 2021, 37(3): 173-177.
[12] Wu Qihua, Chen Diwen, Zhou Wenling, Ao Junhua, Huang Ying, Huang Zhenrui, Li Shuang, Sun Donglei. Effects of Reducing Phosphorus Application in High-P Soils on the P Efficiency of Chewing Cane and Soil Enzyme Activity [J]. Crops, 2021, 37(3): 91-98.
[13] Fan Yegeng, Chen Rongfa, Yan Haifeng, Zhou Huiwen, Weng Mengling, Huang Xing, Luo Ting, Zhou Zhongfeng, Qiu Lihang, Wu Jianming. Effects of Sugarcane Rotation Green Fodder Corn and Peanut on Sugarcane Growth and Soil Properties [J]. Crops, 2021, 37(1): 104-111.
[14] Xu Lin, Wu Kaichao, Pang Tian, Deng Zhinian, Zhang Ronghua, Huang Chengfeng, Huang Hairong, Li Yijie, Liu Xiaoyan, Qin Wenxian, Wang Weizan. Effects of Root Promoting Agent on the Development and Yield in Single Bud of Sugarcane [J]. Crops, 2020, 36(6): 132-136.
[15] Xie Jinlan, Lin Li, Li Changning, Luo Ting, Mo Zhanghong. Effects of Intercropping Mungbean Straw Returning on Sugarcane Growth and Nitrogen Metabolism under Nitrogen Fertilizer Reduction [J]. Crops, 2020, 36(4): 164-169.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!