Crops ›› 2022, Vol. 38 ›› Issue (3): 205-210.doi: 10.16035/j.issn.1001-7283.2022.03.030

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Effects of Different Root-Promoting Practices on Potassium Metabolism at Mature Stage of Flue-Cured Tobacco in Southern Anhui

Zhang Xiaoquan1(), Jia Zhenyu1, Li Juxu1, Li Hongchen2, Wang Baoxiang3, Wang Jian3, Shi Gang3, Wang Chuan4, Wu Yunjie1()   

  1. 1College of Tobacco Sciences, Henan Agricultural University, Zhengzhou 450002, Henan, China
    2Sanmenxia Company of Henan Tobacco Corporation, Sanmenxia 472000, Henan, China
    3China Tobacco Hubei Industrial Co., Ltd., Wuhan 430040, Hubei, China
    4Anhui Wannan Tobacco Co., Ltd., Xuancheng 242000, Anhui, China
  • Received:2021-09-02 Revised:2021-09-13 Online:2022-06-15 Published:2022-06-20
  • Contact: Wu Yunjie E-mail:zxq013415@163.com;wuyunjie6@163.com

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Abstract:

The effects of different root-promoting practices on root physiology, potassium content in dried leaves at maturity stage, potassium accumulation in different plant parts, expression of potassium channel genes (NKT1, NtKC1, NtORK1, NtTPK1) and transporter genes (NtHAK1, NtKT12) were studied between treatments and application of fulvic acid-potassium (TPFA), γ-polyglutamic acid (TPGA) and soybean milk irrigation (TSRI), and using normal insemination (CK) as control. Results showed that root fresh weight, root dry weight and root volume of all treatments were significantly higher than that of CK, root activities were significantly higher in TPFA and TSRI treatments than that of TPGA treatment. TSRI and TPGA treatments could significantly increase the potassium content of flue-cured tobacco leaves. Various root-promoting practices could significantly promote potassium accumulation, particularly in the root, stem, and middle leaf of flue-cured tobacco. The relative expression of NtORK1 in the roots of the TSRI treatment was significantly lower than CK treatment. The relative expression of NtORK1 in the upper and middle leaves of TPGA and TPFA treatments were significantly lower than that of CK treatment. The relative expression of NtTPK1 in roots of TSRI treatment was significantly higher than other treatments. The relative expression of NtHAK1 in roots of three treatments were significantly increased compared with CK treatment. In conclusion, TPGA and TSRI treatments could promote root development and potassium accumulation of tobacco plants, and improve absorption and transport capacity of tobacco plants at mature stage, and finally improve potassium content of flue-cured tobacco leaves.

Key words: Flue-cured tobacco, Root-promoting practices, Mature stage, Potassium accumulation, Gene expression

Table 1

Root physiological characteristics of flue-cured tobacco with different treatments at mature stage"

处理
Treatment		 根鲜重
Root fresh weight (g)		 根干重
Root dry weight (g)		 根体积
Root volume (cm3)		 根系活力
Root activity [μg/(g FW·h)]
CK 206.41±2.36Cd 61.48±0.86Bc 186.00±7.07Cc 225.17±4.56BCb
TPFA 244.85±2.37BCc 110.48±5.53Aab 361.50±2.12Aa 320.97±8.67Aa
TPGA 368.15±20.92Aa 103.97±3.15Ab 384.00±12.73Aa 169.68±3.65Cc
TSRI 282.19±18.55Bb 121.23±2.87Aa 264.00±19.80Bb 285.16±27.37ABa

Table 2

Potassium contents in different parts of flue-cured tobacco with different treatments at mature stage %"

处理Treatment 上部叶Upper leaves 中部叶Middle leaves 下部叶Lower leaves 茎Stem 根Root
CK 1.47±0.09Aa 1.45±0.09Aa 1.39±0.02Ab 1.91±0.19Bc 1.17±0.04Ab
TPFA 1.41±0.09Aa 1.47±0.05Aa 1.49±0.07Aa 1.97±0.04ABbc 1.16±0.07Ab
TPGA 1.43±0.07Aa 1.51±0.13Aa 1.43±0.09Aab 2.11±0.10Aa 1.23±0.02Aa
TSRI 1.48±0.09Aa 1.53±0.03Aa 1.38±0.04Ab 2.06±0.08ABab 1.15±0.05Ab

Table 3

"

处理Treatment 上部叶Upper leaves 中部叶Middle leaves 下部叶Lower leaves 茎Stem 根Root 单株总量Total of plant
CK 20.12±2.58Aa 53.02±1.70Cc 22.21±0.51Bb 73.07±2.59Cd 61.48±0.86Bc 229.90±3.83Dd
TPFA 23.74±0.53Aa 67.00±2.38Bb 14.12±1.65Cd 104.23±1.55Bc 110.48±5.53Aab 319.56±0.59Cc
TPGA 25.94±5.28Aa 85.58±2.85Aa 34.54±0.03Aa 120.62±0.07Aa 103.97±3.15Ab 370.64±0.62Aa
TSRI 24.80±0.11Aa 65.84±3.15Bb 17.72±0.30Cc 110.51±0.99Bb 121.23±2.87Aa 340.09±0.31Bb

Table 4

"

处理Treatment 上部叶Upper leaves 中部叶Middle leaves 下部叶Lower leaves 茎Stem 根Root 单株总量Total of plant
CK 0.29±0.04Aa 0.77±0.02Cc 0.31±0.01Bb 1.39±0.05Dd 0.72±0.01Bb 3.48±0.07Dd
TPFA 0.33±0.01Aa 0.99±0.04Bb 0.21±0.02Cd 2.05±0.03Cc 1.28±0.06Aa 4.86±0.03Cc
TPGA 0.37±0.08Aa 1.29±0.04Aa 0.49±0.00Aa 2.54±0.00Aa 1.28±0.04Aa 5.98±0.00Aa
TSRI 0.37±0.00Aa 1.01±0.05Bb 0.24±0.00BCc 2.27±0.02Bb 1.40±0.03Aa 5.28±0.00Bb

Table 5

Potassium content of flue-cured tobacco leaves with different treatments %"

处理
Treatment		 上部叶
Upper leaves		 中部叶
Middle leaves		 下部叶
Lower leaves
CK 1.16±0.06Bb 1.07±0.03Cc 1.47±0.04Cc
TPFA 1.17±0.08Bb 1.18±0.01Bb 1.47±0.07Cc
TPGA 1.18±0.03Bb 1.17±0.02Bb 1.52±0.05Bb
TSRI 1.31±0.04Aa 1.32±0.04Aa 1.60±0.11Aa

Fig.1

The relative expression levels of potassium ion channel genes in different parts of tobacco at mature stage of different treatments The upper and lowercase letters indicate significant difference at the P < 0.01 and P < 0.05 level, the same below"

Fig.2

The relative expression levels of potassium transporter genes in different parts of tobacco at mature stage of different treatments"

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