Table 1

Primers sequence and application"

Table 2

Bioinformatics analysis tools"

Fig.1

Schematic diagram of different tissues of lavender flower organs and different development stages"

Fig.2

Agarose gel electrophoresis of LIS gene amplification products"

Fig.3

Conserved domains in LIS1 and LIS2 proteins"

Fig.4

Predicted secondary structure of LIS1 and LIS2 proteins"

Fig.5

Predicted tertiary structures of LIS1 and LIS2 proteins"

Fig.6

Multiple alignment of amino acid sequences of LIS1 and LIS2 proteins with linalool synthase proteins from other species The first red box line is RRX8W functional group, the second red box line is DDXXD functional group, and the third red box line is DDXXTXXXE functional group"

Fig.7

Phylogenetic tree analysis of LIS1 and LIS2 proteins The numerical values of the branch points represent the confidence of the bootstrap validation based on 1000 repetitions"

Fig.8

LIS1 gene expression level and significance analysis Different letters indicate significant difference at 0.05 level, the same below"

Fig.9

LIS2 gene expression level and significance analysis"

Fig.10

Identification of recombinant plasmid pET-28a(+)-LIS1 and pET-28a(+)-LIS2 by double enzyme digestion M: Marker 15000; 1: pET-28a(+)- LIS1 digested by double enzyme EcoRⅠand Hind Ⅲ; 2: pET-28a(+)-LIS1 linear plasmid; 3: pET-28a(+)- LIS2 digested by double enzyme NdeⅠ and EcoRⅠ; 4: pET-28a(+)-LIS2 linear plasmid"

Fig.11

Prokaryotic expression analysis of LIS1 and LIS2 recombinant proteins M: ProteinRuler®Ⅰ, the same below; 1: transetta with pET-28a(+) uninduced; 2: transetta with pET-28a(+) induced by IPTG; 3: transetta with pET-28a(+)-LIS2 before induction; 4: transetta with pET-28a(+)-LIS2 uninduced; 5: transetta with pET-28a(+)-LIS2 by IPTG; 6: transetta with pET-28a(+) uninduced; 7: transetta with pET-28a(+) induced by IPTG; 8: transetta with pET-28a(+)-LIS1 before induction; 9: transetta with pET-28a(+)-LIS1 uninduced; 10: transetta with pET-28a(+)-LIS1 by IPTG"

Fig.12

Soluble identification of LIS1 and LIS2 recombinant proteins 1: protein samples before induction; 2: non-induced protein sample; 3: protein samples after induction; 4: soluble sample after induction; 5: insoluble sample after induction"

Fig.13

Recovery identification of LIS1 and LIS2 recombinant proteins 1: inclusion body dissolution solution; 2: dissolved protein fluid; 3: dissolved protein eluent 1; 4: dissolved protein eluent 2"

Fig.14

GC-MS detection of output products of LIS1 recombinant protein supplemented with substrate"

Fig.15

GC-MS detection of output products of LIS2 recombinant protein supplemented with substrate Linalool, monoterpene, 1,6-octadien-3-ol, 3,7-dimethyl-, molecular formula: C10H18O"