Crops ›› 2022, Vol. 38 ›› Issue (4): 227-235.doi: 10.16035/j.issn.1001-7283.2022.04.032

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Study on the Suitable Harvest Date of High Availability Upper Leaves of Flue-Cured Tobacco in Southwestern Guizhou

Liu Xinya1(), Chen Xiaolong2, Feng Yake2, Liu Yang3, Duan Weidong2, An Xueqiang2, Chen Fayuan3, Cao Xingbing3, Zhao Yuanyuan1, Shi Hongzhi1()   

  1. 1Tobacco College, Henan Agricultural University, Zhengzhou 450002, Henan, China
    2Henan China Tobacco Industry Co., Ltd., Zhengzhou 450016, Henan, China
    3Guizhou Tobacco Company Qianxinan Prefecture Company, Qianxinan 562400, Guizhou, China
  • Received:2021-08-10 Revised:2022-05-15 Online:2022-08-15 Published:2022-08-22
  • Contact: Shi Hongzhi E-mail:1451309686@qq.com;shihongzhi88@163.com

Abstract:

Yunyan 87 was used as the experimental material to find out the suitable harvest time of high-availability upper leaves of flue-cured tobacco in the Southwestern Guizhou Autonomous Prefecture tobacco areas and to determine the mature harvest standard that is most suitable for local quality improvement. The effects of the harvest date of the upper six leaves (harvested 4 days in advance, normal, and delayed 4, 8, and 12 days) on the appearance quality, chemical composition, economic properties, and sensory quality were studied. The results showed that, with the delay of harvest date, the maturity of tobacco leaves and the nicotine content increased while the SPAD value and sugar-alkali ratio decreased. Properly delayed harvesting could improve the quality of the upper six tobacco leaves, reduce leaf thickness, improve porosity, increase oil content, increase aroma, reduce irritation, good sensory quality, and high economic benefits. Through the simulated regression equation of economic characters and sensory evaluation, it was concluded that the yield value was the highest when the upper tobacco leaves were harvested after a delay of 3.03 days. The total score of the upper tobacco leaves was the highest when the harvesting was delayed by 6.95 days. Based on the comprehensive economic benefits and sensory quality, the upper tobacco leaves in the tobacco areas of Southwest Guizhou Autonomous Prefecture should be appropriately delayed for 5-7 days. The SPAD values of the upper three leaves were 10.36-12.79 and the SPAD values of the lower three leaves were 11.82-13.82. It was more appropriate to harvest the lower three leaves first and then the upper three leaves, and then weave rods and install Kang. At this time, high-quality upper tobacco leaves with good yield and quality and high availability could be obtained.

Key words: Southwestern Guizhou Autonomous Prefecture, Flue-cured tobacco, Harvest date, Upper six leaves, Maturity level, SPAD value, Quality

Table 1

Experimental design"

处理编号
Treatment mark
处理
Treatment
采收日期(月-日)
Harvest date (month-day)
A4 提前4d 08-21
CK 正常采收 08-25
D4 延迟4d 08-29
D8 延迟8d 09-02
D12 延迟12d 09-06

Fig.1

Main appearance characteristics of tobacco leaf during different harvesting dates"

Table 2

Effects of different harvest dates on main appearance characteristics of tobacco leaves"

部位
Position
处理
Treatment
叶面色调
Leaf tone
叶面落黄程度
Degree of leaf
yellowness (%)
主脉变白程度
Whitening degree
of main pulse (%)
支脉变白程度
Whitening degree
of carbond (%)
绒毛脱落程度
Degree of leaf hair
Shedding (%)
成熟斑
Mature
spot
上3片
Upper three leaves
A4 黄绿 30~39 30~39 0~19 30~39 较少
CK 绿黄 40~49 40~49 20~39 50~59 较少
D4 浅黄 80~89 60~69 50~59 70~79 稍有
D8 浅黄 90~99 90~99 60~79 80~89 稍多
D12 淡黄 90~99 90~99 80~99 90~99 较多
下3片
Lower three leaves
A4 绿黄 40~49 40~49 10~29 40~49 较少
CK 绿黄 50~59 60~69 30~49 50~59 稍有
D4 浅黄 80~89 80~89 50~59 70~79 稍多
D8 淡黄 90~99 90~99 60~79 90~99 较多
D12 淡黄 90~99 90~99 80~99 90~99 较多

Fig.2

SPAD value of tobacco leaf during different harvest dates"

Table 3

Effects of different harvest dates on main appearance quality of tobacco leaves"

部位
Position
处理
Treatment
颜色
Color
油分
Oil
疏松度
Fraction void
成熟度
Maturity
色度
Chrominance
身份
Identity
残伤面积
Residual injury area (%)
上3片
Upper three leaves
A4 柠檬黄 稍有 紧密 尚熟 10~15
CK 橘黄 稍密 尚熟 10~15
D4 橘黄 尚疏松 成熟 稍厚 15~20
D8 橘黄 尚疏松 成熟 中等 20~25
D12 橘黄 稍有 尚疏松 完熟 稍薄 25~30
下3片
Lower three leaves
A4 柠檬黄 稍有 稍密 尚熟 稍厚 10~15
CK 橘黄 稍密 成熟 稍厚 10~15
D4 橘黄 尚疏松 成熟 中等 15~20
D8 橘黄 尚疏松 成熟 中等 25~30
D12 橘黄 稍有 尚疏松 完熟 稍薄 30~35

Table 4

Effects of different harvest dates on economic characters of tobacco leaves"

部位
Position
处理
Treatment
产值(元/hm2
Output value (yuan/hm2)
产量
Yield (kg/hm2)
均价(元/kg)
Mean price (yuan/kg)
单叶重
Weight per leaf (g)
上等烟比例
First-class tobacco proportion (%)
上3片
Upper three leaves
A4 16 549.40d 796.23cd 20.78c 17.69cd 47.96c
CK 23 710.81b 965.75a 24.55b 21.46a 65.54b
D4 24 746.45a 901.53b 27.45a 20.03b 73.78a
D8 20 104.36c 824.63c 24.38b 18.33c 66.83b
D12 14 349.29e 745.65d 19.24d 16.57d 39.82d
下3片
Lower three leaves
A4 20 878.27d 874.35bc 23.88c 19.43b 49.70c
CK 24 920.84b 929.43ab 26.81b 20.65a 68.93a
D4 27 947.71a 960.30a 29.10a 21.34a 70.82a
D8 22 429.27c 843.84c 26.58b 18.75b 64.48b
D12 15 402.02e 781.43d 19.71d 17.37c 37.56d

Table 5

Effects of different harvest dates on physical properties of tobacco leaves"

部位
Position
处理
Treatment
叶长
Leaf length
(cm)
叶宽
Leaf width
(cm)
单叶叶面积
Single leaf
area (cm2)
叶片厚度
Leaf thicken
(mm)
叶质重
Leaf density
(g/m2)
含梗率
Midrib ratio
(%)
拉力
Tensile
strength (N)
平衡含水率
Equilibrium moisture
content (%)
上3片
Upper three leaves
A4 67.82ab 20.58b 36.33b 0.25a 83.06a 31.73a 2.58b 19.73a
CK 70.30a 22.92a 38.71a 0.23b 78.42b 28.64b 3.14a 19.45a
D4 67.20b 23.30a 37.93a 0.23b 75.69b 26.52c 2.68b 18.44a
D8 65.50b 21.17b 35.94b 0.22c 64.69c 21.90d 2.59b 18.46a
D12 65.18b 21.04b 35.75b 0.18d 63.62c 25.40c 2.06c 19.66a
下3片
Lower three leaves
A4 75.60a 23.42b 40.81ab 0.23a 76.23a 29.56a 2.74ab 19.56b
CK 77.00a 24.64a 42.09ab 0.22a 75.82a 28.20a 2.89a 22.16a
D4 78.14a 24.72a 42.53a 0.21b 66.75b 26.67b 2.88a 21.94a
D8 76.17a 23.57ab 41.10ab 0.18c 62.80bc 23.21c 2.64b 19.19b
D12 74.62a 23.19b 40.33b 0.18c 61.66c 24.33c 2.16c 19.75b

Fig.3

Effects of different harvest dates on leaf density of tobacco leaves"

Fig.4

Effects of different harvest dates on midrib ratio of tobacco leaves"

Table 6

Effects of different harvest dates on chemical composition contents of tobacco leaves"

部位
Position
处理
Treatment
淀粉
Starch
(%)
烟碱
Nicotine
(%)
总氮
Total
nitrogen (%)
总糖
Total sugar
(%)
还原糖
Reducing
sugar (%)

Potassium
(%)

Chlorine
(%)
蛋白质
Protein
(%)
糖碱比
Sugar-
nicotine ratio
氮碱比
Nitrogen-
nicotine ratio
钾氯比
K-Cl
ratio
上3片
Upper
three leaves
A4 2.65d 2.67d 2.81a 19.78c 15.80d 1.32b 0.22d 13.00a 7.41c 1.05a 6.00a
CK 3.76b 3.03c 2.59b 27.12a 25.95a 1.35b 0.27c 11.77b 8.95a 0.86b 4.92c
D4 5.31a 3.29b 2.51bc 28.04a 26.80a 1.54a 0.28c 10.31c 8.52b 0.76c 5.44b
D8 3.40c 3.73a 2.32d 24.18b 23.31b 1.60a 0.36b 11.53b 6.48d 0.62d 4.43d
D12 2.42e 3.82a 2.37cd 19.35c 17.38c 1.55a 0.38a 12.82a 5.06e 0.62d 4.06e
下3片
Lower
three leaves
A4 3.88c 2.84e 2.88a 25.21c 23.45b 1.28c 0.26d 9.94d 8.87a 1.01a 4.93ab
CK 4.65b 3.02d 2.41bc 26.67b 24.34b 1.26c 0.30c 10.71bc 8.82a 0.80b 4.24d
D4 4.85a 3.29c 2.33c 29.08a 26.86a 1.44b 0.32bc 10.51c 8.84a 0.71c 4.51c
D8 3.37d 3.67b 2.47b 23.76d 21.58c 1.72a 0.34ab 11.16b 6.47b 0.67d 5.03a
D12 2.04e 3.90a 2.39bc 24.68cd 23.22b 1.72a 0.36a 13.15a 6.32b 0.61e 4.74b

Table 7

Effects of different harvest dates on aroma components of tobacco leaves μg/g"

香气物质
Aroma material
上3片Upper three leaves 下3片Lower three leaves
A4 CK D4 D8 D12 A4 CK D4 D8 D12
糠醛Furfural 19.76 22.55 23.55 25.53 29.89 20.98 21.48 21.81 24.33 24.13
糠醇Furfuralcohol 0.00 1.61 1.20 0.80 1.65 0.00 1.03 1.63 0.85 0.78
2-乙酰基吡咯2-acetylpyrrole 0.33 1.17 0.25 0.61 0.38 0.20 0.56 0.30 0.51 0.31
2-乙酰基呋喃2-acetylfuran 1.07 1.04 0.53 0.88 0.96 0.66 0.88 0.89 0.91 0.32
5-甲基糠醛5-methylfurfural 2.27 1.52 1.08 1.35 1.30 0.93 1.16 1.17 1.29 1.57
3,4-二甲基-2,5-呋喃二酮
3,4-isopropyl-2,5-furandione
1.21 1.42 1.17 1.13 1.96 0.84 1.69 1.66 1.54 1.26
2,6-壬二烯醛2,6-octadienal 1.10 2.07 2.83 1.63 2.56 1.70 2.04 2.25 1.63 2.25
棕色化反应产物总量
Total browning product
25.74d 31.37bc 30.63c 31.93b 38.70a 25.31d 28.83c 29.72bc 31.07ab 30.61a
愈创木酚Guaiacol 2.37 2.75 2.23 2.23 1.75 1.86 2.09 2.15 2.03 2.17
芳樟醇Linalool 0.76 0.91 0.75 0.77 1.15 0.72 0.94 0.95 0.92 0.73
6-甲基-5-庚烯-2-酮
6-methyl-5-hepten-2-one
0.51 0.61 0.56 0.42 1.21 0.55 0.65 0.79 0.92 0.50
藏花醛Safranal 0.52 0.74 0.85 0.64 0.50 0.49 0.92 0.68 0.73 0.49
β-环柠檬醛 β-Cyclocitral 0.74 0.97 0.81 0.87 0.83 0.78 0.88 0.88 0.82 0.60
β-大马酮 β-damascenone 21.27 19.65 18.94 19.25 15.30 19.71 19.57 18.81 18.26 16.05
β-二氢大马酮 β-damascenone 13.07 14.19 15.25 17.39 11.39 12.20 11.63 12.20 17.22 12.98
香叶基丙酮Geranylacetone 1.61 3.48 2.51 2.49 2.54 1.27 2.23 2.38 2.22 2.53
二氢猕猴桃内酯Dihydroactinidiolide 2.14 2.73 3.08 3.53 4.98 1.80 2.75 3.19 3.49 3.26
巨豆三烯酮1 Megastigmatrienone-1 1.96 1.69 1.87 2.02 1.75 1.41 1.59 1.59 1.52 2.28
巨豆三烯酮2 Megastigmatrienone-2 7.67 6.56 7.04 7.66 6.60 4.90 5.40 6.00 5.95 9.72
巨豆三烯酮3 Megastigmatrienone-3 6.16 6.80 7.38 7.61 6.95 6.95 6.92 6.50 6.31 5.91
3-羟基-β-二氢大马酮
3-hydroxy-β-damascenone
2.55 3.62 4.16 4.24 6.57 3.04 4.15 4.86 4.51 4.90
巨豆三烯酮4 Megastigmatrienone-4 11.96 11.75 11.80 13.09 12.54 8.73 9.34 10.40 10.32 15.78
螺岩兰草酮Snailvetiverketone 9.21 5.93 11.69 10.73 31.85 9.60 10.52 14.45 14.20 13.08
法尼基丙酮
(5E,9E)-6,10,14-trimethylpentadeca-5,9,
13-trien-2-one
9.25 15.14 13.34 16.69 18.96 11.03 14.17 15.56 18.35 15.81
类胡萝卜素降解产物总量
Total various of carotenoids
91.76d 97.52c 102.26c 109.62b 124.87a 85.03d 93.76c 101.39b 107.79a 106.79a
苯甲醛Benzaldehyde 0.41 0.45 0.63 0.59 0.52 0.25 0.61 0.59 0.75 1.05
苯甲醇Benzalcohol 7.98 11.17 15.21 16.69 19.53 7.95 12.44 13.10 14.65 18.21
苯乙醇Phenethyl alcohol 6.23 5.67 3.01 4.25 5.62 2.54 5.33 4.36 4.11 4.55
苯乙醛Phenylacetaldehyde 1.96 2.07 3.87 4.30 4.58 0.70 2.36 2.16 2.64 3.20
苯丙氨酸降解产物总量
Total phenylalanine
16.57e 19.36d 22.72c 25.83b 30.25a 11.44d 20.74c 20.21c 22.14b 27.01a
茄酮Solanone 33.69d 34.05d 40.83c 47.57b 57.08a 29.60d 33.10c 36.91b 37.00b 41.08a
新植二烯Neophytadiene 598.82d 611.15d 675.84c 716.09b 839.38a 633.89c 646.95c 712.10b 742.42ab 753.22a
中性致香成分总量Total aroma 766.58d 793.45d 872.27c 931.03b 1090.28a 785.27b 823.39b 900.33a 940.42a 958.71a

Table 8

Effects of different harvest dates on sensory quality of tobacco leaves"

部位
Position
处理
Treatment
烟气质量
Flue gas quality
烟气丰富程度
Flue gas abundance
烟气满足感
Smoke satisfaction
燃烧性
Flammability
总分
Total
score
香气质
Aroma
quality
刺激性
Irritancy
杂气
Offensive
odor
香气量
Aroma
quantity
浓度
Concen-
tration
透发性
Transvita-
lization
劲头
Stiffness
余味
After
taste
上3片
Upper three
leaves
A4 6.3ab 6.2abc 6.1b 6.0c 6.0b 6.0c 5.6c 6.3a 7.5b 56.0c
CK 6.3ab 6.2abc 6.3ab 6.3b 6.3ab 6.2bc 5.8bc 6.4a 7.5b 57.3bc
D4 6.7a 6.5a 6.5a 7.0a 6.5a 6.5ab 6.1ab 6.6a 8.0a 60.4ab
D8 6.5ab 6.5ab 6.5a 7.0a 6.6a 6.6a 6.4a 6.5a 8.0a 60.6a
D12 6.2b 6.1ac 6.3ab 6.8a 6.4ab 6.6a 6.3a 6.3a 8.0a 59.0abc
下3片
Lower three
leaves
A4 6.3a 6.2ab 6.1bd 6.2c 6.0c 6.0b 5.8c 6.2b 7.5b 56.3ab
CK 6.4a 6.3ab 6.4ab 6.5bc 6.2bc 6.2ab 6.0bc 6.5ab 7.5b 58.0ab
D4 6.5a 6.5a 6.6a 6.9a 6.4ab 6.3ab 6.1bc 6.6a 8.0a 59.9a
D8 6.3a 6.3ab 6.5a 6.8ab 6.6a 6.5a 6.5a 6.5ab 8.0a 60.0a
D12 6.2a 6.1b 6.4abc 6.8ab 6.5ab 6.3ab 6.3ab 6.4ab 8.0a 59.0ab

Table 9

Comprehensive evaluation of sensory quality and output value of tobacco leaves at different harvest dates"

感官评价指标
Sensory evaluation index
部位
Position
回归方程
Regression equation
R2 最佳延迟采收时间
Optimal delay time for harvesting (d)
烟气品质总分
Total flue gas quality score
上3片 y =-0.0147x2+0.1354x+19.206 0.7572 4.61
下3片 y =-0.0125x2+0.1050x+19.200 0.8981 4.20
烟气丰富程度总分
Total flue gas richness score
上3片 y =-0.0152x2+0.2464x+19.103 0.9579 8.11
下3片 y =-0.0107x2+0.1807x+19.031 0.9807 8.44
烟气满足感总分
Total flue gas satisfaction score
上3片 y =-0.0067x2+0.1061x+12.357 0.9229 7.92
下3片 y =-0.0067x2+0.1011x+12.497 0.9520 7.54
总分Total score 上3片 y =-0.0388x2+0.5432x+58.351 0.8878 7.00
下3片 y =-0.0321x2+0.4421x+58.414 0.9711 6.89
产值(元/hm2
Output value (yuan/hm2)
上3片 y =-140.67x2+925.22x+22943 0.9656 3.29
下3片 y =-136.99x2+795.76x+25852 0.9654 2.78
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