Crops ›› 2023, Vol. 39 ›› Issue (2): 138-144.doi: 10.16035/j.issn.1001-7283.2023.02.020

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Effects of Exogenous Nitric Oxide and Hydrogen Sulfide on the Germination of Foxtail Millet Seeds

He Shuiling(), Zhao Xia, Wu Mingqi, Wang Dongsheng()   

  1. College of Life Sciences, Shanxi Normal University, Taiyuan 030032, Shanxi, China
  • Received:2021-09-07 Revised:2021-12-02 Online:2023-04-15 Published:2023-04-11

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Abstract:

In order to study the effects of nitric oxide (NO), hydrogen sulfide (H2S) and their interaction on seed germination, this experiment took millet seeds as the research object. The seeds were treated with different concentrations of sodium nitroprusside (SNP) and sodium hydrosulfide (NaHS), donors of NO and H2S, respectively, for different durations and cultured under different light conditions. The effects of NO and H2S on seed germination of foxtail millet were evaluated by six indicators including germination rate, germination potential, germination index, vitality index, root length and bud length. The results showed that low concentrations of NO and H2S promoted seed germination, while high concentration of NO and H2S inhibited seed germination. There was an interaction between NO and H2S during the germination of foxtail millet seeds.

Key words: Nitric oxide, Hydrogen sulfide, Seed germination, Foxtail millet

Fig.1

Effects of SNP treatments on seed germination of foxtail millet at different concentrations “*”and“**”indicate significant and extremely significant difference at the P < 0.05 and P < 0.01 levels, respectively, the same below"

Fig.2

Effects of NaHS treatments on seed germination of foxtail millet at different concentrations"

Table 1

Effects of 0.010mmol/L SNP+0.100mmol/L NaHS treatment on seed germination of foxtail millet"

指标Index 处理Treatment 对照组CK SNP 0.010mmol/L NaHS 0.100mmol/L SNP 0.010+NaHS 0.100
发芽率Germination rate (%) T1 82.2±11.7a 84.4±3.8a 85.6±3.2a 72.2±3.1a
T2 75.6±11.1a 81.1±6.9a 73.3±1.7a 78.9±2.1a
T3 80.0±3.3a 84.4±7.7a 73.3±3.6a 77.8±0.6a
发芽势Germination potential (%) T1 47.8±11.7ab 44.4±12.6ab 61.1±3.8a 26.7±2.6b
T2 57.8±9.6a 62.2±9.6a 56.7±3.5a 52.2±0.6a
T3 58.9±5.1a 73.3±8.8a 61.1±1.5a 64.4±3.2a
发芽指数Germination index T1 23.8±2.6ab 22.6±1.1ab 26.9±4.5a 17.7±3.4b
T2 26.2±4.2a 27.7±1.9a 26.3±4.1a 25.8±2.3a
T3 26.3±1.6a 30.7±2.0a 25.9±3.7a 26.5±2.0a
活力指数Vitality index T1 87.6±33.5a 105.0±24.2a 104.4±23.2a 64.6±11.6a
T2 58.6±29.4a 85.3±15.1a 80.8±25.2a 80.3±6.3a
T3 75.4±10.0b 95.0±7.4a 78.0±11.7ab 79.5±7.3ab
根长Root length (cm) T1 3.7±1.7a 4.7±2.0a 3.9±0.4a 3.8±1.2a
T2 3.0±1.4a 3.1±1.5a 3.2±1.7a 3.1±0.1a
T3 2.9±1.0a 3.1±1.0a 3.0±0.8a 3.0±0.1a
芽长Bud length (cm) T1 1.5±0.5a 1.3±0.5ab 1.3±0.6ab 1.3±0.2b
T2 1.4±0.3a 1.1±0.4b 1.1±0.3b 1.3±0.2a
T3 1.6±0.9b 2.2±1.0a 1.9±0.9ab 2.0±1.0ab

Table 2

Effects of 0.010mmol/L SNP+0.400mmol/L NaHS treatment on seed germination of foxtail millet"

指标Index 处理Treatment 对照组CK SNP 0.010mmol/L NaHS 0.400mmol/L SNP 0.010+NaHS 0.400
发芽率Germination rate (%) T1 82.2±11.7a 84.4±3.8a 81.1±2.3a 86.7±1.7a
T2 75.6±11.1a 81.1±6.9a 80.0±1.7a 78.9±2.9a
T3 80.0±3.3ab 84.4±7.7a 63.3±2.6b 70.0±4.0ab
发芽势Germination potential (%) T1 47.8±11.7a 44.4±12.6a 38.9±6.8a 55.6±5.7a
T2 57.8±9.6a 62.2±9.6a 67.8±6.4a 60.0±1.0a
T3 58.9±5.1ab 73.3±8.8a 51.1±2.5b 52.2±3.1b
发芽指数Germination index T1 23.8±2.6a 22.6±1.1a 21.7±5.8a 24.7±3.2a
T2 26.2±4.2a 27.7±1.9a 29.8±5.5a 25.6±3.5a
T3 26.3±1.6ab 30.7±2.0a 22.1±2.5b 22.3±3.9b
活力指数Vitality index T1 87.6±33.5ab 105.0±24.2a 51.5±11.1b 131.6±19.5a
T2 58.6±29.4ab 85.3±15.1a 57.9±5.9bc 49.1±8.6c
T3 75.4±10.0b 95.0±7.4a 47.1±8.2c 49.7±10.1c
根长Root length (cm) T1 3.7±1.7b 4.7±2.0a 2.6±1.3c 5.4±1.2a
T2 3.0±1.4a 3.1±1.5a 2.0±1.1b 1.9±0.1b
T3 2.9±1.0a 3.1±1.0a 2.1±0.9b 2.2±0.1b
芽长Bud length (cm) T1 1.5±0.5a 1.7±0.5b 1.2±0.3b 1.4±0.2a
T2 1.4±0.3a 1.1±0.4b 1.0±0.4b 1.1±0.1b
T3 1.6±0.9b 2.2±1.0a 1.2±0.8bc 1.2±0.3c

Table 3

Effects of 0.200mmol/L SNP+0.100mmol/L NaHS treatment on seed germination of foxtail millet"

指标Index 处理Treatment 对照组 SNP 0.200mmol/L NaHS 0.100mmol/L SNP 0.200+NaHS 0.100
发芽率Germination rate (%) T1 82.2±11.7a 47.8±10.7b 85.6±3.2a 66.7±2.6ab
T2 75.6±11.1a 76.0±13.9a 73.3±1.7a 68.9±1.5a
T3 80.0±3.3a 77.8±8.4a 73.3±3.6a 66.7±2.0a
发芽势Germination potential (%) T1 47.8±11.7a 4.0±5.0b 61.1±3.8a 8.9±2.3b
T2 57.8±9.6a 52.0±18.4a 56.7±3.5a 51.1±4.5a
T3 58.9±5.1a 68.0±10.1a 61.1±1.5a 58.9±2.1a
发芽指数Germination index T1 23.8±2.6a 7.1±2.8b 26.9±4.5a 12.0±3.4b
T2 26.2±4.2a 24.1±5.0a 26.3±4.1a 22.5±4.2a
T3 26.3±1.6a 27.3±2.9a 25.9±3.7a 23.2±2.1a
活力指数Vitality index T1 87.6±33.5a 2.7±1.7b 104.4±23.2a 18.4±1.3b
T2 58.6±29.4a 46.0±11.3a 80.8±25.2a 56.9±15.3a
T3 75.4±10.0a 55.6±2.8b 78.0±11.7a 53.3±11.4b
根长Root length (cm) T1 3.7±1.7a 0.4±0.2c 3.9±0.4a 1.6±0.7b
T2 3.0±1.4a 1.9±1.4b 3.2±1.7a 2.5±0.3ab
T3 2.9±1.0a 2.1±0.7b 3.0±0.8a 2.3±0.5b
芽长Bud length (cm) T1 1.5±0.5a 0.7±0.3c 1.3±0.6a 1.1±0.4b
T2 1.4±0.3a 0.8±0.3c 1.1±0.3b 1.1±0.3b
T3 1.6±0.9a 1.2±0.6b 1.9±0.9a 1.8±1.0a
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