Crops ›› 2022, Vol. 38 ›› Issue (2): 211-214.doi: 10.16035/j.issn.1001-7283.2022.02.029

Previous Articles     Next Articles

Effects of Storage Time on Seed Germination and Physiological Characteristics of Tobacco Variety Honghuadajinyuan

Lu Jun(), Qian Yu, Yang Liu, Wang Yong, Chen Yulan   

  1. Liangshan Branch, Sichuan Tobacco Company, Xichang 615000, Sichuan, China
  • Received:2021-09-06 Revised:2021-10-20 Online:2022-04-15 Published:2022-04-24

RichHTML

4

PDF (PC)

138

Abstract:

Changes of germination, physiological and biochemical characteristics of tobacco seeds at different storage time were studied to provide references for tobacco germplasm conservation and field production. The seeds of tobacco variety ‘Honghuadajinyuan’ stored at room temperature for 0-12 months were used as materials to study the changes of germination characteristics, antioxidant enzyme activities, osmotic regulation substances (proline, soluble sugar), malondialdehyde (MDA) and relative conductivity during storage. The results showed that the vigor indexes, germination potential, germination rates and germination indexes of seeds increased first and then decreased with the extension of storage time. The highest germination rate was 93.21% after storage for six months, and the seeds basically lost germination activity after storage for 12 months. With the extension of storage time, superoxide dismutase, peroxidase and catalase activities, proline and soluble sugar contents decreased, MDA content and relative conductivity increased. This indicated that during the aging process of tobacco seeds, the germination ability of seeds increased first and then decreased, the activities of antioxidant enzymes decreased as a whole, the consumption of osmotic regulation substances decreased, and the damage of cell membrane intensified. In the actual production, seeds stored at room temperature for six months are recommended.

Key words: Tobacco, Seed germination, Storage time, Physiological and biochemical characteristics

Table 1

Effects of storage time on seed germination indexes in tobacco"

贮藏时间（月）
Storage time（month)		 发芽率
Percentage of germination (%)		 发芽势
Germinating potential (%)		 发芽指数
Germination index		 活力指数
Vitality index
0 61.27±3.71c 53.71±3.55c 11.63±0.88b 1.72±0.55b
3 78.45±4.23b 71.47±3.68b 12.11±1.23b 1.92±0.43b
6 93.21±5.15a 91.63±4.96a 22.75±1.11a 3.57±0.76a
9 38.65±2.98d 17.53±1.55d 9.94±0.82b 1.85±0.58b
12 3.85±0.17e 0.00±0.00e 0.42±0.03c 0.23±0.01c

Table 2

Effects of storage time on osmotic regulation substances in tobacco"

贮藏时间（月）
Storage time
(month)		 脯氨酸含量
Proline content
(μg/g)		 可溶性糖含量
Soluble sugar content
(mg/g)
0 8.11±0.24a 14.78±0.66a
3 7.64±0.31a 11.89±0.56b
6 5.17±0.24b 11.36±0.44b
9 4.65±0.23b 10.23±0.37b
12 4.72±0.15b 10.76±0.58b

Fig.1

Effects of storage time on SOD activity in tobacco Different lowercase letters indicate significant difference between different storage times at the 0.05 level, the same below"

Fig.2

Effects of storage time on POD activity in tobacco"

Fig.3

Effects of storage time on CAT activity in tobacco"

Table 3

Effects of storage time on relative conductivity and MDA content in tobacco"

贮藏时间（月）
Storage time
(month)		 相对电导率
Relative conductivity
(%)		 MDA含量
MDA content
(nmol/g)
0 23.54±4.61c 2.51±0.36b
3 27.87±5.34c 2.45±0.41b
6 28.63±3.56c 2.67±0.37b
9 41.16±2.98b 2.93±0.57b
12 55.94±3.77a 4.15±0.51a
[1] 赵凯, 李珊珊, 马倩, 等. 花生种子自然老化对品质及发芽的影响. 核农学报, 2021, 35(2):490-497.
[2] 吴汉花, 蒋芳玲, 曹雪, 等. 不同老化程度的不结球白菜种子活力指标变化及其相关分析. 西北植物学报, 2012, 32(8):1606-1614.
[3] 卢军, 娄伟, 罗琳, 等. 低温和长时间贮藏对烟草种子萌发和幼苗渗透调节物质的影响. 南方农业学报, 2014, 45(11):1943-1946.
[4] 许美玲. 烟草种子超干燥贮藏及相关技术研究. 农业工程学报, 2005, 21(12):156-162.
[5] 陈建国, 朱列书, 黄智, 等. 不同贮藏年限烟草种子活力研究. 作物研究, 2015, 29(1):32-36.
[6] 许美玲. 烟草种子的老化及发芽规律研究. 种子, 2006, 25(9):9-13.
[7] 张志良. 植物生理实验指导. 北京: 高等教育出版社, 1990.
[8] 李合生. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000.
[9] 余波, 杜尚广, 罗丽萍. 种子活力测定方法. 中国科学:生命科学, 2015, 45(7):709-713.
[10] 唐嘉, 毛春力, 黄琪, 等. 不同贮藏年限老芒麦种胚线粒体抗氧化功能的变化规律. 草地学报, 2020, 28(5):1226-1232.
[11] 秦源源, 张桓溥, 罗富成, 等. 储藏年限对纳罗克非洲狗尾草种子活力及其营养物质含量的影响. 云南农业大学学报(自然科学), 2018, 33(3):480-484.
[12] 许美玲, 刘慧慧. 种质库保存的烟草种子活力和出苗率及其关系. 云南农业大学学报(自然科学), 2016, 31(3):469-477.
[13] 江福英, 李延, 翁伯琦. 植物低温胁迫及其抗性生理. 福建农业学报, 2002, 17(3):190-195.
[14] 刘逸童, 张嘉伟, 孙萌, 等. 低温贮藏对水稻种子活力及种胚显微结构的影响. 分子植物育种, 2021, 19(7):2330-2337.
[15] 马向丽, 何超, 罗富成, 等. 水引发对不同贮藏年限纳罗克非洲狗尾草种子活力的影响. 中国草地学报, 2017, 39(5):16-23.
[16] 李西, 吴亚娇, 孙凌霞. 铅胁迫对三种暖季型草坪草生长和生理特性的影响. 草业学报, 2014, 23(4):171-180.
[17] 列淦文, 叶龙华, 薛摇立. 臭氧胁迫对植物主要生理功能的影响. 生态学报, 2014, 34(2):294-306.
[18] 张旭, 聂刚, 黄琳凯, 等. 低温贮藏不同年限的鸭茅种子活力变化. 草业科学, 2018, 35(10):2450-2456.
[19] 刘明久, 王铁固, 陈士林, 等. 玉米种子人工老化过程中生理特性与种子活力的变化. 核农学报, 2008, 22(4):510-513.
[1] Yun Fei, Ren Tianbao, Yin Quanyu, Jin Shuangzhen, Zheng Cong, Jin Lei, Li Jingjing, Liu Guoshun, Yang Xitian. Effects of Calcium by Foliage Spraying on Photosynthesis Physiology Characteristics of Flue-Cured Tobacco under Light Stress [J]. Crops, 2022, 38(2): 143-152.
[2] Tan Zhiyong, Shen Xiaoxiong, Liu Jie, Tan Daxin, Bai Bin, Zhou Xinghua, Zhao Hui. Spatial Variation Characteristics of Main Nutrients in Tobacco Planting Soil in Tongren City, Guizhou Province [J]. Crops, 2022, 38(2): 189-194.
[3] Meng Fan, Luo Jianxin, Cai Ye, Yu Ying, Yang Lei, Zhou Wanchun. Effects of Soil Available Phosphorus on Tobacco Growth and Dry Matter Accumulation and Distribution [J]. Crops, 2022, 38(2): 203-210.
[4] Zhang Yudan, Ma Xiaohan, Li Junling, Xu Zicheng, Jia Wei, Shi Qiuhuan. Inhibitory Effect of Chlorogenic Acid on Phytophthora nicotiana and Its Control Effect on Tobacco Black Shank Disease [J]. Crops, 2022, 38(2): 230-236.
[5] Zhang Siwei, Li Jin’ao, Liu Boyuan, Jiang Yuchen, Zhong Qiu, Lei Yunkang, Zhang Mingyue, Zhao Mingqin. Effects of Topping Method on Nitrogen Accumulation and Quality of Cigar Tobacco Leaves [J]. Crops, 2022, 38(1): 184-189.
[6] Gu Yuchao, Yang Yide, Yan Min, Liu Yong, Yang Jian, Xiang Jinyou, Luo Zhushi, Li Linqiu, Jing Yanqiu, Yang Yang. Effects of GA3 and 6-BA on Agronomic Traits and Chemical Components of Flue Cured Tobacco after Topping [J]. Crops, 2021, 37(6): 171-176.
[7] Li Maosen, Gao Weikai, Ren Tianbao, Jiang Shixiang, He Xiaoya, Luo Leqin, Yun Fei, Ke Xiaoting. Analysis of Bacterial Community and Influencing Factors in Tobacco Soil at Different Altitudes in Zunyi [J]. Crops, 2021, 37(6): 193-198.
[8] Li An, Shu Jianhong, Liu Xiaoxia, Meng Zhengbing, Wang Xiaoli, Zhao Degang. Effects of Bacillus subtilis on Drought Resistance and Physiological Indexes of Maize Seeds under Drought Stress [J]. Crops, 2021, 37(6): 217-223.
[9] Wang Chuliang, Song Wenfeng, Guan Luohao, Xie Jin, Huang Hao, Li Wangyang, Wang Wei. Effects of Film Mulching Method and Transplanting Seedling Age on Yield and Quality of Flue-Cured Tobacco in Honghe [J]. Crops, 2021, 37(6): 95-100.
[10] Zhou Qiyun, Zheng Chongyi, Jing Yongfeng, Liu Yongjun, Peng Shuguang, Chen Tao, Liu Zhixuan, Hu Ruiwen, Zhou Qingming, Li Juan. Study on Soil Organic Matter Content and Its Relationship with Nitrogen, Phosphorus and Potassium in Different Soil Layers of Rice-Growing Tobacco Areas in Southern Hunan [J]. Crops, 2021, 37(5): 114-119.
[11] Liu Wenlong, Ning Shanghui, Cao Mingfeng, Zhu Li, Gao Yuzhen, Zhang Xuewei, Wen Zixiang, Jiang Baodi, Jing Yanqiu, Deng Yong. Correlation Analysis of Soil Micronutrient and Chemical Components of Tobacco Leaves in Taoyuan County [J]. Crops, 2021, 37(5): 176-180.
[12] Zhang Jiuquan, Yu Xiangwen, Ling Aifen, Wang Yong, Li Leilei, Dong Jianxin. Optimal Specification of Tobacco Seedling Tray for Small Seedling Transplanting under Plastic Film [J]. Crops, 2021, 37(4): 123-129.
[13] Zhang Jiawen, Lu Shaohao, Zhao Mingqin, Zhong Qiu, Wang Jun, Yi Kai, Xiang Huan. Effects of Nitrogen Application Rates on Carbon and Nitrogen Metabolism and Quality of Cigar Leaves in Sichuan [J]. Crops, 2021, 37(4): 159-165.
[14] Yan Feng, Li Qingquan, Dong Yang, Ji Shengdong, Han Yehui, Yu Yunkai, Wang Lida, Zhao Suo. Effects of 60Co-γ Radiation on the Seed Germination and Seedling Growth of Broomcorn Millet [J]. Crops, 2021, 37(4): 202-205.
[15] Wang Kun, Wei Yuewei, Ji Xiaoming, Yun Fei, Zou Kai, Long Zhun. Effects of Combined Application of Biochar-Based Fertilizer and Trichoderma harzianum on the Qualities of Flue-Cured Tobacco and Tobacco-Growing Soil [J]. Crops, 2021, 37(3): 106-113.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!