Crops ›› 2024, Vol. 40 ›› Issue (4): 96-102.doi: 10.16035/j.issn.1001-7283.2024.04.012

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Effects of Simulated Seawater Stress on Wheat Germination

Li Han1(), Zhao Yuxue1, Zhou Xiaoke1, Li Yun2, Guo Zhenqing1, Wang Jian1, Han Yucui1(), Lin Xiaohu1()   

  1. 1College of Agronomy and Biotechnology, Hebei Normal University of Science and Technology / Hebei Key Laboratory of Crop Stress Biology, Qinhuangdao 066004, Hebei, China
    2Research Center of Rural Vitalization, Hebei Normal University of Science and Technology, Qinhuangdao 066004, Hebei, China
  • Received:2023-06-19 Revised:2023-07-17 Online:2024-08-15 Published:2024-08-14

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Abstract:

In order to clarify the effects of saline-alkali (simulated seawater) stress on germination related traits of different wheat varieties, four wheat varieties were used as materials, simulated seawater solutions with 0.0 (CK), 0.2, 0.4, 0.6, 0.8 and 1.0 times standard salinity were used for stress, and the ideal screening concentration of simulated seawater stress at the germination stage was determined by analyzing the differences in salinity tolerance during germination of four wheat varieties. The results showed that Jinnong 7 and Zhongmai 998 showed much less inhibition in the relative germination potential and relative germination rate compared to Tainong 18 and Jimai 229 under saline-alkali stress, and the inhibition of the four varieties was more serious with the increase of saline-alkali concentration. The relative shoot length, relative maximum root length and relative shoot fresh weight of the four wheat varieties decreased significantly under stress compared to CK, and the inhibition was more serious with the increase of saline-alkali concentration, among which Zhongmai 998 was the least inhibited, and Jimai 229 was the most inhibited. When the concentration was increased to 0.8 times, all four wheat varieties could not germinate. The effects of saline-alkali stress on relative root fresh weight of four varieties were significantly different, and Jinnong 7 was the least affected. Compared with CK, the relative root-shoot ratio of Jinnong 7 and Jimai 229 increased significantly under saline-alkali stress, and under 0.4 times concentration, Jinnong 7 (250.63) was higher than those of other varieties. Comprehensive analysis showed that Jinnong 7 and Zhongmai 998 were saline-alkali tolerant varieties, Tainong 18 was intermediate variety, and Jimai 229 was saline-alkali sensitive varieties, the 0.4 times concentration was the suitable concentration for screening wheat saline-alkali tolerance during germination stage. The correlations between relative root-shoot ratio and relative germination potential, relative shoot length, relative maximum root length, and relative shoot fresh weight were not significant, but there were significant or extremely significant correlations among other indexes of seven saline-alkali related characteristics at wheat germination stage.

Key words: Wheat, Germination period, Saline-alkali stress, Simulated seawater

Table 1

Formula for standard salinity artificial seawater"

成分
Constituent		 分子量
Molecular
weight		 溶液浓度
Solution
concentration (g/L)
氯化钠NaCl 58.44 23.926
硫酸钠Na2SO4 142.04 4.008
氯化钾KCl 74.56 0.677
碳酸氢钠NaHCO3 84.00 0.196
溴化钾KBr 119.01 0.098
硼酸H3BO3 61.83 0.026
氟化钠NaF 41.99 0.003
六水氯化镁MgCl2·6H2O 203.33 0.053
二水氯化钙CaCl2·2H2O 147.03 0.010
六水氯化锶SrCl2·6H2O 266.64 0.001
纯水H2O 加纯水溶解各种盐，室温定容至1 L

Table 2

Relative germination potential of different wheat varieties under salt-alkali stress %"

品种
Variety		 浓度Concentration
0.0 (CK) 0.2 0.4 0.6 0.8 1.0
津农7号Jinnong 7 100.00±0.00Aa 100.00±0.00Aa 93.37±5.77Aa 77.77±5.08Ba 67.77±5.08Ca 54.43±5.10Da
中麦998 Zhongmai 998 100.00±0.00Aa 100.00±0.00Aa 92.23±5.08Aa 80.00±3.30Ba 75.53±10.72Ba 51.13±5.10Ca
泰农18 Tainong 18 100.00±0.00Aa 88.27±7.67Bb 87.33±6.69Ba 75.87±8.17Ca 52.10±6.71Db 17.43±3.23Eb
济麦229 Jimai 229 100.00±0.00Aa 72.30±0.00Bc 57.53±17.48Cb 17.27±0.60Db 13.77±3.23DEc 0.00±0.00Ec

Table 3

Relative germination rate of different wheat varieties under salt-alkali stress %"

品种
Variety		 浓度Concentration
0.0 (CK) 0.2 0.4 0.6 0.8 1.0
津农7号Jinnong 7 100.00±0.00Aa 100.00±0.00Aa 97.78±3.85Aa 86.67±0.00Ba 75.56± 5.09Ca 66.66±5.77Da
中麦998 Zhongmai 998 100.00±0.00Aa 100.00±0.00Aa 97.78±1.92Aa 85.56±1.93Ba 77.78±8.39Ba 55.55±6.94Ca
泰农18 Tainong 18 100.00±0.00Aa 93.06±3.64Ab 92.07±4.96Aa 77.11±4.54Bb 74.69±4.11Ba 37.69±13.74Cb
济麦229 Jimai 229 100.00±0.00Aa 87.30±2.35Ac 68.08±20.54Bb 24.12±3.15Cc 12.57±3.55CDb 4.68±4.05Dc

Table 4

Relative shoot length of different wheat varieties under salt-alkali stress %"

品种
Variety		 浓度Concentration
0.0 (CK) 0.2 0.4 0.6
津农7号Jinnong 7 100.00±0.00Aa 51.25±7.52Ba 15.47±1.21Cb 5.09±0.22Db
中麦998 Zhongmai 998 100.00±0.00Aa 73.97±5.49Ba 48.96±5.99Ca 8.15±1.05Da
泰农18 Tainong 18 100.00±0.00Aa 60.24±12.28Ba 19.27±3.05Cb 4.43±1.03Db
济麦229 Jimai 229 100.00±0.00Aa 69.73±19.01Ba 4.76±0.61Cc 3.32±0.92Cb

Table 5

Relative maximum root length of different wheat varieties under salt-alkali stress %"

品种
Variety		 浓度Concentration
0.0 (CK) 0.2 0.4 0.6
津农7号Jinnong 7 100.00±0.00Aa 27.18±4.22Bb 6.95±0.54Cb 3.50±0.78Cb
中麦998 Zhongmai 998 100.00±0.00Aa 52.78±7.29Ba 22.77±4.35Ca 10.44±2.08Da
泰农18 Tainong 18 100.00±0.00Aa 44.31±11.30Ba 11.43±4.62Cb 4.08±0.16Cb
济麦229 Jimai 229 100.00±0.00Aa 41.69±7.64Bab 7.31±0.53Cb 3.21±0.18Cb

Table 6

Relative shoot fresh weight of different wheat varieties under salt-alkali stress %"

品种
Variety		 浓度Concentration
0.0 (CK) 0.2 0.4 0.6
津农7号Jinnong 7 100.00±0.00Aa 57.72±6.81Ba 37.55±8.42Ca 7.30±0.14Db
中麦998 Zhongmai 998 100.00±0.00Aa 67.31±4.17Ba 45.48±3.22Ca 10.01±1.38Da
泰农18 Tainong 18 100.00±0.00Aa 57.83±11.66Ba 21.53±3.97Cb 5.92±0.81Db
济麦229 Jimai 229 100.00±0.00Aa 71.22±13.14Ba 8.18±1.28Cc 5.10±2.90Cc

Table 7

Relative root fresh weight of different wheat varieties under salt-alkali stress %"

品种
Variety		 浓度Concentration
0.0 (CK) 0.2 0.4 0.6
津农7号Jinnong 7 100.00±0.00Aa 100.00±21.89Aa 94.76±27.82Aa 18.38±2.58Ba
中麦998 Zhongmai 998 100.00±0.00Aa 77.80±9.49Ba 61.99±19.03Bab 11.99±2.36Cb
泰农18 Tainong 18 100.00±0.00Aa 96.27±13.26Aa 24.75±21.06Bbc 10.24±3.96Bb
济麦229 Jimai 229 100.00±0.00Aa 99.43±15.61Aa 19.53±5.55Bc 7.46±1.80Bb

Table 8

Relative root-shoot ratio of different wheat varieties under saline-alkali stress %"

品种
Variety		 浓度Concentration
0.0 (CK) 0.2 0.4 0.6
津农7号Jinnong 7 100.00±0.00Ca 172.59±24.36Ba 250.63±28.94Aa 251.98±38.65Aa
中麦998 Zhongmai 998 100.00±0.00Aa 116.45±21.16Ab 138.54±51.03Aab 122.16±32.13Ac
泰农18 Tainong 18 100.00±0.00Aa 171.50±44.96Aa 120.47±107.75Ab 171.82±54.25Abc
济麦229 Jimai 229 100.00±0.00Da 140.38±9.32Cab 235.32±33.39Bab 197.97±17.09Aab

Table 9

Correlation analysis of wheat characteristics at germination stage under saline-alkali stress"

性状
Trait		 相对发芽势
Relative germination
potential		 相对发芽率
Relative
germination rate		 相对芽长
Relative shoot
length		 相对最大根长
Relative maximum
root length		 相对芽鲜重
Relative shoot
fresh weight		 相对根鲜重
Relative root
fresh weight		 相对根冠比
Relative root-
shoot ratio
相对发芽势
Relative germination potential		 1.000
相对发芽率
Relative germination rate		 0.652**
 1.000
相对芽长Relative shoot length 0.489* 0.629** 1.000
相对最大根长
Relative maximum root length		 0.449*
 0.562**
 0.969**
 1.000
相对芽鲜重
Relative shoot fresh weight		 0.497*
 0.656**
 0.992**
 0.959**
 1.000
相对根鲜重
Relative root fresh weight		 0.494*
 0.694**
 0.900**
 0.814**
 0.938**
 1.000
相对根冠比
Relative root-shoot ratio		 0.067
 0.423*
 0.204
 0.134
 0.265
 0.447*
 1.000
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