Crops ›› 2024, Vol. 40 ›› Issue (4): 90-95.doi: 10.16035/j.issn.1001-7283.2024.04.011

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Identification of Disease Resistance-Related Genes of Wheat Cultivars (Lines) in Southeastern Shanxi by KASP Assays

Yan Jinlong(), Zhang Dongxu, Feng Liyun, Wu Zhiyuan, Li Yijuan, Zhang Junling()   

  1. Millet Research Institute, Shanxi Agricultural University, Changzhi 046011, Shanxi, China
  • Received:2023-04-10 Revised:2023-06-19 Online:2024-08-15 Published:2024-08-14

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Abstract:

In order to clarify the distribution of resistance genes in wheat varieties (lines) in southeastern Shanxi, seven pairs of KASP markers relating stripe rust, leaf rust and powdery mildew resistance genes were used for gene detection of 47 varieties (lines) in southeastern Shanxi. The results showed that in all 47 tested materials, 40 materials contained the leaf rust resistance gene Lr14a, accounting for 85.1% of the total materials; Leaf rust resistance gene Lr34 was not detected; The leaf rust resistance gene Lr46 was detected in four materials, accounting for 8.5% of the total materials; There were 12 materials containing leaf rust resistance gene Lr68, accounting for 25.5% of the total materials; No stripe rust resistance gene Yr15 was detected; Only one material contained powdery mildew resistance gene Pm21. Among the six resistance genes detected, only one material (16-7885) contained three genes (Lr14a, Lr46, Lr68), and all of them were leaf rust resistance genes. There were 14 materials containing two resistance genes, in which 11 materials (Chang 4853, Chang 5553, 12-9499, 16-7381, 16-7527, 17-9627, 17-6665, 17-7103, 17-6925, 17-6755, 17-9467) contained two leaf rust resistance genes (Lr14a and Lr68), two materials (Chang 6359, Chang 6990) contained two leaf rust resistance genes (Lr14a and Lr46), one material (17-6891) contained powdery mildew resistance gene Pm21 and leaf rust resistance gene Lr46.

Key words: Southeastern Shanxi, Wheat, Disease-resistance, KASP markers, Breeding

Table 1

Details of related genes and markers"

基因
Gene		 标记
Marker		 FAM引物
FAM primer		 HEX引物
HEX primer		 通用引物
Common primer
Lr14a
 ubw14
 CTACACTAGTACTACTTTGAGACAATTTTTT ACACTAGTACTACTTTGAGACAATTTTAA AACAAACTCCAGTGTAAACACCACAGTTT
Lr34
 Lr34_TCCIND
 GGTATGCCATTTAACATAATCATGAA
 GGTATGCCATTTAACATAATCATGAT
 TACTATATGGGAGCATTATTTTTTTCC
Lr46 Lr46g AGCGGATCTGAGGTTTCCCTCC AGCGGATCTGAGGTTTCCCTCT CACCAGAGCCGACAACGGCAA
Lr68 Lr68-2 CGTGTCTTGGACCTGAGCAAT CGTGTCTTGGACCTGAGCAAC TGACCTGAGTCCCGTCAAGA
Yr15-1
 Yr15-R5
 AGTCAACTTGGATTACACTGAAGTT
 AGTCAACTTGGATTACACTGAAGTC
 AGATATCACACTGAACATACTGATGAG
Yr15-2 Yr15-R8 CAGATCCCCGGTTCTCTCAAG CAGATCCCCGGTTCTCTCAAA CCCCCAAATGATCGAGAATA
Pm21 Pm21_SNP GTGCTTTCCTGTGTGAACAGAGAT GTGGTTTCCTGTGTGAACACAGAA CACGACTCCACCCAGCAGC

Table 2

Detection of disease-resistant KASP markers in 47 materials from southeastern Shanxi"

品种（系）Variety (line) 组合Combination Lr14a Lr34 Lr46 Lr68 Yr15 Pm21
晋麦63 Jinmai 63 旱83-3227/长治2017 + － － － － －
长6878 Chang 6878 临旱5175/晋麦63号 + － － － － －
长4640 Chang 4640 长治5613/晋麦63号 + － － － － －
长6154 Chang 6154 晋麦63号/运8337-17-5-2-1 + － － － － －
长6452 Chang 6452 晋麦63号/长治4025 + － － ? － －
长6359 Chang 6359 82230-6/94-5383 + － + － － －
长4738 Chang 4738 82230-6/94-5383 － － － － － －
长5222 Chang 5222 98-6619/98-5764 + － － － － －
长8744 Chang 8744 晋麦62号/98-6160 + － － － － －
长4853 Chang 4853 CA9660/97-6828 + － － + － －
长6990 Chang 6990 03-6564/晋太170 + － + － － －
长7080 Chang 7080 03-6838/核丰4号 + － － － － －
长6794 Chang 6794 京农CR188/长6452 + － － － － －
长5804 Chang 5804 京农CR188/长6452 + － － － － －
长6388 Chang 6388 长8744/长4738 － － － － － －
长7170 Chang 7170 长6878/长4738 － － － － － －
长6213 Chang 6213 长6878/长4738 + － － － － －
长5638 Chang 5638 长治05-5981/运麦268 + － － － － －
长5553 Chang 5553 邯00-5030/长4738 + － － + － －
12-9499 邯00-5030/长6564 + － － + － －
14-7052 长4738/临旱7061//长4738 BC3F3 + － － － － －
15-6062 长 8744/长4738 + － － － － －
16-7228 长4738/临旱7061//长4738 BC3F3 － － － － － －
16-7381 运麦268/太原11406 + － － + － －
16-7425 洛旱6号/长6452 － － － － － －
16-7527 良星99/08-5430 + － － + － －
16-7117 长4738/krickaff//长4738 BC3F3 － － － － － －
16-7885 长6878/冀曲麦12号 + － + + － －
16-5332 长治05-5981/运麦268 + － － － － －
17-9627 运麦268/太原11406 + － － + － －
17-6441 11-6788/临旱006 + － － － － －
17-6505 11-6788/临旱006 + － － － － －
17-6655 10-9323/9623 + － － + － －
17-7103 05-5028/运麦268 + － － + － －
17-6803 长治05-5981/运麦268 + － － － － －
17-6913 长治05-5981/运麦268 + － － － － －
17-7019 长治05-5981/运麦268 + － － － － －
17-6741 长麦5765/长4738 + － － － － －
17-6925 藁8901/长8744 + － － + － －
17-6581 11-7227/兰考906 + － － － － －
17-7129 邯4589/长6452 + － － － － －
17-9915 邯4589/长6452 + － － － － －
17-6755 运麦298/长8744 + － － + － －
17-6891 长8744/长治03-6216 ? ? + － － +
17-9143 11-6790/太4906 + － － － － －
17-9401 046097/长6452 + － － － － －
17-9467 C26/长4738 + － － + － －

Fig.1

Proportion of different genes number in testing materials"

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