Crops ›› 2025, Vol. 41 ›› Issue (5): 165-170.doi: 10.16035/j.issn.1001-7283.2025.05.022

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Physiological Mechanism of Ammonium-Nitrate Mixed Nutrition Promoting Rice Growth under High-Temperature Stress

Peng Binfeng1,2(), Lu Chusheng1, Yin Yuanhong1, Zhu Feifei1,2, Ye Qunhuan1, Pan Junfeng1, Liu Yanzhuo1, Hu Xiangyu1, Hu Rui1, Li Meijuan1, Wang Xinyu1, Liang Kaiming1(), Fu Youqiang1()   

  1. 1 Rice Research Institute, Guangdong Academy of Agricultural Sciences / Guangdong Key Laboratory of Science and Technology in Rice / Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs /Guangdong Rice Engineering Laboratory, Guangzhou 510640, Guangdong, China
    2 Zhongkai University of Agriculture and Engineering, Guangzhou 510000, Guangdong, China
  • Received:2024-07-18 Revised:2024-09-15 Online:2025-10-15 Published:2025-10-21

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Abstract:

Taking rice varieties “Guanghui 751” and “Yuehesimiao” as experimental materials, different ammonium-nitrate mixed nutrition treatments (100:0, 75:25, 0:100) were set up to analyze the effects of different ammonium-nitrate mixed nutrition on rice biomass, root morphology, proline, malondialdehyde and antioxidant enzyme activities under high temperature stress conditions (average daytime temperature of 40-45 ℃ and average nighttime temperature of 30-35 ℃). The results showed that under high temperature stress, the biomass of rice in the 75:25 treatment was the largest, and compared with the 100:0 and 0:100 treatments, the shoot biomass was increased by 9.94%-16.59% and 22.01%-23.98%, respectively. Total root length, root surface area and root volume were increased with the increasing of nitrate nitrogen, but there was no significant difference in root diameter. Compared with the 100:0 treatment, the catalase (CAT), peroxidase (POD) and ascorbate peroxidase (APX) activities of Guanghui 751 under 75:25 treatment were increased by 250.00%, 60.42% and 207.00%, respectively, and the proline content was enhanced by 55.67%. The CAT and APX activities of Yuehesimiao were significantly increased, and the malondialdehyde content was decreased by 33.84%. These results indicated that 75:25 ammonium-nitrate ratio could alleviate the high temperature stress in rice by improving the antioxidant enzyme activity and affecting proline or malondialdehyde contents in rice.

Key words: Rice, High-temperature stress, Ammonium-nitrate mixed nutrition, Proline, Malondialdehyde

Fig.1

The average temperature during treatments"

Table 1

Dry matter of rice seedlings under different ammonium and nitrate mixed nutrition treatments mg/株mg/plant"

品种Variety 处理Treatment 根系生物量Root biomass 地上部生物量Shoot biomass 总生物量Total biomass
广恢751 Guanghui 751 100:0 43.53±6.99b 375.57±8.18b 419.10±8.59b
75:25 49.97±0.92b 437.87±5.83a 487.83±3.07a
0:100 65.00±5.96a 394.43±13.67b 459.43±11.02a
粤禾丝苗Yuehesimiao 100:0 49.27±2.85b 404.43±19.14b 453.70±16.68b
75:25 53.93±1.75a 444.63±24.27a 498.57±14.77a
0:100 58.00±2.01a 358.63±11.93b 416.63±8.02b
品种Variety ns ns ns
处理Treatment ** ** **
品种×处理Variety×Treatment * ** **

Table 2

Effects of different ammonium and nitrate mixed nutrition treatments on root morphology of rice"

品种
Variety		 处理
Treatment		 总根长
Total root length (cm)		 根直径
Root diameter (cm)		 根表面积
Root surface area (cm2)		 根体积
Root volume (cm3)
广恢751 Guanghui 751 100:0 214.08±40.66b 0.34±0.01a 22.68±3.81b 0.19±0.03b
75:25 248.76±13.10b 0.33±0.00a 25.89±1.26b 0.21±0.01b
0:100 341.99±20.94a 0.34±0.01a 37.01±2.92a 0.32±0.03a
粤禾丝苗Yuehesimiao 100:0 268.86±7.14b 0.32±0.01a 27.27±0.88b 0.22±0.01b
75:25 288.08±20.63ab 0.32±0.01a 29.15±2.48b 0.24±0.02b
0:100 322.52±22.69a 0.33±0.01a 33.55±2.45a 0.28±0.02a
品种Variety * ns ns ns
处理Treatment ** ** ** **
品种×处理Variety×Treatment * * * *

Fig.2

Effects of different ammonium and nitrate mixed nutrition treatments on Pro and MDA contents of rice seedlings Different lowercase letters indicate significant differences at the P < 0.05 level. The same below."

Fig.3

Effects of different ammonium and nitrate mixed nutrition treatments on antioxidant enzyme activities of rice leaves"

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