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作物杂志,2017, 第3期: 6–13 doi: 10.16035/j.issn.1001-7283.2017.03.002

• 专题综述 • 上一篇    下一篇

高油酸花生遗传育种研究进展

李拴柱1,宋江春1,王建玉1,张秀阁1,乔建礼1,刘宁2   

  1. 1 南阳市农业科学院,473000,河南南阳
    2 南阳市卧龙区种子技术服务站,473000,河南南阳
  • 收稿日期:2017-03-17 修回日期:2017-05-02 出版日期:2017-06-15 发布日期:2018-08-26
  • 通讯作者: 宋江春
  • 作者简介:李拴柱,研究实习员,主要从事花生育种和栽培技术研究
  • 基金资助:
    河南省现代农业产业技术体系专项资金(Z2012-05-01)

Advances in Genetics and Breeding of High Oleic Acid Peanut

Li Shuanzhu1,Song Jiangchun1,Wang Jianyu1,Zhang Xiuge1,Qiao Jianli1,Liu Ning2   

  1. 1 Nanyang Academy of Agricultural Sciences,Nanyang 473000,Henan,China
    2 The Seed Technical Service Stations of Wolong District,Nanyang 473000,Henan,China
  • Received:2017-03-17 Revised:2017-05-02 Online:2017-06-15 Published:2018-08-26
  • Contact: Jiangchun Song

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摘要：

随着花生食品产业的发展,高油酸花生越来越受到人们的关注,市场需求逐渐扩大,培育高产、多抗、优质的高油酸花生新品种成为花生育种工作者的重要目标。综述近年来国内外花生高油酸遗传育种的研究进展和成果,并分析讨论当前育种工作中存在的问题及今后的发展方向,以期为我国的高油酸花生育种提供参考。

关键词: 花生, 高油酸, 遗传育种

Abstract:

With the development of peanut industry, people pay more and more attention to high oleic peanut. It becomes one of the major goals for breeders to develop new high-oleic peanut variety with good quality, high and steady yield. In this review we recapitulates the development of high oleic peanut. First, we summarized advances in high-oleic peanut breeding and listed some cultivars released in recent years. Secondly, we discussed the problems and development orientation of high-oleic peanut breeding. And finally we provided some references for the coming breeding work.

Key words: Peanut, High oleic, Genetic breeding

表1

花生高油酸育种中常用的分子标记"

类型
Types		 引物Primers 分子标记Markers 文献
References
CAPS Marker
 af19-F:GATTACTGATTATTGACTT
1056-R:CCAACCCAAACCTTTCAGAG		 Hpy991:ol1型不能被酶切,Ol1型被切成598bp和228bp
 [30]
1344-F:GGAGCTTTAACAACACAA
1345-R:ATATGGGAGCATAAGGGT		 Hpy188I:ol2型被切成521bp和137bp,Ol2型不能被酶切
 [6]
bf19-F:CAGAACCATTAGCTTTG
FAD-R:CTCTGACTATGCATCAG		 Hpy188I: ol2型被切成6段;Ol2型被切成5段
 [6]
AS-PCR
 bf19-F:CAGAACCATTAGCTTTG
FAD-R:CTCTGACTATGCATCAG		 ahFAD2B基因665_666insMITE突变型扩增片断1434bp,野生型扩增片断1220bp [4]
F435-F:ATCCAAGGCTGCATTCTCAC [37]
F435IC-R:CTCCCTGGTGGATTGTTCATGT 参考片断250bp(检测PCR是否成功)
F435WT-R:ACTTCGTCGCGGTCG 扩增193bp条带,Ol1Ol1Ol2Ol2无此条带
F435SUB-R:TGGGACAAACACTTCGTT 扩增203bp条带,ol1ol1型有此条带
F435INS-R:AACACTTCGTCGCGGTCT 扩增195bp条带,ol2ol2突变有此条带
FAD2A-F:GATTACTGATTATTGACTTGCTTTG
FAD2A-G:GTTTTGGGACAAACACTTCTTC(反应1)
FAD2A-A:GTTTTGGGACAAACACTTCTTT(反应2)		 Ol1Ol1和ol1ol1型分别在反应1和反应2有557bp条带, Ol1ol1型在反应1和反应2中都有557bp条带
 [27]
FAD2B-F:CAGAACCATTAGCTTTGTAGTAGTG
FAD2B-C:AACACTTCGTCGCGGTTG(反应3)
FAD2B-A:AACACTTCGTCGCGGTTT(反应4)		 Ol2Ol2和ol2ol2型分别在反应3和反应4有536bp条带,
Ol2ol2型在反应3和反应4中都有536bp条带
FAD2-R:CTCTGACTATGCATCAGAACTTGT 参考片断1.2kb(检测PCR是否成功)
RT-PCR
 FAD2B-F:GCCGCCACCACTCCAAC
FAD2B-R:TGGTTICGGGACAAACACTIC		 探针6FAMACAGGTTCCCTCAGACMGBNFQ检测ol2型;
探针VICACAGGTTCCCTCGACMGBNFQ检测Ol2型		 [38]
FAD2A-F:GCCGCCACCACTCCAACAC
FAD2A-R:GTTATACCATGATACCTTTGATTTTGGTTTTG		 探针6FAMCCTCGACCGCAACG MGBNFQ检测ol1型;
探针VICCCTCGACCGCGACGMGBNFQ检测Ol1型		 [39]

表2

美国育成高油酸花生品种"

品种
Varieties		 类型
Types		 油酸含量或油酸/亚油酸
Oil content or O/L		 PVP号
PVP No.		 品种
Varieties		 类型
Types		 油酸含量或油酸/亚油酸
Oil content or O/L		 PVP号
PVP No.
SunOleic 95R Runner 80 9400148 York Runner 80 200800186
458[4] Runner - 9600242 Florida Fancy[43] Virginia - 200800231
AT225 High Oleic Runner 80 9700010 McCloud[43] Runner - 200800232
SunOleic 97R Runner 80 9700182 Tamnut OL06 Spanish 20.8:1 200800279
GK-7 High Oleic[41] Runner - 9800019 Tamrun OL07[44] Runner - 200800280
AgraTech 1-1[41] Runner - 200000134 Georgia-08V Virginia 83.79 200900238
AgraTech 201[41] Runner - 200000135 Georgia-09B[45] Runner - 201000075
AgraTech VC2[41] Runner - 200000136 107[45] Runner - 201100459
Georgia Hi-O/L Virginia 40:1 200000255 Georgia-11J[46] Virginia - 201200100
Olin Spanish 22.3:1 200200149 Red River Runner Runner 24.5:1 201200244
Tamrun OL01 Runner 13:1 200200150 Tamrun OL11[47] Runner - 201300196
Georgia 02C Runner 32:1 200300050 727[45] Runner - 201300199
Tamrun OL02 Runner 24.1:1 200300170 511[45] Runner - 201400249
Andru II Runner 80 200300179 Georgia-13M[45] Runner - 201400324
ANorden Runner 80 200300205 NuMex 01[48] Valencia 23:1 -
Hull Runner 80 200300207 297[45] Runner - 201500201
GP-1 Runner 80 200300321 Sullivan[49] - - 201500287
Georgia-04S Spanish 34:1 200500121 Wynne[49] - - 201500288
Georgia-05E Virginia 35:1 200600059 OLé Spanish 20:1 201500377
Brantley Virginia 27.8:1 200600071 VENUS Virginia 26:1 201500363
Tamrun OL12[42] Runner - - Webb[50] Runner - 201500394
AT-3085RO[43] Runner - 200700154 157[44] Runner - 201600140
AT-215[43] Runner - 200700261 Lariat Runner 21:1 201600156
Florida 07 Runner 80 200800069

表3

我国育成高油酸花生品种"

品种Varieties 油酸含量(%)
Oleic acid
content		 审(鉴)定 登记证号
Trial ( Appraisal )
registration No.		 品种Varieties 油酸含量(%)
Oleic acid
content		 审(鉴)定 登记证号
Trial ( Appraisal )
registration No.
狮油红4号Shiyouhong 4 75.13 1991年广东审定 花育661 Huayu 661 81.20 皖品鉴登字第1405002
开农H03-3 Kainong H03-3 81.60 皖品鉴登字第0605006 花育663 Huayu 663 80.60 皖品鉴登字第1405003
花育32 Huayu 32 77.80 鲁农审2009040号 花育962 Huayu 962 82.30 皖品鉴登字第1405019
开农61 Kainong 61 76.01 豫审花2012001 花育963 Huayu 963 80.10 皖品鉴登字第1505029
开农176 Kainong 176 76.80 国品鉴花生2013001 花育964 Huayu 964 81.70 皖品鉴登字第1505030
花育662 Huayu 662 82.11 皖品鉴登字第1305001 花育965 Huayu 965 81.50 皖品鉴登字第1505031
花育51 Huayu51 80.31 皖品鉴登字第1305005 花育966 Huayu 966 82.00 皖品鉴登字第1505032
花育52 Huayu 52 81.45 皖品鉴登字第1305006 冀花16 Jihua 16 79.25 国品鉴花生2015006
花育951 Huayu 951 80.47 皖品鉴登字第1305007 开农71 Kainong 71 72.95 豫审花2015002
花育961 Huayu 961 81.20 皖品鉴登字第1305010 豫花37号Yuhua 37 78.00 豫审花2015011
冀花11 Jihua11 80.70 2013年河北鉴定 花育664 Huayu 664 81.90 皖品鉴登字第1505026
冀花13 Jihua 13 79.60 国品鉴花生2014005 花育666 Huayu 666 81.70 皖品鉴登字第1505027
开农1715 Kainong1715 75.60 豫审花2014002 花育667 Huayu 667 80.30 皖品鉴登字第1505028
开农58 Kainong 58 79.40 鄂审油2014006 冀花18号Jihua 18 81.80 冀审花2016015号
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