关键词: 剑麻, 叶片纤维细胞, 透射电镜, 细胞亚显微结构

Abstract:

The purpose of this study was to observe morphological properties of sisal sheet fiber cells and to identify the sampling sites and provide basic experimental references for future studies of sisal sheet fiber cell development. According to the botanical characteristics of leaves and plants, a sampling part was defined, and then used transmission electron microscopy (TEM) observation and analysis of the morphological structural characteristics of the leaf fiber cells of different regions of mature leaves in two growth periods and the proximal end in three maturities degrees, fiber cells in the middle and proximal of old and young sisal were closely related and differ in size. The cells in the center of the leaves were mostly devoid of protoplasts, while protoplasts containing organelles were found at the paraxial end of the leaves. The fibrous cell structure at the proximal end of three maturation stages was further observed. The results showed that various organelles were pushed to the cell edge by vacuoles, and chloroplasts were the most prominent organelles. Among them, the chloroplasts in juvenile and mature leaves were mostly spindle-shaped, the membrane was complete, and a clear lamellar structure and stacking area could be seen. The chloroplasts in the infancy leaves shrinked, the lamellar structure was disordered, the mitochondria swell, vacuoles appear in the cytoplasm, and the phenomenon of increased heterochromatin and separation of the cytoplasmic wall in the nucleus still exists in infancy leaves. From the comprehensive analysis of the image information, it could be concluded that the active site of growth and development of sisal leaf fiber cells is at the paraxial end of the leaf.

Key words: Sisal, Leaf fiber cells, Transmission electron microscopy, Submicroscopic structure