2,4-表油菜素内酯对盐胁迫下大豆种子萌发及幼苗生理生化的影响

doi:10.16035/j.issn.1001-7283.2026.05.007

摘要/Abstract

摘要：

研究2,4-表油菜素内酯（EBL）对盐胁迫下大豆种子萌发及幼苗生理生化的影响，采用0、10^-12、10^-11、10^-10和10^-9 mol/L 5个浓度梯度的EBL浸种处理0、50、100、150和200 mmol/L NaCl胁迫下的大豆种子。结果表明，随着NaCl胁迫程度的不断增加，大豆的根长、株高、发芽率、发芽势、发芽指数、活力指数及含水量均呈逐渐下降的趋势，不同浓度EBL浸种对大豆因盐胁迫产生的抑制作用有不同程度的缓解作用。在100 mmol/L的NaCl胁迫下，EBL浸种的大豆幼苗体内脯氨酸含量、抗氧化酶活性、叶绿素含量、可溶性蛋白及可溶性糖含量显著上升，丙二醛含量显著下降。10^-10 mol/L EBL处理对大豆在盐胁迫下的缓解作用最明显。

关键词: 大豆, 盐胁迫, 2,4-表油菜素内酯（EBL）, 生理生化, 发芽指标

Abstract:

To investigate the effects of 2,4-epibrassinolide (EBL) on seed germination and seedling physiological and biochemical characteristics of soybean under salt stress, soybean seeds that were soaked in five concentrations of EBL (0, 10^-12, 10^-11, 10^-10, and 10^-9 mol/L) were subjected to NaCl stress at 0, 50, 100, 150, and 200 mmol/L. The results showed that with the increasing severity of NaCl stress, the root length, plant height, germination rate, germination potential, germination index, vigor index, and moisture content of soybean all showed a gradual downward trend, while seed soaking with different concentrations of EBL alleviated the inhibitory effects of salt stress on soybean to varying degrees. Under 100 mmol/L NaCl stress, the proline content, antioxidant enzyme activities, chlorophyll content, soluble protein content, and soluble sugar content in EBL-soaked soybean seedlings significantly increased, whereas the malondialdehyde content significantly decreased. Seed soaking with 10^-10 mol/L EBL treatment exhibited the most pronounced alleviating effect on soybean under salt stress.

Key words: Soybean, Salt stress, 2,4-epibrassinolide (EBL), Physiology and biochemistry, Germination index

闫艳华, 杨雅奇, 杨阳. 2,4-表油菜素内酯对盐胁迫下大豆种子萌发及幼苗生理生化的影响[J]. 作物杂志, 2026 (5): 60–66

Yan Yanhua, Yang Yaqi, Yang Yang. Effects of 2,4-Epibrassinolide on Seed Germination and Seedling Physiology and Biochemistry of Soybean under Salt Stress[J]. Crops, 2026(5): 60–66

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