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作物杂志,2022, 第2期: 230–236 doi: 10.16035/j.issn.1001-7283.2022.02.032

• 植物保护 • 上一篇    

绿原酸对烟草疫霉的抑制作用及对烟草黑胫病的防治效果研究

张豫丹1(), 马晓寒1, 李俊领1, 许自成1, 贾玮1,*(), 石秋环2,*()   

  1. 1河南农业大学烟草学院,450002,河南郑州
    2河南省烟草公司洛阳市公司,471003,河南洛阳
  • 收稿日期:2021-10-18 修回日期:2021-12-13 出版日期:2022-04-15 发布日期:2022-04-24
  • 通讯作者: 贾玮,石秋环
  • 作者简介:张豫丹,研究方向为烟草品质生态,E-mail： 519086134@qq.com
  • 基金资助:
    河南省重点研发与推广专项（科技攻关）项目(212102110445)

Inhibitory Effect of Chlorogenic Acid on Phytophthora nicotiana and Its Control Effect on Tobacco Black Shank Disease

Zhang Yudan1(), Ma Xiaohan1, Li Junling1, Xu Zicheng1, Jia Wei1,*(), Shi Qiuhuan2,*()   

  1. 1Tobacco College, Henan Agricultural University, Zhengzhou 450002, Henan, China
    2Luoyang Branch of Henan Tobacco Company, Luoyang 471003, Henan, China
  • Received:2021-10-18 Revised:2021-12-13 Online:2022-04-15 Published:2022-04-24
  • Contact: Jia Wei,Shi Qiuhuan

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摘要：

由烟草疫霉引发的烟草黑胫病（tobacco black shank,TBS）是烟草生产上最具毁灭性的病害之一。绿原酸（CGA）是广泛存在于自然界中的一种酚类物质,具有抗氧化和抑菌等多种生物活性。试验采用离体培养和叶面喷施的方式研究CGA对烟草疫霉的抑制作用及其对TBS的防治效果。结果表明,与对照相比,100μg/mL CGA可抑制烟草疫霉的生长,菌落直径从7.51cm变为5.95cm,二者差异显著;叶片病斑大小从4.60cm显著降低到1.20cm;抗氧化酶（POD、SOD和CAT）及防御酶（PPO）的活性分别显著提高72.6%、54.6%、155.8%和8.2%。此外,喷施CGA的叶片中可溶性蛋白含量从3.0mg/g显著提高到5.3mg/g;可溶性糖含量从2.7mg/g显著下降到1.5mg/g,脯氨酸含量从192.7µg/g显著下降到150.4µg/g。因此,CGA能直接抑制烟草疫霉的生长,有助于提高烟草对TBS的抗性。

关键词: 绿原酸（CGA）, 烟草黑胫病, 烟草疫霉, 渗透调节物质

Abstract:

Tobacco black shank (TBS) caused by Phytophthora nicotianae is one of the most devastating diseases in tobacco production. Chlorogenic acid (CGA) is a natural phenolic compound that exhibits several unprecedented bioactivities including antioxidants and antibacterials. Using in vitro culture and foliar application method, we investigated CGA inhibition on P. nicotianae and CGA control efficiency on TBS, respectively. The results showed that, compared with control, 100g/mL CGA significantly inhibited the growth of P. nicotianae significantly, and the colony diameter changed from 7.51cm to 5.95cm. Spraying the leaves with 100g/mL CGA significantly reduced leaf spot size from 4.60cm to 1.20cm and significantly increased the activities of the antioxidant enzymes POD, SOD and CAT and the defense enzyme PPO by 72.6%, 54.6%, 155.8% and 8.2%, respectively. In addition, the content of soluble protein in leaves sprayed with CGA increased significantly from 3.0mg/g to 5.3mg/g, the content of soluble sugars increased significantly from 2.7mg/g to 1.5mg/g, and the content of proline decreased from 192.7mg/g to 150.4μg/g. Therefore, CGA could directly inhibit the growth of P. nicotiana, improve the resistance to TBS.

Key words: Chlorogenic acid, Tobacco black shank, Phytophthora nicotianae, Osmoregulation substances

表1

不同浓度CGA对烟草疫霉菌径向生长的抑制

处理
Treatment		 菌落直径
Colony diameter (cm)		 抑菌率
Bacteriostatic rate (%)
CK 7.51±0.03a –
CGA1 6.90±0.08b 8.2
CGA2 5.95±0.10c 20.8

图1

不同浓度CGA对烟草疫霉菌径向生长的抑制 同一列中不同小写字母表示在P < 0.05水平上差异显著

图2

CGA2处理对烟草疫霉菌丝形态的影响

图3

CGA2处理对烟草疫霉菌丝细胞的影响

图4

离体烟叶侵染结果

图5

台盼蓝染色结果

图6

烟株侵染结果

图7

喷施CGA对烟叶病斑大小的影响 不同小写字母表示在0.05水平上差异明显,下同

图8

CGA对接种疫霉菌后烟草叶片酶活力的影响

图9

CGA对接种疫霉菌后烟草可溶性糖、可溶性蛋白和脯氨酸含量的影响

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