作物杂志,2023, 第6期: 35–40 doi: 10.16035/j.issn.1001-7283.2023.06.005

• 遗传育种·种质资源·生物技术 • 上一篇    下一篇

黄淮海夏大豆在海南快速加代技术研究

裴春玲1,2(), 谷勇哲2(), 付佳祺1,2, 晁守伟2, 卢倩1(), 邱丽娟2()   

  1. 1哈尔滨师范大学生命科学与技术学院,150025,黑龙江哈尔滨
    2中国农业科学院作物科学研究所/农作物基因资源与遗传改良国家重大科学工程/农业农村部北京大豆生物学重点实验室,100081,北京
  • 收稿日期:2023-01-31 修回日期:2023-11-06 出版日期:2023-12-15 发布日期:2023-12-15
  • 通讯作者: 邱丽娟,主要从事大豆优异基因资源发掘研究,E-mail:qiulijuan@caas.cn;卢倩为共同通信作者,研究方向为环境生物化学,E-mail:luqian@hrbnu.edu.cn
  • 作者简介:裴春玲,研究方向为大豆种质资源,E-mail:clpei1997@163.com
  • 基金资助:
    国家重点研发计划中国和乌拉圭联合实验室合作项目(2018YFE0116900)

Study on the Rapid Generation-Adding Technology of Huang-Huai-Hai Summer Soybean in Hainan

Pei Chunling1,2(), Gu Yongzhe2(), Fu Jiaqi1,2, Chao Shouwei2, Lu Qian1(), Qiu Lijuan2()   

  1. 1College of Life Science and Technology, Harbin Normal University, Harbin 150025, Heilongjiang, China
    2Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/The National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Soybean Biology in Beijing, Ministry of Agriculture and Rural Affairs, Beijing 100081, China
  • Received:2023-01-31 Revised:2023-11-06 Online:2023-12-15 Published:2023-12-15

摘要:

在海南三亚以4个黄淮海地区大豆品种(系)为试验材料,在鼓粒期采集不同厚度的鲜荚,对自然阴干后的种子状态、百粒重及发芽能力进行研究。结果表明,鲜荚厚度为7~8mm的种子发芽率和发芽势均最高,分别达96.7%和85.8%,分别超过正常收获种子的8.6%和10.3%,因此在海南冬季最适宜提前收获的鲜荚厚度为7~8mm。利用提前摘荚技术,从播种至收获需要50d左右,阴干处理15d,一个世代共需要65d。10月底至次年5月初之间在海南种植,可连续繁殖3代,该技术可为大豆的快速育种提供技术支撑。

关键词: 大豆加代, 鲜荚, 果荚厚度, 发芽率, 加速育种

Abstract:

Four soybean varieties (lines) from the Huang-Huai-Hai region were used as experimental materials in Sanya, Hainan province, and fresh pods of different thicknesses were harvested during the seed filling period. The seed state, 100-seed weight and germination capacity after natural drying in the shade were studied. The results showed that, the germination rate and germination potential of fresh pod seeds with thickness of 7-8mm were the highest, reaching 96.7% and 85.8% respectively, which were higher than those of normal harvested seeds by 8.6% and 10.3%, respectively. Therefore, the most suitable thickness of fresh pods for early harvest in Hainan in winter was 7-8mm. Using the technology of early pod picking, it took about 50 days from sowing to harvesting, and 15 days for drying in the shade. A generation had required 65 days in total. Planting in Hainan from the end of October to the beginning of May of the next year, it can reproduce for three generations continuously. This technology provides technical support for rapid soybean breeding.

Key words: Soybean generation-adding, Fresh pod, Pod thickness, Germination rate, Accellerated breeding

图1

不同厚度果荚自然阴干后的种子状态

图2

不同厚度果荚自然阴干后的种子百粒重 不同小写字母表示差异在P < 0.05水平上显著,下同

图3

不同厚度鲜荚自然阴干后的种子发芽率

表1

不同果荚类型间发芽率、发芽势差异分析

果荚厚度
Pod
thickness
发芽率Germination rate 发芽势Germination potential
平均值
Average value
SD 平均值
Average value
SD
5~6mm 87.3b 0.091 73.0b 0.161
6~7mm 93.3ab 0.055 79.7ab 0.081
7~8mm 96.7a 0.020 85.8a 0.076
8~9mm 90.7b 0.066 77.2ab 0.134
9~10mm 90.7ab 0.082 77.2ab 0.235
CK 89.0b 0.021 77.8ab 0.120

图4

不同厚度鲜荚自然阴干后的种子发芽势

图5

4个品种在播种后不同天数果荚厚度的变化

图6

通过提前摘荚加快育种进程的示意图

[1] Hickey L T, Hafeez A N, Robinson H, et al. Breeding crops to feed 10 billion. Nature Biotechnology, 2019, 37(7):744-754.
doi: 10.1038/s41587-019-0152-9 pmid: 31209375
[2] 房裕东, 韩天富. 作物快速育种技术研究进展. 作物杂志, 2019 (2):1-7.
[3] Kothari N, Hague S S, Frelichowski J, et al. Breeding and genetics: Utilization of cotton germplasm in the winter nursery at Tecoman, Mexico for plant breeding training and research. Journal of Cotton Science, 2011, 15(3):271-273.
[4] 吴绍骙. 异地培育玉米自交系在生产上利用可能性的研究. 河南农学院学报, 1961, 1(1):16-40.
[5] Fang Y, Wang L, Sapey E, et al. Speed-breeding system in soybean: integrating off-site generation advancement, fresh seeding, and marker-assisted selection. Frontiers in Plant Science, 2021, 12:717077.
doi: 10.3389/fpls.2021.717077
[6] Watson A, Ghosh S, Williams M J, et al. Speed breeding is a powerful tool to accelerate crop research and breeding. Nature Plants, 2018, 4(1):23-29.
doi: 10.1038/s41477-017-0083-8 pmid: 29292376
[7] Yao Y, Zhang P, Wang H B, et al. How to advance up to seven generations of canola (Brassica napus L.) per annum for the production of pure line populations?. Euphytica, 2016, 209(1):1-7.
doi: 10.1007/s10681-016-1646-x
[8] Stetter M G, Zeitler L, Steinhaus A, et al. Crossing methods and cultivation conditions for rapid production of segregating populations in three grain amaranth species. Frontiers in Plant Science, 2016, 7:816.
doi: 10.3389/fpls.2016.00816 pmid: 27375666
[9] Yan G, Liu H, Wang H, et al. Accelerated generation of selfed pure line plants for gene identification and crop breeding. Frontiers in Plant Science, 2017, 8:1786.
doi: 10.3389/fpls.2017.01786 pmid: 29114254
[10] 赵晓雷, 付从贵, 王奕, 等. 我国大豆快速育种技术的研究现状. 农业科技通讯, 2016(7):4,7.
[11] Roumet P, Morin F. Germination of immature soybean seeds to shorten reproductive cycle duration. Crop Science, 1997, 37(2):521-525.
doi: 10.2135/cropsci1997.0011183X003700020035x
[12] 常从云, 韩天富. 鼓粒期大豆种子的发芽力. 作物杂志, 2000 (5):6-8.
[13] Nagatoshi Y, Fujita Y. Accelerating soybean breeding in a CO2- supplemented growth chamber. Plant and Cell Physiology, 2019, 60(1):77-84.
doi: 10.1093/pcp/pcy189 pmid: 30219921
[14] Samarah N H, Mullen R E, Goggi S, et al. Effect of drying treatment and temperature on soybean seed quality during maturation. Seed Science and Technology, 2009, 37(2):469-473.
doi: 10.15258/sst
[15] Tang G, Song W, Ling X, et al. Sowing seasons and drying methods during post-harvest influence the seed vigour of soybean (Glycine max (L.) Merr.). Acta Physiologiae Plantarum, 2006, 28(3):273-280.
doi: 10.1007/BF02706541
[16] Carandang F M, Shanmugasundaram S, Carpena A L. Rapid generation advancement in soyabeans using immature seeds. Philippine Journal of Crop Science, 2006, 31(2):53-59.
[17] 夏正俊. 大豆光周期反应与生育期基因研究进展. 作物学报, 2013, 39(4):571-579.
[18] 邱丽娟, 常汝镇. 大豆种质资源描述规范和数据标准. 北京: 中国农业出版社, 2005.
[19] 范旭红, 孟凡凡, 郑宇宏, 等. 熟期与品质性状对东北大豆发芽率影响的研究. 吉林农业科学, 2015, 40(2):24-26.
[20] 邵玉彬, 胡兴国, 袁淑明. 大豆成熟度及后熟作用对发芽率的影响. 大豆通报, 1998, 6(3):13-14.
[21] 朱文学, 曹崇文, 戴天红. 干燥过程对大豆发芽质量及发芽率的影响. 华中理工大学学报, 1997, 25(1):73-75.
[22] 唐善德, 成金莲, 黄敏珍. 大豆种子劣变与提高种子发芽率方法研究. 种子, 1993, 67(5):7-12.
[23] 谢皓, 贾秀婷, 陈学珍, 等. 播种深度和种子大小对大豆出苗率和幼苗生长的影响. 农学学报, 2012, 2(6):10-14.
[24] 李建花, 刘宪, 陈现平. 环境对大豆种子发芽率的影响. 安徽农业科学, 2001, 29(1):25-32.
[1] 张笛,苗兴芬,王雨婷. 100份谷子品种资源萌发期耐盐性评价及耐盐品种筛选[J]. 作物杂志, 2019, (6): 43–49
[2] 马梦雪,赵玲玲,唐思,陈贤军,覃瑞. 消毒方法对红花种子发芽率的影响和种子环境细菌研究[J]. 作物杂志, 2018, (6): 162–167
[3] 杨京京,陈江鲁,谢瑞芝,张小伟,丁变红,吴新明,李少昆,李东方. 玉米种子粒重差异对相关发芽指标整齐度的影响[J]. 作物杂志, 2018, (3): 180–184
[4] 鲜孟筑, 杨萍, 胡立勇, 等. 油菜种子萌发成苗期耐低温性评价[J]. 作物杂志, 2015, (5): 116–122
[5] 赵俊香, 刘守伟, 吴凤芝. 盐碱胁迫对4种菊芋材料种子萌发及幼苗生长的影响[J]. 作物杂志, 2015, (1): 133–137
[6] 贾宝艳, 周婵婵, 孙晓雪, 等. 辽宁省水稻种质资源的耐盐性鉴定评价[J]. 作物杂志, 2013, (4): 57–62
[7] 陈红刚, 杜弢, 马小娇, 林丽, 祝建成, 陈垣. 铁棒锤种子品质的初步研究[J]. 作物杂志, 2013, (1): 155–156
[8] 宋柏权, 范有君, 闫志山, 孙秀才, 刘娜, 赵海英, 杨骥. 种子引发技术对甜菜纸筒育苗出苗的影响[J]. 作物杂志, 2012, (6): 135–138
[9] 牛瑞明, 吴文荣, 吴桂丽, 等. 不同药剂浸种对燕麦种子发芽特性的影响[J]. 作物杂志, 2010, (2): 99–101
[10] 冯培煜, 宋瑞连, 王春华. 玉米种子生产过程中发芽率降低的原因及预防措施[J]. 作物杂志, 2008, (4): 105–107
[11] 刘志良, 董宝华, 黄定广. 荞麦芽菜生产技术[J]. 作物杂志, 2006, (1): 52–52
[12] 桑晓璞, 郑祥全, 石国庆, 等. 保护地草莓畸果发生原因及对策[J]. 作物杂志, 2004, (5): 47–47
[13] 张桂香, 宋旭东, 李爱军, 翟世宏. 玉米主要生理病害的发生原因分析与防治对策[J]. 作物杂志, 2004, (2): 35–37
[14] 易福华, 姜立勋, 陈培申, 等. 晚茬麦催芽机播技术[J]. 作物杂志, 2004, (1): 34–35
[15] 俞扬凤, 刘坚宏, 陈国祥, 等. 立克秀拌种对小麦发芽的影响[J]. 作物杂志, 2003, (3): 19–20
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!