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作物杂志,2019, 第1期: 15–21 doi: 10.16035/j.issn.1001-7283.2019.01.003

所属专题： 其他作物

• 遗传育种·种质资源·生物技术 • 上一篇    下一篇

西农979中长穗偃麦草（Thinopyrum ponticum）的遗传成分分析

史娜溶,李静静,吴慧玉,孙道杰,冯毅,王辉,刘新伦,张玲丽   

  1. 西北农林科技大学农学院,712100,陕西杨凌
  • 收稿日期:2018-10-23 修回日期:2018-12-21 出版日期:2019-02-15 发布日期:2019-02-01
  • 通讯作者: 张玲丽
  • 基金资助:
    杨凌示范区科技计划项目(2017NY-19);陕西省技术创新引导专项基金(2018XNCG-G-20);西北农林科技大学科研基金(2017-01)

Genetic Relationship of Xinong 979 and Thinopyrum ponticum Based on Pedigree Analysis and Molecular Markers

Narong Shi,Jingjing Li,Huiyu Wu,Daojie Sun,Yi Feng,Hui Wang,Xinlun Liu,Lingli Zhang   

  1. College of Agronomy, Northwest A & F University, Yangling 712100, Shaanxi, China
  • Received:2018-10-23 Revised:2018-12-21 Online:2019-02-15 Published:2019-02-01
  • Contact: Lingli Zhang

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摘要：

西农979是我国黄淮麦区优质高产、早熟耐寒兼抗赤霉病的小麦新品种。十倍体长穗偃麦草（Thinopyrum ponticum）7E染色体携带有抗赤霉病和抗叶锈病等多种抗性基因。为明确西农979品种的遗传基础,以西农979及其主要供体亲本小偃6号、早优504、陕229、陕213和西农881为材料进行系谱分析,结果表明,西农979及其主要供体亲本陕229、陕213、西农881均为小偃6号的衍生系;小偃6号是十倍体长穗偃麦草的衍生系。在此基础上,利用十倍体长穗偃麦草7E染色体上106个特异分子标记进行分析,发现有6个标记在西农979和小偃6号中扩增出了十倍体长穗偃麦草的特异片段,西农979和小偃6号均携带有十倍体长穗偃麦草7E染色体短臂上分子标记Xwmc653-Xwmc809之间的75.10~77.46cM区段,标记Xcfd31-Xgwm350之间的86.16~87.32cM区段,以及7E染色体长臂标记Xmag1932-Xdauk144之间的147.71~149.51cM区段。结果表明,西农979携带的十倍体长穗偃麦草7E染色体上的遗传物质源自小偃6号,这为进一步研究和利用西农979提供了理论参考。

关键词: 普通小麦, 西农979, 十倍体长穗偃麦草, 遗传分析

Abstract:

Xinong 979 is a famous facultative cultivar with improved quality, high yield potential, and multi-resistance to various diseases, and has been widely extended in the southern Huanghuai Wheat Zone in China. Chromosome 7E of Thinopyrum ponticum carries Fusarium head blight (FHB) resistance gene (Fhb7), leaf rust resistance gene (Lr19) and stem rust resistance gene (Sr25). In this study, the pedigree and 7E chromosome molecular marker of Th. ponticum were used to analyze the genetic relationship among Xinong 979 and its 5 donor parents (Xiaoyan 6, Zaoyou 504, Shaan 229, Shaan 213, and Xinong 881), and Th. Ponticum. Pedigree analysis showed that the Xinong 979, Shaan 229, Shaan 213, and Xinong 881 were all derived from Xiaoyan 6. Xiaoyan 6 was a derivative from Th. ponticum. Six of 106 specific molecular markers of Th. ponticum chromosome 7E showed specific bands of Th. ponticum in Xinong 979 and Xiaoyan 6. Markers of Xwmc653 and Xwmc809 were located on 7ES 75.10-77.46cM; and Xcfd31 and Xgwm350 were located on 7ES 86.16-87.32cM; and Xmag1932 and Xdauk144 were located on 7EL 147.71-149.51cM. The results showed that Xinong 979 was a derivative from Xiaoyan 6. The study provides a reference for further research and utilization of Xinong 979.

Key words: Common wheat, Xinong 979, Thinopyrum ponticum, Genetic analysis

表1

本研究利用的十倍体长穗偃麦草7E染色体上的特异分子标记"

序号
Code		 标记
Marker		 染色体臂
Chromosome arm		 参考来源
Reference		 序号
Code		 标记
Marker		 染色体臂
Chromosome arm		 参考来源
Reference
1 Xbarc70 7ES USDA 54 Xpsp3003 7EL USDA
2 Xbarc76 7EL 55 Xpsp3123 7EL
3 Xbarc154 7ES 56 Xpsr121 7EL Ayala-Navarrete等[25]
4 XBE399084 7EL Ayala-Navarrete等[25] 57 Xpsr129 7EL
5 XBE404744 7EL 58 xsdauk107 7EL Guo等[8]
6 XBE406148 7EL 59 Xsdauk116 7EL
7 XBE445506 7EL 60 Xsdauk118 7EL
8 XBE445653 7EL 61 Xsdauk124 7EL
9 XBE489982 7EL 62 XsdauK13 7EL
10 XBE605194 7EL 63 XsdauK130 7EL
11 XBE637476 7EL 64 Xsdauk138 7EL
12 XBF145935 7EL 65 Xsdauk140 7EL
13 XBF200943 7EL 66 Xsdauk142 7EL
14 XBF483039 7EL 67 XsdauK144 7EL
15 XBG262436 7EL 68 Xsdauk159 7EL
序号
Code		 标记
Marker		 染色体臂
Chromosome arm		 参考来源
Reference		 序号
Code		 标记
Marker		 染色体臂
Chromosome arm		 参考来源
Reference
16 XBG607810 7EL 69 Xsdauk165 7EL
17 XBM137749 7EL 70 XsdauK32 7EL
18 Xcfa2040 7EL USDA 71 Xsdauk336 7EL
19 Xcfa2049 7ES 72 Xsdauk339 7EL
20 Xcfa2106 7EL 73 Xsdauk340 7EL
21 Xcfa2174 7ES 74 xsdauk341 7EL
22 Xcfa2240 7EL 75 Xsdauk342 7EL
23 Xcfd14 7ES 76 XsdauK343 7EL
24 Xcfd21 7ES 77 Xsdauk343 7EL
25 Xcfd22 7ES 78 XsdauK345 7EL
26 Xcfd31 7ES 79 xsdauk345 7EL
27 Xcfd66 7EL 80 Xsdauk348 7EL
28 Xcfe100 7ES Zhang等[21] 81 Xsdauk350 7EL
29 Xcfe19 7EL 82 Xsdauk351 7EL
30 Xcfe202 7EL 83 XsdauK352 7EL
31 Xedm16 7ES Mullan等[20] 84 Xsdauk353 7EL
32 Xedm34 7ES 85 XsdauK355 7EL
33 Xedm105 7EL 86 Xsdauk356 7EL
34 Xedm109 7ES 87 XsdauK4 7EL
35 Xedm154 7ES 88 Xsdauk6 7EL
36 Xedm158 7EL 89 XsdauK60 7EL
37 Xedm335 7EL 90 XsdauK66 7EL
38 Xgwm130 7EL USDA 91 XsdauK71 7EL
39 Xgwm156 7ES 92 Xsdauk75 7EL
40 Xgwm295 7EL 93 Xsdauk8 7EL
41 Xgwm296 7EL 94 Xswes130 7ES Chen等[23]
42 Xgwm333 7EL 95 Xswes157 7EL
43 Xgwm350 7ES 96 Xswes19 7EL
44 Xgwm44 7ES 97 Xswes22 7ES
45 Xgwm471 7EL 98 Xswes354 7EL
46 Xgwm473 7EL 99 Xswes375 7EL
47 Xgwm635 7EL 100 Xswes376 7EL
48 Xksum052 7EL Yu等[22] 101 Xwmc273 7EL USDA
49 Xmag1759 7EL Xue等[24] 102 Xwmc606 7EL
50 Xmag1932 7EL 103 Xwmc653 7ES
51 Xmag2931 7ES 104 Xwmc809 7ES
52 Xmag2934 7ES 105 Xwmc83 7ES
53 Xmag3283 7ES 106 Xwmc702 7ES

图1

小麦品种西农979及其主要供体材料的系谱简图[15,26-28]"

图2

部分标记在西农979及其主要供体亲本中的扩增结果"

表2

在西农979及其系谱材料中扩增出十倍体长穗偃麦草特异条带的标记及扩增片段长度"

标记
Marker		 在染色体7E
上位置(cM)a
Position on 7E		 扩增片段长度Fragment size (bp)
长穗偃麦草
Th. ponticum		 西农979
Xinong 979		 中国春
Chinese spring		 小偃6号
Xiaoyan 6		 早优504
Zaoyou 504		 陕229
Shaan 229		 陕213
Shaan 213		 西农881
Xinong 881
Xwmc653 75.10 180 180 0b 180 180 180 0b 0b
Xwmc809 77.46 161 161 180 161 161 161 161 161
Xcfd31 86.16 236 236 234 236 236 234 136 236
Xgwm350 87.32 198 198 188 198 198 198 198 198
Xmag1932 147.71 2117 2117 2033 2117 2117 2033 2033 2117
XdauK144 149.51 1896, 1869 1896, 1869 1888 1896, 1869 0b 1896, 1869 1896 1896
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