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作物杂志,2020, 第4期: 84–90 doi: 10.16035/j.issn.1001-7283.2020.04.012

• 遗传育种·种质资源·生物技术 • 上一篇    下一篇

小麦转录因子基因TaMYB70的分离和表达分析

徐园园1(), 赵鹏2, 洪权春1, 朱晓琴1, 裴冬丽1()   

  1. 1商丘师范学院生物与食品学院/植物与微生物互作河南省高校重点实验室,476000,河南商丘
    2商丘师范学院人文学院,476000,河南商丘
  • 收稿日期:2019-12-16 修回日期:2020-03-03 出版日期:2020-08-15 发布日期:2020-08-11
  • 通讯作者: 裴冬丽
  • 基金资助:
    河南省高等学校重点科研项目(16A210037);河南省科技攻关项目(182102110389);河南省自然科学基金(182300410058)

Isolation and Expression Analysis of Transcription Factor Gene TaMYB70 in Wheat

Xu Yuanyuan1(), Zhao Peng2, Hong Quanchun1, Zhu Xiaoqin1, Pei Dongli1()   

  1. 1School of Biology and Food Sciences, Shangqiu Normal University/Henan Provincial Key University Laboratory of Plant-Microbe Interactions, Shangqiu 476000, Henan, China
    2School of Humanities, Shangqiu Normal University, Shangqiu 476000, Henan, China
  • Received:2019-12-16 Revised:2020-03-03 Online:2020-08-15 Published:2020-08-11
  • Contact: Pei Dongli

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摘要：

为了探究小麦MYB转录因子基因TaMYB70的功能,采用同源克隆方法分离TaMYB70（MK024291）基因cDNA序列,运用生物信息学手段分析该基因序列特征,采用实时荧光定量反转录PCR（qRT-PCR）检测其在不同逆境胁迫下的表达模式。结果表明,分离得到的TaMYB70部分序列长度为1 272bp,包含一个长度为1 008bp的开放阅读框,编码335个氨基酸残基。TaMYB70蛋白含有2个螺旋-转角-螺旋结构的Myb-type HTH DNA结合结构域。TaMYB70氨基酸序列与粗山羊草、二穗短柄草等植物MYB44同源性较高,与拟南芥R2R3-MYB转录因子第22亚族成员属于同一分支。qRT-PCR分析表明,TaMYB70在脱落酸胁迫下表达量升高,在PEG和NaCl胁迫下表达量下降,该转录因子可能参与小麦逆境胁迫应答。

关键词: 小麦, TaMYB70, 转录因子, R2R3-MYB, 表达分析

Abstract:

To excavate the function of MYB transcription factor TaMYB70 in wheat, TaMYB70 gene was isolated using homology cloning strategy. The expression patterns of TaMYB70 under different stresses were examined by quantitative real-time PCR (qRT-PCR). The results showed that the TaMYB70 partial cDNA was 1 272bp and contained an open reading frame of 1 108bp, encoding 335 amino acids. TaMYB70 protein contained two Myb-type HTH DNA binding domains which were helix-turn-helix motifs. The homologous sequence alignment indicated that the TaMYB70 had higher homology with MYB44 from other plants, such as Aegilops tauschii and Brachypodium distachyum (L.). TaMYB70 belongs to the same branch as the 22nd members of the arabidopsis R2R3-MYB transcription factors. The expression of TaMYB70 was upregulated under ABA treatment, and downregulated under PEG and NaCl stresses, and which may be involved in stress response of wheat.

Key words: Wheat, TaMYB70, Transcription factor, R2R3-MYB, Expression analysis

表1

本研究所用qRT-PCR引物

引物名
Primer name		 引物序列(5′-3′)
Primer sequence (5′-3′)		 产物长度
Length of PCR
product (bp)
ACTIN-F TCTGTCCTTGTATGCCAGCG 195
ACTIN-R GCGGTTGTTGTGAGGGAGTA
MYB-YG-F GGTCCCTCATCAGCAAGTCC 213
MYB-YG-R TCCAGTGGTTCTTGATGGCG

图1

小麦TaMYB70基因扩增结果

图2

小麦TaMYB70蛋白结构域预测

图3

小麦TaMYB70蛋白三级结构模型

图4

小麦TaMYB70蛋白同源序列比对

图5

小麦TaMYB70蛋白结构域基序分析

图6

TaMYB70与拟南芥MYB转录因子系统进化树分析

图7

逆境胁迫下小麦TaMYB70基因的表达特性

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