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作物杂志,2022, 第2期: 230–236 doi: 10.16035/j.issn.1001-7283.2022.02.032

• 植物保护 • 上一篇    

绿原酸对烟草疫霉的抑制作用及对烟草黑胫病的防治效果研究

张豫丹1(), 马晓寒1, 李俊领1, 许自成1, 贾玮1,*(), 石秋环2,*()   

  1. 1河南农业大学烟草学院,450002,河南郑州
    2河南省烟草公司洛阳市公司,471003,河南洛阳
  • 收稿日期:2021-10-18 修回日期:2021-12-13 出版日期:2022-04-15 发布日期:2022-04-24
  • 通讯作者: 贾玮,石秋环
  • 作者简介:张豫丹,研究方向为烟草品质生态,E-mail： 519086134@qq.com
  • 基金资助:
    河南省重点研发与推广专项（科技攻关）项目(212102110445)

Inhibitory Effect of Chlorogenic Acid on Phytophthora nicotiana and Its Control Effect on Tobacco Black Shank Disease

Zhang Yudan1(), Ma Xiaohan1, Li Junling1, Xu Zicheng1, Jia Wei1,*(), Shi Qiuhuan2,*()   

  1. 1Tobacco College, Henan Agricultural University, Zhengzhou 450002, Henan, China
    2Luoyang Branch of Henan Tobacco Company, Luoyang 471003, Henan, China
  • Received:2021-10-18 Revised:2021-12-13 Online:2022-04-15 Published:2022-04-24
  • Contact: Jia Wei,Shi Qiuhuan

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摘要：

由烟草疫霉引发的烟草黑胫病（tobacco black shank,TBS）是烟草生产上最具毁灭性的病害之一。绿原酸（CGA）是广泛存在于自然界中的一种酚类物质,具有抗氧化和抑菌等多种生物活性。试验采用离体培养和叶面喷施的方式研究CGA对烟草疫霉的抑制作用及其对TBS的防治效果。结果表明,与对照相比,100μg/mL CGA可抑制烟草疫霉的生长,菌落直径从7.51cm变为5.95cm,二者差异显著;叶片病斑大小从4.60cm显著降低到1.20cm;抗氧化酶（POD、SOD和CAT）及防御酶（PPO）的活性分别显著提高72.6%、54.6%、155.8%和8.2%。此外,喷施CGA的叶片中可溶性蛋白含量从3.0mg/g显著提高到5.3mg/g;可溶性糖含量从2.7mg/g显著下降到1.5mg/g,脯氨酸含量从192.7µg/g显著下降到150.4µg/g。因此,CGA能直接抑制烟草疫霉的生长,有助于提高烟草对TBS的抗性。

关键词: 绿原酸（CGA）, 烟草黑胫病, 烟草疫霉, 渗透调节物质

Abstract:

Tobacco black shank (TBS) caused by Phytophthora nicotianae is one of the most devastating diseases in tobacco production. Chlorogenic acid (CGA) is a natural phenolic compound that exhibits several unprecedented bioactivities including antioxidants and antibacterials. Using in vitro culture and foliar application method, we investigated CGA inhibition on P. nicotianae and CGA control efficiency on TBS, respectively. The results showed that, compared with control, 100g/mL CGA significantly inhibited the growth of P. nicotianae significantly, and the colony diameter changed from 7.51cm to 5.95cm. Spraying the leaves with 100g/mL CGA significantly reduced leaf spot size from 4.60cm to 1.20cm and significantly increased the activities of the antioxidant enzymes POD, SOD and CAT and the defense enzyme PPO by 72.6%, 54.6%, 155.8% and 8.2%, respectively. In addition, the content of soluble protein in leaves sprayed with CGA increased significantly from 3.0mg/g to 5.3mg/g, the content of soluble sugars increased significantly from 2.7mg/g to 1.5mg/g, and the content of proline decreased from 192.7mg/g to 150.4μg/g. Therefore, CGA could directly inhibit the growth of P. nicotiana, improve the resistance to TBS.

Key words: Chlorogenic acid, Tobacco black shank, Phytophthora nicotianae, Osmoregulation substances

表1

不同浓度CGA对烟草疫霉菌径向生长的抑制

处理
Treatment		 菌落直径
Colony diameter (cm)		 抑菌率
Bacteriostatic rate (%)
CK 7.51±0.03a –
CGA1 6.90±0.08b 8.2
CGA2 5.95±0.10c 20.8

图1

不同浓度CGA对烟草疫霉菌径向生长的抑制 同一列中不同小写字母表示在P < 0.05水平上差异显著

图2

CGA2处理对烟草疫霉菌丝形态的影响

图3

CGA2处理对烟草疫霉菌丝细胞的影响

图4

离体烟叶侵染结果

图5

台盼蓝染色结果

图6

烟株侵染结果

图7

喷施CGA对烟叶病斑大小的影响 不同小写字母表示在0.05水平上差异明显,下同

图8

CGA对接种疫霉菌后烟草叶片酶活力的影响

图9

CGA对接种疫霉菌后烟草可溶性糖、可溶性蛋白和脯氨酸含量的影响

[1] 张成省. 烟草根系分泌物介导的黑胫病抗性机制研究. 北京: 中国农业科学院, 2020.
[2] Zhang X F, Wang Y C, Han X B, et al. A novel bio-fertilizer produced by prickly ash seeds with biochar addition induces soil suppressiveness against black shank disease on tobacco. Applied Sciences, 2021, 11(16):7261.
doi: 10.3390/app11167261
[3] 谭军. 烟草黑胫病游动孢子迁移特征及影响其侵染的主要因素研究. 长沙:湖南农业大学, 2018.
[4] 刘利佳, 徐志强, 何佳, 等. 哈茨木霉菌诱导烟草抗黑胫病代谢差异的研究. 中国农业科技导报, 2021, 23(8):91-105.
[5] 武霖通. 纳米氧化铜对烟草疫霉菌的致毒机制及对黑胫病的防治效果研究. 成都:西南大学, 2019.
[6] 韦良开, 李瑞, 陈凤鸣, 等. 绿原酸的生物学功能及在养殖业中应用研究. 饲料工业, 2019, 40(1):22-26.
[7] Martinez G, Regente M, Jacobi S, et al. Chlorogenic acid is a fungicide active against phytopathogenic fungi. Pesticide Biochemistry and Physiology, 2017, 140:30-35.
doi: 10.1016/j.pestbp.2017.05.012
[8] 沈奇, 金春雁, 张卫明, 等. 蒲公英绿原酸及其包合物对葡萄保鲜作用的研究. 食品科学, 2007, 28(6),332-335.
[9] 何念武, 杨超. 杜仲叶绿原酸对果蔬保鲜作用的试验研究. 江西农业学报, 2018, 30(11):71-75.
[10] Jiao W, Li X, Wang X, et al. Chlorogenic acid induces resistance against Penicillium expansum in peach fruit by activating the salicylic acid signaling pathway. Food Chemistry, 2018, 260:274-282.
doi: 10.1016/j.foodchem.2018.04.010
[11] Xue L J, Guo W, Yuan Y, et al. Constitutively elevated salicylic acid levels alter photosynthesis and oxidative state but not growth in transgenic populus. Plant Cell, 2013, 25(7):2714-2730.
doi: 10.1105/tpc.113.112839
[12] Pinheiro H A, DaMatta F M, Chaves A R M, et al. Drought tolerance in relation to protection against oxidative stress in clones of Coffea canephora subjected to long-term drought. Plant Science, 2004, 167(6):1307-1314.
doi: 10.1016/j.plantsci.2004.06.027
[13] 王学奎. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2006.
[14] Kerschbaum H H, Tasa B A, Schürz M, et al. Trypan blue-adapting a dye used for labelling dead cells to visualize pinocytosis in viable cells. Cellular Physiology and Biochemistry, 2021, 55(S1):171-184.
doi: 10.33594/000000380 pmid: 34156175
[15] 陈娟妮, 鲁梅, 丁伟. 纳米氧化锌对烟草疫霉菌的抑菌作用研究. 植物医生, 2021, 34(2):34-40.
[16] Lehmann S, Serrano M, L'Haridon F, et al. Reactive oxygen species and plant resistance to fungal pathogens. Phytochemistry, 2015, 112:54-62.
doi: 10.1016/j.phytochem.2014.08.027 pmid: 25264341
[17] 开凯. 绿原酸对猕猴桃和樱桃番茄采后病原菌抑制作用及机理研究. 合肥:合肥工业大学, 2020.
[18] Zhou M, Li P, Wu S, et al. Bacillus subtilis CF-3 volatile organic compounds inhibit Monilinia fructicola growth in peach fruit. Frontiers in Microbiology, 2019, 10:1804.
doi: 10.3389/fmicb.2019.01804
[19] 张钰, 唐明. 丛枝菌根真菌对青杨抗溃疡病生物量和抗病酶活性的影响. 菌物学报, 2021, 40(5):1110-1122.
[20] 蒋继志, 孙琳琳, 郭会婧, 等. 几种微生物提取物诱导马铃薯抗晚疫病及机理的初步研究. 植物病理学报, 2010, 40(2):173-179.
[21] 赵芳, 赵正雄, 徐发华, 等. 施氮量对烟株接种黑胫病前、后体内生理物质及黑胫病发生的影响. 植物营养与肥料学报, 2011, 17(3):737-743.
[22] Zouari M, Ben A C, Elloumi N, et al. Impact of proline application on cadmium accumulation,mineral nutrition and enzymatic antioxidant defense system of Olea europaea L. cv Chemlali exposed to cadmium stress. Ecotoxicity Environmental Safety, 2016, 128:195-205.
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