作物杂志,2024, 第1期: 233238 doi: 10.16035/j.issn.1001-7283.2024.01.031
• 植物保护 • 上一篇
王海涛(), 任春梅, 董岩, 李硕, 程兆榜, 季英华()
Wang Haitao(), Ren Chunmei, Dong Yan, Li Shuo, Cheng Zhaobang, Ji Yinghua()
摘要:
为掌握江苏省高粱病毒病的发生情况,从江苏淮安采集高粱疑似病毒病样品20份,进行了分子检测与鉴定。结果发现,使用玉米黄花叶病毒(maize yellow mosaic virus,MaYMV)引物可扩增出与目的片段一致的条带,检出率为80%。利用MaYMV外壳蛋白(CP)基因引物,以阳性样本RNA为模板进行扩增、克隆、酶切验证和测序分析,并构建进化树发现,淮安分离物CP基因与江苏分离物CP基因的进化关系较近。这些结果表明江苏淮安高粱受到了MaYMV侵染,这是该病毒在我国高粱上的首次报道。
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