Crops ›› 2016, Vol. 32 ›› Issue (4): 62-67.doi: 10.16035/j.issn.1001-7283.2016.04.010

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Cloning and Expression Analysis of the Glycosyltransferase Gene LuUGT72E1 in Flax

Yuan Hongmei1,2,Guo Wendong3,Zhao Lijuan4,Yu Ying2,Wu Jianzhong2,Cheng Lili2,Zhao Dongsheng2,Kang Qinghua2,Huang Wengong2,Yao Yubo2,Song Xixia2,Jiang Weidong2,Liu Yan2,Ma Tingfen2,Wu Guangwen2,Guan Fengzhi2   

  1. 1 Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme,Harbin 150086,Heilongjiang,China
    2 Industrial Crops Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,Heilongjiang,China
    3 Nature and Ecology Institute,Heilongjiang Academy of Sciences,Harbin 150040,Heilongjiang,China
    4 Crop Breeding Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,Heilongjiang,China
  • Received:2016-04-19 Revised:2016-06-06 Online:2016-08-15 Published:2018-08-26
  • Contact: Fengzhi Guan

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Abstract:

The total RNA extracted from flax stems was used for RT-PCR, then the full-length open reading frame (ORF) sequence of glycosyltransferase gene (LuUGT72E1) was amplified. The sequence analysis showed that the full-length ORF of LuUGT72E1 consisted of 1 476bp, encoding 491 amino acids. Homology analysis of the deduced amino acid showed 99% identity to UGT72N2 from flax and 58% to 51% identity to UGT72E1 from Populus trichocarpa, Eucalyptus grandis, Manihot esculenta, Gossypium raimondii and Arabidopsis thaliana. The phylogeny analysis showed that this protein clustered with UGT72E1~3 from Arabidopsis and might play key roles in the process of monolignols glucosylation. The gene expression profiling showed that LuUGT72E1 gene could be induced by BR and Brz. This study could provide candidate genes for fibre improvementin flax.

Key words: Flax, Glycosyltransferase, Cloning, Expression analysis

Fig.1

Agarose gel electrophoresis of LuUGT72E1 gene cloning PCR products M:DNA marker(DL2000);1.The product of cDNA cloning"

Fig.2

The nucleotide and deduced amino acid sequences of LuUGT72E1"

Fig.3

Predicted function domain of LuUGT72E1 protein"

Fig.4

Multiple alignment of glycosyltransferase protein sequence"

Fig.5

Phylogenetic relationship of glycosyltransferase gene family beween Flax and Arabidopsis"

Fig.6

Relative expression analysis of lignin biosynthesis related genes induced by BR and Brz"

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