Crops ›› 2016, Vol. 32 ›› Issue (4): 86-92.doi: 10.16035/j.issn.1001-7283.2016.04.014

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Investigation on Faba Bean Germplasm Resistance to Callosobruchus chinensis L.

Zhang Hongyan1,Yang Tao1,Guan Jianping1,Yang Shenghua2,Fang Li1,Du Mengying1,Zong Xuxiao1   

  1. 1 Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China
    2 Linxia Academy of Agricultural Sciences,Linxia 731100,Gansu,China
  • Received:2016-04-07 Revised:2016-07-12 Online:2016-08-15 Published:2018-08-26
  • Contact: Xuxiao Zong

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Abstract:

Callosobruchus chinensis L., a post harvesting storage pest,often causes heavy losses and quality damages during the faba bean storage process, which severely limited the industrial development of faba bean.Breeding and utilization of resistant varieties is the most safe and effective way to prevent and control the damage of Callosobruchus chinensis L. to faba bean. Five hundred accessions of faba bean genetic resources were thoroughly infected by feeding Callosobruchus chinensis L. which continued for two years at room temperature in order to evaluate their resistance. The results showed that the average puncture hole numbers (0.92±0.59) per seed of each accession from spring sowing area was significantly (P<0.05) lower than that (1.05±0.42) from autumn sowing area.The average puncture hole numbers (1.30±0.62) per seed of each genotype of cyan (green, light green, milky) group was significantly (P<0.05) higher than that (0.85±0.43) of dark (purple,black, brown, flesh red) group.Based on their resistance ability of 500 faba bean accessions,five geographic related subgroups could be defined according to cluster analysis of Structure and MEGA softwares.The faba bean accessions,H0048, H0092, H0094, H001455, H001471 and H001787 had been screened out for their high resistance to Callosobruchus chinensis L..

Key words: Faba bean, Genetic resources, Callosobruchus chinensis L, Bruchid-resistant screening, Bruchid-resistant genotypes

Table 1

Evaluation criteria of resistance to Callosobruchus chinensis L."

抗性等级
Level of resistance		 抗性评价
Resistance evaluation		 侵染率(%)
Infection rate
0 免疫（I） 0
1 高抗（HR） 0.1~10.0
3 抗（R） 10.1~30.0
5 中抗（MR） 30.1~50.0
7 中感（MS） 50.1~70.0
9 感（S） 70.1~90.0
11 高感（HS） >90.0

Fig.1

The resistance of faba bean from different area to Callosobruchus chinensis L."

Table 2

Infected faba bean with different colors by Callosobruchus chinensis L."

粒色
Seed color		 材料数（份）
Number of materials		 每粒虫孔数
The average puncture hole numbers per seed
青绿色 178 1.30±0.62a
深色 322 0.85±0.43b

Fig.2

The resistance of faba bean with different colors to Callosobruchus chinensis L."

Table 3

Infected faba bean from different areas by Callosobruchus chinensis L."

生态区
Area		 材料数（份）
Number of materials		 每粒虫孔数
The average puncture hole numbers per seed
秋播 341 1.05±0.42a
春播 159 0.92±0.59b

Fig.3

Population structure of faba bean using Structure"

Table 4

Population structure of faba bean by using Structure"

来源Origin 亚群ⅠSubgroupⅠ 亚群ⅡSubgroupⅡ 亚群ⅢSubgroupⅢ 亚群ⅣSubgroupⅣ 亚群ⅤSubgroupⅤ
安徽Anhui 7 - - - 2
上海Shanghai - - - - 1
福建Fujian - - - 1 -
甘肃Gansu - 13 5 9 8
贵州Guizhou 1 15 6 8 1
河北Hebei 5 - - - 2
湖北Hubei 4 27 6 7 3
湖南Hunan 1 1 - - -
江苏Jiangsu 31 5 3 8 16
内蒙古Inner Mongolia 6 2 16 5 9
宁夏Ningxia - - - - 1
青海Qinghai - 2 8 3 4
山西Shanxi 3 18 7 9 -
陕西Shaanxi - 5 1 2 1
四川Sichuan 9 4 4 1 5
新疆Xinjiang 1 1 4 4 5
云南Yunnan 30 12 23 10 14
浙江Zhejiang 27 5 8 11 22
重庆Chongqing - - - - 2
合计Total 125 110 91 78 96

Fig.4

Dendrogram of provincial group of the faba bean accessions"

[1] 张镇林.蚕豆.上海:上海科学技术出版社, 1960.
[2] 郑卓杰 . 中国食用豆类学.北京: 中国农业出版社, 1997.
[3] 汪小华 . 蚕豆病害叶、蚕豆种子的红外光谱研究. 昆明:云南师范大学, 2014.
[4] 叶茵 . 中国蚕豆学.北京: 中国农业出版社, 2000.
[5] 中国科学院中国植物志编辑委员会. 中国植物志.北京: 科学出版社, 1998.
[6] 龙静宜 . 食用豆类作物.北京: 科学出版社, 1989.
[7] 黄浩东, 周光辉, 胡景兰 .蚕豆粉丝制作工艺.食品科学,1987(7):62-64.
[8] 李庆龙 . 粮食食品加工技术.北京: 中国食品出版社, 1987.
[9] 李智安, 傅翠珍 .中国食用豆类营养品质鉴定与评价.北京: 中国农业科技出版社, 1993.
[10] 孙蕾, 程须珍, 王丽侠 . 绿豆抗豆象研究进展. 植物遗传资源学报, 2007,8(1):113-117.
[11] 杨永茂, 叶向勇, 李玉亮 .瘤背豆象属4种检疫性害虫及其防治.山东农业科学,2004(3):54-56.
[12] 刘长友, 田静, 王素华 , 等. 豇豆属3种主要食用豆类的抗豆象育种研究进展. 中国农业科学, 2010,43(12):2410-2417.
[13] 仲伟文, 杨涛, 段灿星 , 等.豌豆种质资源抗绿豆象鉴定.作物杂志,2014(5):43-47.
[14] Talekar N S . Biology,damage and control of bruchid pests of mungbean.Mungbean:proceedings of the second international symposium. AVRDC, Tainan. 1988: 329-342.
[15] Gujar G T, Yadav T D . Feeding of Callosobruchus maculatuts (Fab.) and Callosobruchus chinensis (Linn.) in green gram. Indian Journal of Entomology, 1978,40(2):108-112.
[16] 张耀文, 赵雪英, 程须珍 , 等.绿豆抗豆象素材的筛选及鉴定//中国绿豆产业发展与科技应用.北京: 中国农业科学技术出版社, 2002: 116-118.
[17] 刘巨元, 张永芳, 刘永平 .内蒙古仓库昆虫.北京: 中国农业出版社, 1997.
[18] Tomooka N, Kashiwaba K, Vaughan D A , et al. The effectiveness of evaluating wild species:searching for sources of resistance to bruchid beetles in the genus Vigna subgenus Ceratotropis. Euphytica, 2000,115(1):27-41.
doi: 10.1023/A:1003906715119
[19] 陈方景, 梁璧元, 程义华 .浙西南山区景宁县绿豆象的发生规律及综合防治技术.农资科技,2004(3):22-23.
[20] Morales H . Pest management in traditional tropical agroecosystems:Lessons for pest prevention research and extension. Integrated Pest Management Reviews, 2002,7(3):145-163.
doi: 10.1023/B:IPMR.0000027502.91079.01
[21] 张文辉, 刘光杰 . 植物抗虫性次生物质的研究概况. 植物学通报, 2003,20(5):522-530.
[22] 周吉红, 吴绍宇, 程须珍 , 等.绿豆抗豆象育种品系抗性鉴定//中国绿豆产业发展与科技应用.北京: 中国农业科学技术出版社, 2002: 119-121.
[23] Tomooka N, Lairungreang C, Nakeeraks P , et al. Development of bruchid-resistant mungbean line using wild mungbean germplasm in thailand. Plant Breeding, 1992,109(1):60-66.
doi: 10.1111/pbr.1992.109.issue-1
[24] Hardie D C, Baker G J, Marshall D R . Field screening of Pisum accessions to evaluate their susceptibility to the pea weevil (Coleoptera:Bruchidae). Euphytica, 1995,84(2):155-161.
doi: 10.1007/BF01677954
[25] 宗绪晓, 包世英, 关建平 , 等.蚕豆种质资源描述规范和数据标准.北京: 中国农业出版社, 2006.
[26] 郑卓杰 . 中国蚕豆种质资源研究及生态区的初步划分//杭州国际蚕豆会议学术讨论会论文, 1989: 24-26.
[27] 刘旭明, 金达生, 程须珍 , 等.绿豆种质资源抗豆象鉴定研究初报.作物品种资源,1998(2):35-37.
[28] 王进忠, 田慧敏, 孙淑玲 , 等. 绿豆象生物学习性及室内药效测定. 北京农学院学报, 2005,20(4):25-44.
[29] 邓永学, 李隆术 . 温度对绿豆象地理种群生长发育的影响. 西南农业大学学报, 1990,12(4):343-345.
[30] 吴春芳 . 鲜食蚕豆新品种通鲜1号选育及其配套栽培技术研究. 南京:南京农业大学, 2005.
[31] 何会超, 吴冬梅, 汪自强 .豆类的硬实与种质资源的保存//2011年中国作物学会学术年会论文摘要集, 2011.
[32] 范六民 . 植物体内的单宁. 生物学通报, 1996,36(3):33.
[33] 何强, 姚开, 石碧 . 植物单宁的营养学特性. 林产化学与工业, 2001
21(1):80-85.
[34] 冷平, 张国军, 吴晓云 , 等. 秋冬季节柿属植物树体内酚类物质含量的变化. 中国农业大学学报, 2001,6(1):63-67.
[35] 覃伟权, 彭正强, 刘济宁 . 植物次生物质研究进展. 热带农业科学, 2002,22(6):60-68.
[36] 黄天芳 . 植物次生物质对于植物生存的重要作用. 生物学杂志, 2003,20(5):60-61.
[37] 姚英娟, 薛东, 杨长举 . 植物源农药在储粮害虫防治中的应用. 粮食储藏, 2004,32(2):6-9.
[38] 徐汉虹, 张志祥, 查友贵 . 中国植物性农药开发前景. 农药, 2003,42(3):1-10.
[39] 杨长举, 杨志慧, 程志强 , 等. 对绿豆象的辐射遗传效应. 植物保护学报, 1993,20(4):331-336.
[40] 杨长举, 杨志慧, 邓望喜 , 等. 几种植物性物质防治绿豆象的初步研究. 中国粮油学报, 1994,9(2):4-9.
[41] 张文辉, 刘光杰 . 植物抗虫性次生物质的研究概况. 植物学通报, 2003,20(5):522-530.
[42] Rizvi S J H, Pandey S K, Mukerji D , et al. 1,3,7-Trimethylxanthine,a new chemosterilant for stored grain pest,Callosobruchus chinensis (L.). Zeitschrift Für Angewandte Entomologie, 1980,90(1-5):378-381.
[43] Ahmed S M, Eapen M . Vapour toxicity and repellency of some essential oils to insect pests. Indian Perfumer, 1986,30(1):273-278.
[44] 陆惠生 . 温水烫杀绿豆象最适温度试验.广西农业科学,2000(2):75-76.
[45] 高美须, 王传耀, 张生芳 , 等. 辐照作为豆类中绿豆象的检疫处理方法. 植物检疫, 2004,18(1):11-14.
[46] 宗绪晓 . 蚕豆的营养特点加工技术和利用途径.中国粮油学报,1993(8):51-54.
[47] 王志斌, 张秀梅, 郭三堆 . 在转基因植物中利用植物凝集素防治害虫的研究. 植物学通报, 2000,17(2):108-113.
[48] 赫卫清, 潘一廷, 唐益雄 . 淀粉酶抑制剂与抗虫的研究进展. 生命的化学, 2002,22(2):172-174.
[49] 严清彪, 刘玉皎 . 不同蚕豆品种的品质分析. 安徽农业科学, 2012,40(31):15153-15154.
[50] 朱南山, 张彬, 李丽立 .单宁的抗营养作用机理及处理措施.中国饲料,2006(17):26-28.
[51] 李洪山, 戴华国, 李慈厚 , 等.利用植物的防御性倡导害虫治理的新思路.大麦科学,2005(3):23-27.
[52] 周嘉熹, 杨雪彦, 宋占邦 .树木单宁可作为对两种星天牛的抗性指标.陕西林业科技,1996(4):15-18.
[53] 朱晓茵, 刘玉萍 .高粱单宁含量与粒色的关系.杂粮作物,1994(2):54-55.
[54] 狄莹, 石碧 .植物单宁化学研究进展.化学通报,1999(3):1-5.
[55] 王强, 邓永学, 王进军 , 等. 绿豆象西昌种群和沈阳种群的杂交研究. 西南大学学报(自然科学版), 2008,30(2):133-137.
[56] 黄建国 . 绿豆象的生物学研究.郑州粮食学院学报,1980(4):22-28.
[57] 王燕, Narayan S T, >陈斌, 等. 绿豆象在不同豆类上的生长发育及产卵选择性研究. 云南农业大学学报, 2010,25(1):34-39.
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