Crops ›› 2017, Vol. 33 ›› Issue (6): 50-54.doi: 10.16035/j.issn.1001-7283.2017.06.009

Previous Articles     Next Articles

EMS Mutant Screening and RAPD Analysis on Industrial Hemp (Cannabis sativa L.)

Jiang Ying,Sun Yufeng,Pan Dongmei,Li Qiuzhi   

  1. Daqing Branch of Heilongjiang Academy of Sciences,Daqing 163319,Heilongjiang,China
  • Received:2017-07-26 Revised:2017-09-13 Online:2017-12-15 Published:2018-08-26

RichHTML

4

PDF (PC)

196

Abstract:

Mutant library is the basis of functional genomics research. In this study, seeds of hemp varieties Huoma No.1 were treated by chemical mutagen Ethyl sulfonate (EMS). Mutation plants were screened in M2 generation according to agronomic and biological traits. 37 mutants were acquired from seedling stage to adult stage. 27 mutants were found in seedling stage and 10 mutants were found in adult stage, correspondingly mutant percentages were 20.93% and 11.24%. Furthermore, mutants were screened by RAPD (Random Amplified Polymorphic DNA), 11 out of 26 random primers could detect DNA polymorphism. Results showed the mutants varied not only on phenotypic changes, but also on the DNA level, which provided a theoretical and practical basis for the research of functional genomics and breeding direction in EMS mutation population of industrial hemp.

Key words: Industrial hemp (Cannabis sativa L.), EMS, Mutants, RAPD

Table 1

The selected primers used in amplification and their sequences (5′-3′)"

引物Primer 序列Sequence 引物Primer 序列Sequence
OPU-05 TTGGCGGCCT OPS-11 AGTCGGGTGG
OPU-12 TCACCAGCCA OPS-17 TGGGGACCAC
OPU-15 ACGGGCCAGT OPV-06 ACGCCCAGGT
OPX-13 ACGGGAGCAA OPV-08 GGACGGCGTT
OPX-17 GACACGGACC OPW-09 GTGACCGAGT
OPY-05 GGCTGCGACA OPW-16 CAGCCTACCA
OPY-11 AGACGATGGG OPZ-06 GTGCCGTTCA
OPY-15 AGTCGCCCTT OPZ-13 GACTAAGCCC
OPT-07 GGCAGGCTGT OPH-18 GAATCGGCCA
OPT-13 AGGACTGCCA OPI-07 CAGCGACAAG
OPP-12 AAGGGCGAGT OPR-08 CCCGTTGCCT
OPP-16 CCAAGCTGCC OPR-13 GGACGACAAG
OPP-17 TGACCCGCCT OPO-12 CAGTGCTGTG

Fig.1

DNA extraction from mutations of hemp DNA marker：1kb plus DNA ladder"

Fig.2

Amplification of genomic DNAs using primer OPP-12 1, No mutagenic Huoma No.1 materials; 2-9, Mutagenic Huoma No.1 materials; the same below"

Fig.3

Amplification of genomic DNAs using primer OPV-08"

Fig.4

Amplification of genomic DNAs using primer OPY-11"

Fig.5

Amplification of genomic DNAs using primer OPU-05"

[1] 徐艳花, 陈锋, 董中东 , 等. EMS诱变的普通小麦豫农201突变体库的构建与初步分析. 麦类作物学报, 2010,30(4):625-629.
doi: 10.7606/j.issn.1009-1041.2010.04.007
[2] 王殿奎, 关凤芝 . 黑龙江省大麻生产现状及发展对策. 中国麻业, 2005,27(2):98-101.
[3] 吴广文 . 黑龙江省大麻发展问题和建议. 中国麻业科学, 2007,29(6):356-357,370.
[4] 祝丽英 . 利用EMS花粉诱变创造玉米新种质及其突变体鉴定方法的研究. 青岛:青岛建筑工程学院, 2000.
[5] 姜颖 . EMS对工业大麻种子萌发的影响. 中国麻业科学, 2016,38(6):258-262.
[6] 张凤启, 黄永娟, 杨甜甜 , 等. EMS诱变甘蓝型油菜M2代群体的表型突变研究. 植物遗传资源学报, 2010,11(6):760-765.
[7] 梁红艳 . EMS处理对枇杷试管苗表型及遗传物质的影响. 广东农业科学, 2013,40(4):119-120.
[8] 马艳芝, 陈桂平 . 11份荆芥种质资源遗传多样性的RAPD分析. 中药材, 2017,40(2):311-314.
doi: 10.13863/j.issn1001-4454.2017.02.013
[9] 孙宁, 孙建设, 李增裕 . 苹果砧木耐盐突变体的筛选鉴定及RAPD分析. 河北农业大学学报, 2004,27(5):37-40.
[10] 刘旭 . 油菜早熟突变体综合性状研究及其RAPD分析. 福建:福建农林大学, 2008.
[11] 陈其军, 韩玉珍, 傅永福 , 等. 大麻性别的RAPD和SCAR分子标记. 植物生理学报, 2001,27(2):173-178.
[12] 李仕金, 辛培尧, 郭鸿彦 , 等. 大麻雄性相关RAPD和SCAR标记的研究. 广东农业科学, 2012,39(24):151-154.
[13] Giuseppe M, Andrea C, Manuela B , et al. Occurrence and frequency of putatively Y chromosome linked DNA markers in Cannabis sativa L. Euphytica, 2002,126:211-218.
doi: 10.1023/A:1016382128401
[14] 苏友波, 朱颖, 林春 , 等. 大麻RAPD分子标记的引物筛选. 中国麻业科学, 2002,24(5):12-16.
[15] 樊芳芳 . 大麻酚类化合物的全合成. 河南:郑州大学, 2013.
[16] 陈其本, 余立惠, 杨明 , 等. 大麻栽培利用及发展对策.成都: 电子科技大学出版社, 1993: 294-308.
[17] 卢银 . 大白菜EMS突变体库的创建与筛选鉴定. 河北:河北农业大学, 2014.
[18] 吴秀红 . EMS诱发大豆M2与M3代农艺性状的遗传变异分析. 大豆科学, 2012,31(5):858-860.
[19] 刁钰婵, 陈志斌, 焦杨 , 等. EMS诱变玉米花粉M2代生物学效应研究. 河南农业科学, 2008,3(12):33-36.
doi: 10.3969/j.issn.1000-1700.2006.06.002
[20] 刘根忠, 王云飞, 孟祥飞 , 等. EMS诱变的‘里格尔87-5’番茄M2群体突变体表型及其抗坏血酸研究. 园艺学报, 2017,44(1):120-130.
[21] 闫炯, 付晶, 刘桂茹 , 等. EMS诱变小麦河农822的SSR及SDS-PAGE鉴定. 河北农业大学学报, 2008,31(1):1-5,11.
doi: 10.3969/j.issn.1000-1573.2008.01.001
[22] 张晓勤, 薛大伟, 周伟辉 , 等. 用甲基磺酸乙酯(EMS)诱变的大麦浙农大3号突变体的筛选和鉴定. 浙江大学学报(农业与生命科学版), 2011,37(2):169-174.
doi: 10.3785/j.issn.1008-9209.2011.02.008
[23] 崔光荣, 张子学, 张从宇 , 等. 蝴蝶兰EMS离体化学诱变及再生植株RAPD检测. 激光生物学报, 2010,19(5):603-610.
[24] 崔光荣, 张子学, 张从宇 , 等. 文心兰EMS离体诱变及再生苗RAPD检测. 热带作物学报, 2011,32(2):261-268.
[1] Jingang Liang,Zhengguang Zhang. Advance on Effects of Genetically ModifiedCrops on Soil Ecosystems [J]. Crops, 2017, 33(4): 1-6.
[2] Lianmei Tai,Yuhu Zuo,Yaling Zhang,Yongling Jin,Yongxia Guo,Xuehui Jin,Guanghui Jin. Analysis of Genetic Diversity of Alternaria solani Isolated from Potato in Heilongjiang Province [J]. Crops, 2017, 33(3): 151-156.
[3] Bowen Guo,Xue Zhao,Xiaoshuang Wei,Yanjing Han,Qiangfei Liang,Bo Song,Shanshan Liu. Development of a Set of 7S and 11S Multi-Subunit-Deficient Mutants with Chinese Soybean Genetic Background [J]. Crops, 2016, 32(2): 43-49.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Guangcai Zhao,Xuhong Chang,Demei Wang,Zhiqiang Tao,Yanjie Wang,Yushuang Yang,Yingjie Zhu. General Situation and Development of Wheat Production[J]. Crops, 2018, 34(4): 1 -7 .
[2] Baoquan Quan,Dongmei Bai,Yuexia Tian,Yunyun Xue. Effects of Different Leaf-Peg Ratio on Photosynthesis and Yield of Peanut[J]. Crops, 2018, 34(4): 102 -105 .
[3] Xuefang Huang,Mingjing Huang,Huatao Liu,Cong Zhao,Juanling Wang. Effects of Annual Precipitation and Population Density on Tiller-Earing and Yield of Zhangzagu 5 under Film Mulching and Hole Sowing[J]. Crops, 2018, 34(4): 106 -113 .
[4] Wenhui Huang, Hui Wang, Desheng Mei. Research Progress on Lodging Resistance of Crops[J]. Crops, 2018, 34(4): 13 -19 .
[5] Yun Zhao,Cailong Xu,Xu Yang,Suzhen Li,Jing Zhou,Jicun Li,Tianfu Han,Cunxiang Wu. Effects of Sowing Methods on Seedling Stand and Production Profit of Summer Soybean under Wheat-Soybean System[J]. Crops, 2018, 34(4): 114 -120 .
[6] Mei Lu,Min Sun,Aixia Ren,Miaomiao Lei,Lingzhu Xue,Zhiqiang Gao. Effects of Spraying Foliar Fertilizers on Dryland Wheat Growth and the Correlation with Yield Formation[J]. Crops, 2018, 34(4): 121 -125 .
[7] Xiaofei Wang,Haijun Xu,Mengqiao Guo,Yu Xiao,Xinyu Cheng,Shuxia Liu,Xiangjun Guan,Yaokun Wu,Weihua Zhao,Guojiang Wei. Effects of Sowing Date, Density and Fertilizer Utilization Rate on the Yield of Oilseed Perilla frutescens in Cold Area[J]. Crops, 2018, 34(4): 126 -130 .
[8] Pengjin Zhu,Xinhua Pang,Chun Liang,Qinliang Tan,Lin Yan,Quanguang Zhou,Kewei Ou. Effects of Cold Stress on Reactive Oxygen Metabolism and Antioxidant Enzyme Activities of Sugarcane Seedlings[J]. Crops, 2018, 34(4): 131 -137 .
[9] Jie Gao,Qingfeng Li,Qiu Peng,Xiaoyan Jiao,Jinsong Wang. Effects of Different Nutrient Combinations on Plant Production and Nitrogen, Phosphorus and Potassium Utilization Characteristics in Waxy Sorghum[J]. Crops, 2018, 34(4): 138 -142 .
[10] Na Shang,Zhongxu Yang,Qiuzhi Li,Huihui Yin,Shihong Wang,Haitao Li,Tong Li,Han Zhang. Response of Cotton with Vegetative Branches to Plant Density in the Western of Shandong Province[J]. Crops, 2018, 34(4): 143 -148 .