Crops ›› 2021, Vol. 37 ›› Issue (5): 35-42.doi: 10.16035/j.issn.1001-7283.2021.05.006

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Preparations and Comparative Analysis of Photosynthetic Protein Complexes in Different Crops

Wang Ruqing1,2(), Hua Wei1, Liu Jun1()   

  1. 1Oil Crops Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, Hubei, China
    2Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2020-11-17 Revised:2021-01-25 Online:2021-10-15 Published:2021-10-14
  • Contact: Liu Jun E-mail:365339576@qq.com;liujunocr@caas.cn

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Abstract:

We performed analysis of the similarities and differences of photosynthetic protein complexes from C3, C4, C3-C4 intermediary plants and polyploid plants by using Blue Native (BN)-PAGE technology. Diploid C3 plants Arabidopsis and tobacco, monocot C3 plant rice, C4 plant maize, white cauliflower, C3-C4 intermediary plant MS, C3 plants rape, soybean and peanut were used as materials. To optimize the BN-PAGE system, we demonstrated that the optimal procedures are as follows. The final solubilization concentration of 1% should be used for the membrane protein non-ionic detergent n-Dodecyl-β-D-Maltopyranoside (DM) and the appropriate treatment time should be 10 minutes in Arabidopsis. This optimized system was then employed to analyze photosynthetic complexes (or thylakoids) in different types of crops. Our datas indicated that the photosynthetic complexes and their key subunits were dramatically distinct in terms of protein abundance and composition. We found that the content of PSII core protein D1 was higher in C4, monocots and polyploid crops compared to C3, dicots and diploid crops, respectively. Oryza sativa had the highest amount of Cyt b6f complex in all the crops. In particular, the core subunit Cyt f of Cyt b6f varied substantially between the different crops. The abundance of PSI core subunit PsaA in Zea mays was remarkably higher relative to the other eight crops, and the contents of C4 plants were higher than that of C3 plants. Monocots contained significantly higher level of ATPase core subunit CF1β than dicots. Taken together, our findings provide reference for further improvement of photosynthetic traits and enhancement of photosynthetic efficiency in crops.

Key words: Photosynthesis, Thylakoid membrane complexes, Blue native polyacrylamide gel electrophoresis (BN-PAGE), Photosynthetic protein, Crops

Fig.1

BN/SDS -PAGE analysis of thylakoid membrane protein complexes in the model plant Arabidopsis a: unstained 1D BN-PAGE, b: stained 1D BN-PAGE, c: thylakoid membrane protein complexes insolated by 2D BN/SDS PAGE"

Fig.2

BN-PAGE analysis of thylakoid membranes in different crops a: unstained 1D BN-PAGE in the nine crops, b: stained 1D BN-PAGE in the nine crops"

Fig.3

BN-PAGE coupled with immunoblotting analyses of thylakoid membranes in different crops"

Fig.4

Immunoblotting analysis of photosynthetic proteins in different crops"

Fig.5

Analysis of thylakoid membrane components in different crops by 2D BN/SDS PAGE"

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