Crops ›› 2020, Vol. 36 ›› Issue (2): 188-193.doi: 10.16035/j.issn.1001-7283.2020.02.029

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Effects of Astragalus membranaceus Root Water Extract on Seed Germination and Seedling Growth of Four Crops

Chen Ye1,2,Nan Jing1,Su Caijuan1,Ma Yinshan1,2(),Wei Ning3,Jing Yuxia3   

  1. 1 College of Agriculture and Ecological Engineering, Hexi University, Zhangye 734000, Gansu, China
    2 Key Laboratory of Characteristic Resources Utilization in Hexi Corridor of Gansu Province, Zhangye 734000, Gansu, China
    3 Yadong Biotechnology Co., Ltd., Zhangye 734000, Gansu, China
  • Received:2019-09-27 Revised:2019-11-29 Online:2020-04-15 Published:2020-04-13
  • Contact: Yinshan Ma E-mail:mys54072@sohu.com

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Abstract:

The allelopathic effects of Astragalus membranaceus root water extracts on seed germination and seedling growth of four crops (wheat, barley, soybean, Radix isatidis) were studied by indoor bioassay. The results showed that Astragalus membranaceus root water extracts had significant allelopathic effects on seed germination and seedling growth of these four crops, among which allelopathic inhibition on roots was greater than that on the aerial parts and allelopathic inhibition on seeds germination stage was greater than that on seedlings growth stage. Allelopathic inhibition of germination potential, the longest root length and root-shoot ratio of different receptors was the strongest. Allelopathic sensitivity of the four crops was wheat > Radix isatidis > barley > soybean. Therefore, when choosing rotation and intercropping with astragalus, soybean and barley are preferred, and followed by isatis.

Key words: Astragalus membranaceus, Root water extract, Crops, Allelopathy

Table 1

Effects of root water extract of Astragalus membranaceus on seed germination of different crops"

作物Crop 水浸液浓度(g/mL)
Water extract concentration		 发芽势(%)
Germination potential		 发芽率(%)
Germination rate		 发芽指数
Germination index		 活力指数
Vigor index
小麦Wheat 0(CK) 83.3±6.4aA 98.3±2.9aA 4.7±0.3aA 5.2±1.1aA
0.005 79.7±6.1abA 88.3±7.6bAB 4.3±0.5abA 3.8±1.1bAB
0.010 70.0±5.0abA 81.7±7.6bBC 3.9±0.7bA 3.5±0.7bAB
0.050 65.1±5.0bA 70.0±5.0cC 3.7±0.1bA 3.2±0.4bB
0.100 0.0±0.0cB 0.0±0.0dD 0.0±0.0cB 0.0±0.0cC
大麦Barley 0(CK) 68.3±2.9aA 81.7±2.9aA 3.8±0.1aA 4.7±0.6aA
0.005 70.3±2.9aA 78.3±2.9abAB 3.9±0.1aA 5.2±0.4aA
0.010 65.0±5.0aA 76.7±5.8abAB 3.8±0.3aA 5.1±0.6aA
0.050 60.7±7.6aA 71.7±2.9bAB 3.8±0.6aA 5.0±0.6aA
0.100 58.0±5.0aA 70.0±5.0bB 3.7±0.6aA 4.3±0.0aA
大豆Soybean 0(CK) 56.7±7.6aA 63.3±5.8bB 3.0±0.4aA 33.0±1.6aA
0.005 38.3±2.9bB 65.0±5.0bB 3.1±0.3aA 33.7±3.4aA
0.010 36.7±2.9bB 73.3±2.9aA 3.2±0.1aA 33.9±3.1aA
0.050 35.0±2.0bB 75.1±5.0aA 3.2±0.3aA 34.7±3.6aA
0.100 33.3±2.9bB 74.1±5.0aA 3.2±0.2aA 34.1±0.5aA
板蓝根 0(CK) 63.3±8.4aA 93.3±2.9aA 3.1±1.8aA 0.6±0.5aA
Radix isatidis 0.005 60.7±8.4aA 88.3±5.8aA 3.9±0.3aA 0.8±0.3aA
0.010 41.7±7.6bB 76.7±7.6bcB 3.3±0.4aA 0.7±0.1aA
0.050 35.0±9.2bB 70.1±5.0cC 2.7±1.6aA 0.6±0.2aA
0.100 13.3±2.9cC 50.1±0.0dD 1.9±0.1aA 0.3±0.1aA

Table 2

Effects of root water extract of Astragalus membranaceus on seedling growth of different crops"

作物
Crop		 水浸液浓度
Water extract
concentration (g/mL)		 茎叶鲜重(g/10株)
Fresh weight of stems and
leaves (g/10 plant)		 根鲜重(g/10株)
Root fresh weight
(g/10 plant)		 苗高(cm)
Seedling
height		 最长根长(cm)
The longest
root length		 根冠比
Root-shoot
ratio
小麦 0(CK) 1.11±0.18aA 0.57±0.21aA 15.27±2.58aA 16.97±3.04aA 0.52±0.17aA
Wheat 0.005 0.90±0.11bA 0.49±0.15aA 14.07±1.42aA 16.23±0.51aA 0.43±0.12aA
0.010 0.89±0.07bA 0.37±0.13aA 13.33±0.99aA 15.97±1.06aA 0.41±0.01aAB
0.050 0.87±0.11bA 0.32±0.24aA 12.90±1.15aA 11.47±0.81bB 0.38±0.31aAB
0.100 0.00±0.00cB 0.00±0.00bB 0.00±0.00bB 0.00±0.00cC 0.00±0.00bB
大麦 0(CK) 1.24±0.19bB 0.64±0.32aA 13.30±1.21cB 16.43±3.48aA 0.50±0.20aA
Barley 0.005 1.35±0.08bAB 0.60±0.14aA 13.96±0.55bcAB 16.30±1.97aA 0.42±0.07abA
0.010 1.36±0.04bAB 0.56±0.14aA 14.87±0.45abcAB 15.60±0.80aA 0.42±0.10abA
0.050 1.65±0.13aA 0.50±0.05aA 15.73±0.90aA 13.40±1.60abAB 0.38±0.07abA
0.100 1.31±0.12bB 0.34±0.10aA 15.40±0.98abAB 10.27±1.14bB 0.26±0.09bA
大豆 0(CK) 8.36±1.22bB 1.58±0.20bA 12.17±1.40abA 11.10±1.47aA 0.18±0.04aA
Soybean 0.005 11.93±0.92aA 2.23±0.64aA 12.57±1.02aA 10.73±1.15aA 0.19±0.05aA
0.010 11.18±0.94aA 1.97±0.24abA 11.53±1.20abA 8.87±1.12abA 0.18±0.04aA
0.050 10.75±0.94aAB 1.50±0.24bA 8.97±0.87bcAB 8.60±0.10abA 0.14±0.03aA
0.100 10.60±0.60aAB 1.38±0.27bA 6.57±3.46cB 7.63±2.82bA 0.13±0.03aA
板蓝根 0(CK) 0.16±0.11aA 0.01±0.01bcB 3.23±0.95abA 5.70±1.80aAB 0.11±0.06aA
Radix 0.005 0.20±0.09aA 0.02±0.01aA 3.93±0.59abA 5.97±1.87aA 0.13±0.04aA
isatidis 0.010 0.21±0.02aA 0.02±0.00abAB 4.23±0.11aA 6.33±0.64aA 0.09±0.02aA
0.050 0.19±0.06aA 0.02±0.00bcAB 3.10±1.01abA 3.70±1.95abAB 0.08±0.02aA
0.100 0.17±0.03aA 0.01±0.00cB 2.83±0.06bA 1.70±1.21bB 0.07±0.01aA

Table 3

Allelopathy indexes of root water extract of Astragalus membranaceus on seed germination and seedling growth of different crops"

作物
Crop		 水浸液浓度
Water extract
concentration (g/mL)		 发芽势
Germination potential		 发芽率
Germination rate		 发芽指数
Germination index		 活力指数
Vigor
index		 茎叶鲜重
Fresh weight of
stem sand leaves		 根鲜重
Root fresh weight		 苗高
Seedling height		 最长根长
Longest root length		 根冠比
Root-shoot ratio
小麦 0(CK) -0.00aA -0.00aA -0.00aA -0.00aA -0.00aA -0.00aA -0.00aA -0.00aA -0.00aA
Wheat 0.005 -0.05abA -0.11bAB -0.09abA -0.39bAB -0.23bA -0.16aA -0.09aA -0.05aA -0.21aA
0.010 -0.19abA -0.08bBC -0.20bA -0.20bAB -0.25bA -0.54aA -0.15aA -0.06aA -0.27aAB
0.050 -0.28bA -0.17cC -0.40bA -0.29bB -0.28bA -0.78aA -0.18aA -0.48bB -0.37aAB
0.100 -1.00cB -1.00dD -1.00cB -1.00cC -1.00cB -1.00bB -1.00bB -1.00cC -1.00bB
大麦 0(CK) -0.00aA -0.00aA -0.00aA -0.00aA -0.00bB -0.00aA -0.00cB -0.00aA -0.00aA
Barley 0.005 -0.03aA -0.04abAB -0.01aA -0.10aA -0.08bAB -0.07aA -0.05bcAB -0.01aA -0.19abA
0.010 -0.05aA -0.07abAB -0.01aA -0.07aA -0.09bAB -0.14aA -0.11abcAB -0.05aA -0.19abA
0.050 -0.12aA -0.14bAB -0.14aA -0.05aA -0.25aA -0.28aA -0.15aA -0.23abAB -0.32abA
0.100 -0.18aA -0.17bB -0.17aA -0.10aA -0.05bB -0.88aA -0.14abAB -0.60bB -0.92bA
大豆 0(CK) -0.00aA -0.00bB -0.00aA -0.00aA -0.00bB -0.00bA -0.00abA -0.00aA -0.00aA
Soybean 0.005 -0.48bB -0.03bB -0.02aA -0.02aA -0.30aA -0.29aA -0.03aA -0.03aA -0.05aA
0.010 -0.55bB -0.13aA -0.06aA -0.03aA -0.25aA -0.20abA -0.06abA -0.25abA -0.00aA
0.050 -0.62bB -0.02aA -0.07aA -0.05aA -0.22aAB -0.05bA -0.36cAB -0.29abA -0.29aA
0.100 -0.79bB -0.01aA -0.06aA -0.03aA -0.21aAB -0.14bA -0.85cB -0.45bA -0.38aA
板蓝根

Radix
isatidis
 0(CK) -0.00aA -0.00aA -0.00aA -0.00aA -0.00aA -0.00bcB -0.00abA -0.00aAB -0.00aA
0.005 -0.04aA -0.06aA -0.21aA -0.17aA -0.20aA -0.50aA -0.18abA -0.05aA -0.15aA
0.010 -0.52bB -0.22cB -0.09aA -0.09aA -0.24aA -0.50abAB -0.24aA -0.10aA -0.22aA
0.050 -0.81bB -0.33cC -0.15aA -0.02aA -0.16aA -0.29bcAB -0.04abA -0.54abAB -0.38aA
0.100 -3.75cC -0.86dD -0.57aA -0.04aA -0.06aA -0.00cB -0.14bA -2.35bB -0.57aA

Table 4

Comparison of sensitivive indexes of different crops to root water extract of Astragalus membranaceus"

作物
Crop		 三级Level 3 二级Level 2 一级Level 1
作物水平
Level of crops		 种子萌发阶段
Seed germination stage		 幼苗生长阶段
Seedling growth stage		 发芽势
Germination
potential		 发芽率
Germination rate		 发芽指数
Germination index		 活力指数
Vigor
index		 茎叶鲜重
Fresh weight of stems and leaves		 根鲜重
Root fresh weight		 苗高
Seedling height		 最长根长
The longest root length		 根冠比
Root-shoot ratio
小麦
Wheat		 -0.39 -0.37 -0.40 -0.30 -0.34 -0.32 -0.50 -0.53 -0.50 -0.32 -0.32 -0.37
大麦
Barley		 -0.09 -0.05 -0.12 -0.06 -0.08 -0.06 0.02 0.09 -0.27 0.09 -0.18 -0.32
大豆
Soybean		 -0.08 -0.10 -0.06 -0.49 0.03 0.04 0.03 0.20 0.06 -0.25 -0.20 -0.12
板蓝根
Radix
isatidis		 -0.18 -0.29 -0.06 -0.82 -0.29 -0.09 0.04 0.14 0.26 0.05 -0.55 -0.20
[1] 王雄飞, 刘春生, 高鹏 等. 三七水提液对几种植物种子萌发和幼苗生长的化感作用. 中国农学通报, 2014,30(4):299-303.
[2] 孔垂华, 胡飞, 王朋 . 植物化感(相生相克)作用. 北京:高等教育出版社, 2016.
[3] 赵培强 . 黄芪连作障碍的研究. 兰州:西北师范大学, 2009.
[4] 温燕梅 . 黄芪的化学成分研究进展. 中成药, 2006,28(6):879-883.
[5] 张子龙, 王文全 . 药用植物连作障碍的形成机理及其防治. 中国农业科技导报, 2009,11(6):19-23.
[6] 赵庆芳, 李海燕, 张博 . 蒙古黄芪根系自毒物质初步分离及效应研究. 西北师范大学学报, 2012,48(1):80-83.
[7] 赵旭, 王文丽, 李娟 , 等. 蒙古黄芪根系分泌物的化感自毒效应研究. 土壤与作物, 2019,8(1):102-109.
[8] 郎多勇, 付雪艳, 荣佳旺 , 等. 蒙古黄芪根围土壤水浸液对自身种子发芽及生理特性的影响. 中药材, 2015,38(1):11-13.
[9] 张博 . 蒙古黄芪的化感作用研究. 兰州:西北师范大学, 2008.
[10] 叶文斌, 樊亮 . 党参和黄芪种植地土壤水浸液对玉米化感作用研究. 种子, 2013,32(4):29-33.
[11] 张子龙, 侯俊玲, 王文全 , 等. 三七水浸液对不同玉米品种的化感作用. 中国中药杂志, 2014,39(4):594-600.
[12] 谷岩, 邱强, 王振民 , 等. 连作大豆根际微生物群落结构及土壤酶活性. 中国农业科学, 2012,45(19):3955-3965.
[13] 刘小龙, 马建江, 管吉钊 , 等. 连作对棉田土壤枯、黄萎病菌数量及细菌群落的影响. 棉花学报, 2015,27(1):62-70.
[14] 王庆玲, 董涛, 张子 . 三七对小麦的化感作用. 生态学杂志, 2015,34(2):431-438.
[15] 张磊, 邱黛玉, 魏鹏 , 等. 大蒜根系分泌物不同极性成分对当归发芽及幼苗的影响. 中国现代中药, 2015,17(8):815-820.
[16] 袁秀梅, 耿赛男, 郑梦圆 , 等. 蚕豆根分泌物对紫色土有效养分及微生物数量的影响. 中国生态农业学报, 2016,24(7):910-917.
[17] 董艳, 董坤, 汤利 , 等. 蚕豆根系分泌物中氨基酸含量与枯萎病的关系. 土壤学报, 2015,52(4):919-925.
[18] 林娟, 殷全玉, 杨丙钊 , 等. 植物化感作用研究进展. 中国农学通报, 2007,23(1):68-72.
[19] 张博, 赵庆芳, 郭鹏辉 , 等. 甘肃省重要中草药的化感作用初探. 安徽农业科学, 2008,36(2):601-604.
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