Crops ›› 2020, Vol. 36 ›› Issue (2): 188-193.doi: 10.16035/j.issn.1001-7283.2020.02.029

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Effects of Astragalus membranaceus Root Water Extract on Seed Germination and Seedling Growth of Four Crops

Chen Ye1,2,Nan Jing1,Su Caijuan1,Ma Yinshan1,2(),Wei Ning3,Jing Yuxia3   

  1. 1 College of Agriculture and Ecological Engineering, Hexi University, Zhangye 734000, Gansu, China
    2 Key Laboratory of Characteristic Resources Utilization in Hexi Corridor of Gansu Province, Zhangye 734000, Gansu, China
    3 Yadong Biotechnology Co., Ltd., Zhangye 734000, Gansu, China
  • Received:2019-09-27 Revised:2019-11-29 Online:2020-04-15 Published:2020-04-13
  • Contact: Yinshan Ma E-mail:mys54072@sohu.com

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Abstract:

The allelopathic effects of Astragalus membranaceus root water extracts on seed germination and seedling growth of four crops (wheat, barley, soybean, Radix isatidis) were studied by indoor bioassay. The results showed that Astragalus membranaceus root water extracts had significant allelopathic effects on seed germination and seedling growth of these four crops, among which allelopathic inhibition on roots was greater than that on the aerial parts and allelopathic inhibition on seeds germination stage was greater than that on seedlings growth stage. Allelopathic inhibition of germination potential, the longest root length and root-shoot ratio of different receptors was the strongest. Allelopathic sensitivity of the four crops was wheat > Radix isatidis > barley > soybean. Therefore, when choosing rotation and intercropping with astragalus, soybean and barley are preferred, and followed by isatis.

Key words: Astragalus membranaceus, Root water extract, Crops, Allelopathy

Table 1

Effects of root water extract of Astragalus membranaceus on seed germination of different crops"

作物Crop 水浸液浓度(g/mL)
Water extract concentration		 发芽势(%)
Germination potential		 发芽率(%)
Germination rate		 发芽指数
Germination index		 活力指数
Vigor index
小麦Wheat 0(CK) 83.3±6.4aA 98.3±2.9aA 4.7±0.3aA 5.2±1.1aA
0.005 79.7±6.1abA 88.3±7.6bAB 4.3±0.5abA 3.8±1.1bAB
0.010 70.0±5.0abA 81.7±7.6bBC 3.9±0.7bA 3.5±0.7bAB
0.050 65.1±5.0bA 70.0±5.0cC 3.7±0.1bA 3.2±0.4bB
0.100 0.0±0.0cB 0.0±0.0dD 0.0±0.0cB 0.0±0.0cC
大麦Barley 0(CK) 68.3±2.9aA 81.7±2.9aA 3.8±0.1aA 4.7±0.6aA
0.005 70.3±2.9aA 78.3±2.9abAB 3.9±0.1aA 5.2±0.4aA
0.010 65.0±5.0aA 76.7±5.8abAB 3.8±0.3aA 5.1±0.6aA
0.050 60.7±7.6aA 71.7±2.9bAB 3.8±0.6aA 5.0±0.6aA
0.100 58.0±5.0aA 70.0±5.0bB 3.7±0.6aA 4.3±0.0aA
大豆Soybean 0(CK) 56.7±7.6aA 63.3±5.8bB 3.0±0.4aA 33.0±1.6aA
0.005 38.3±2.9bB 65.0±5.0bB 3.1±0.3aA 33.7±3.4aA
0.010 36.7±2.9bB 73.3±2.9aA 3.2±0.1aA 33.9±3.1aA
0.050 35.0±2.0bB 75.1±5.0aA 3.2±0.3aA 34.7±3.6aA
0.100 33.3±2.9bB 74.1±5.0aA 3.2±0.2aA 34.1±0.5aA
板蓝根 0(CK) 63.3±8.4aA 93.3±2.9aA 3.1±1.8aA 0.6±0.5aA
Radix isatidis 0.005 60.7±8.4aA 88.3±5.8aA 3.9±0.3aA 0.8±0.3aA
0.010 41.7±7.6bB 76.7±7.6bcB 3.3±0.4aA 0.7±0.1aA
0.050 35.0±9.2bB 70.1±5.0cC 2.7±1.6aA 0.6±0.2aA
0.100 13.3±2.9cC 50.1±0.0dD 1.9±0.1aA 0.3±0.1aA

Table 2

Effects of root water extract of Astragalus membranaceus on seedling growth of different crops"

作物
Crop		 水浸液浓度
Water extract
concentration (g/mL)		 茎叶鲜重(g/10株)
Fresh weight of stems and
leaves (g/10 plant)		 根鲜重(g/10株)
Root fresh weight
(g/10 plant)		 苗高(cm)
Seedling
height		 最长根长(cm)
The longest
root length		 根冠比
Root-shoot
ratio
小麦 0(CK) 1.11±0.18aA 0.57±0.21aA 15.27±2.58aA 16.97±3.04aA 0.52±0.17aA
Wheat 0.005 0.90±0.11bA 0.49±0.15aA 14.07±1.42aA 16.23±0.51aA 0.43±0.12aA
0.010 0.89±0.07bA 0.37±0.13aA 13.33±0.99aA 15.97±1.06aA 0.41±0.01aAB
0.050 0.87±0.11bA 0.32±0.24aA 12.90±1.15aA 11.47±0.81bB 0.38±0.31aAB
0.100 0.00±0.00cB 0.00±0.00bB 0.00±0.00bB 0.00±0.00cC 0.00±0.00bB
大麦 0(CK) 1.24±0.19bB 0.64±0.32aA 13.30±1.21cB 16.43±3.48aA 0.50±0.20aA
Barley 0.005 1.35±0.08bAB 0.60±0.14aA 13.96±0.55bcAB 16.30±1.97aA 0.42±0.07abA
0.010 1.36±0.04bAB 0.56±0.14aA 14.87±0.45abcAB 15.60±0.80aA 0.42±0.10abA
0.050 1.65±0.13aA 0.50±0.05aA 15.73±0.90aA 13.40±1.60abAB 0.38±0.07abA
0.100 1.31±0.12bB 0.34±0.10aA 15.40±0.98abAB 10.27±1.14bB 0.26±0.09bA
大豆 0(CK) 8.36±1.22bB 1.58±0.20bA 12.17±1.40abA 11.10±1.47aA 0.18±0.04aA
Soybean 0.005 11.93±0.92aA 2.23±0.64aA 12.57±1.02aA 10.73±1.15aA 0.19±0.05aA
0.010 11.18±0.94aA 1.97±0.24abA 11.53±1.20abA 8.87±1.12abA 0.18±0.04aA
0.050 10.75±0.94aAB 1.50±0.24bA 8.97±0.87bcAB 8.60±0.10abA 0.14±0.03aA
0.100 10.60±0.60aAB 1.38±0.27bA 6.57±3.46cB 7.63±2.82bA 0.13±0.03aA
板蓝根 0(CK) 0.16±0.11aA 0.01±0.01bcB 3.23±0.95abA 5.70±1.80aAB 0.11±0.06aA
Radix 0.005 0.20±0.09aA 0.02±0.01aA 3.93±0.59abA 5.97±1.87aA 0.13±0.04aA
isatidis 0.010 0.21±0.02aA 0.02±0.00abAB 4.23±0.11aA 6.33±0.64aA 0.09±0.02aA
0.050 0.19±0.06aA 0.02±0.00bcAB 3.10±1.01abA 3.70±1.95abAB 0.08±0.02aA
0.100 0.17±0.03aA 0.01±0.00cB 2.83±0.06bA 1.70±1.21bB 0.07±0.01aA

Table 3

Allelopathy indexes of root water extract of Astragalus membranaceus on seed germination and seedling growth of different crops"

作物
Crop		 水浸液浓度
Water extract
concentration (g/mL)		 发芽势
Germination potential		 发芽率
Germination rate		 发芽指数
Germination index		 活力指数
Vigor
index		 茎叶鲜重
Fresh weight of
stem sand leaves		 根鲜重
Root fresh weight		 苗高
Seedling height		 最长根长
Longest root length		 根冠比
Root-shoot ratio
小麦 0(CK) -0.00aA -0.00aA -0.00aA -0.00aA -0.00aA -0.00aA -0.00aA -0.00aA -0.00aA
Wheat 0.005 -0.05abA -0.11bAB -0.09abA -0.39bAB -0.23bA -0.16aA -0.09aA -0.05aA -0.21aA
0.010 -0.19abA -0.08bBC -0.20bA -0.20bAB -0.25bA -0.54aA -0.15aA -0.06aA -0.27aAB
0.050 -0.28bA -0.17cC -0.40bA -0.29bB -0.28bA -0.78aA -0.18aA -0.48bB -0.37aAB
0.100 -1.00cB -1.00dD -1.00cB -1.00cC -1.00cB -1.00bB -1.00bB -1.00cC -1.00bB
大麦 0(CK) -0.00aA -0.00aA -0.00aA -0.00aA -0.00bB -0.00aA -0.00cB -0.00aA -0.00aA
Barley 0.005 -0.03aA -0.04abAB -0.01aA -0.10aA -0.08bAB -0.07aA -0.05bcAB -0.01aA -0.19abA
0.010 -0.05aA -0.07abAB -0.01aA -0.07aA -0.09bAB -0.14aA -0.11abcAB -0.05aA -0.19abA
0.050 -0.12aA -0.14bAB -0.14aA -0.05aA -0.25aA -0.28aA -0.15aA -0.23abAB -0.32abA
0.100 -0.18aA -0.17bB -0.17aA -0.10aA -0.05bB -0.88aA -0.14abAB -0.60bB -0.92bA
大豆 0(CK) -0.00aA -0.00bB -0.00aA -0.00aA -0.00bB -0.00bA -0.00abA -0.00aA -0.00aA
Soybean 0.005 -0.48bB -0.03bB -0.02aA -0.02aA -0.30aA -0.29aA -0.03aA -0.03aA -0.05aA
0.010 -0.55bB -0.13aA -0.06aA -0.03aA -0.25aA -0.20abA -0.06abA -0.25abA -0.00aA
0.050 -0.62bB -0.02aA -0.07aA -0.05aA -0.22aAB -0.05bA -0.36cAB -0.29abA -0.29aA
0.100 -0.79bB -0.01aA -0.06aA -0.03aA -0.21aAB -0.14bA -0.85cB -0.45bA -0.38aA
板蓝根

Radix
isatidis
 0(CK) -0.00aA -0.00aA -0.00aA -0.00aA -0.00aA -0.00bcB -0.00abA -0.00aAB -0.00aA
0.005 -0.04aA -0.06aA -0.21aA -0.17aA -0.20aA -0.50aA -0.18abA -0.05aA -0.15aA
0.010 -0.52bB -0.22cB -0.09aA -0.09aA -0.24aA -0.50abAB -0.24aA -0.10aA -0.22aA
0.050 -0.81bB -0.33cC -0.15aA -0.02aA -0.16aA -0.29bcAB -0.04abA -0.54abAB -0.38aA
0.100 -3.75cC -0.86dD -0.57aA -0.04aA -0.06aA -0.00cB -0.14bA -2.35bB -0.57aA

Table 4

Comparison of sensitivive indexes of different crops to root water extract of Astragalus membranaceus"

作物
Crop		 三级Level 3 二级Level 2 一级Level 1
作物水平
Level of crops		 种子萌发阶段
Seed germination stage		 幼苗生长阶段
Seedling growth stage		 发芽势
Germination
potential		 发芽率
Germination rate		 发芽指数
Germination index		 活力指数
Vigor
index		 茎叶鲜重
Fresh weight of stems and leaves		 根鲜重
Root fresh weight		 苗高
Seedling height		 最长根长
The longest root length		 根冠比
Root-shoot ratio
小麦
Wheat		 -0.39 -0.37 -0.40 -0.30 -0.34 -0.32 -0.50 -0.53 -0.50 -0.32 -0.32 -0.37
大麦
Barley		 -0.09 -0.05 -0.12 -0.06 -0.08 -0.06 0.02 0.09 -0.27 0.09 -0.18 -0.32
大豆
Soybean		 -0.08 -0.10 -0.06 -0.49 0.03 0.04 0.03 0.20 0.06 -0.25 -0.20 -0.12
板蓝根
Radix
isatidis		 -0.18 -0.29 -0.06 -0.82 -0.29 -0.09 0.04 0.14 0.26 0.05 -0.55 -0.20
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