Crops ›› 2021, Vol. 37 ›› Issue (5): 153-159.doi: 10.16035/j.issn.1001-7283.2021.05.023

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Effects of Sweet Potato Rotation and Intercropping on the Microbial Community of Rhizosphere Soil

Hu Qiguo(), Liu Yajun(), Wang Wenjing, Wang Qi, Wang Honggang, Chu Fengli   

  1. Shangqiu Academy of Agriculture and Forestry Sciences, Shangqiu 476000, Henan, China
  • Received:2020-12-14 Revised:2021-06-16 Online:2021-10-15 Published:2021-10-14
  • Contact: Liu Yajun E-mail:huqiguo1234@126.com;liuyajun0812@163.com

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Abstract:

To reveal the response mechanism of soil microbial community in different planting systems of sweet potato, the effects of rotation and intercropping on microbial community structure and carbon source utilization capacity of sweet potato rhizosphere soil were studied by using phospholipid fatty acid (PLFA) and ecological board (BIOLOG ECO) through field positioning experiments. In this study, three treatments were sweet potato continuous cropping, sweet potato-wheat rotation, and sweet potato-corn intercropping. The main results were as follow: compared with the sweet potato continuous cropping treatment, sweet potato rotation and intercropping changed the PLFA biomass of soil microorganisms; the contents of bacteria increased by 21.82% and 38.77%, respectively (P<0.05); the contents of actinomycetes increased by 6.98% and 12.77%, and the biomass of Gram-positive bacteria increased by 28.60% and 63.44%, respectively; the biomass of Gram-negative bacteria increased by 18.21% and 22.29%, and the fungal contents decreased by 16.60% and 13.03%, respectively. With the extension of culture time, the average well color development (AWCD value) of sweet potato-corn intercropping was significantly higher than the other two treatments. The utilization capacity of carboxylic acid compounds, polymers, carbohydrates, amino acids, and amines of the sweet potato-corn intercropping treatment was significantly increased by 17.28%, 14.67%, 54.17%, 36.62%, and 20.00%, respectively, compared with the sweet potato continuous cropping treatment. The result of the multivariate analysis (RDA) showed that the changes of soil microbial community structure and functional diversity were controlled by many factors, the soil available potassium and total nitrogen were the main driving factors. However, sweet potato-wheat rotation and sweet potato-corn intercropping could optimize the soil microbial community structure and enhance the microbial functional diversity, the effect of sweet potato-corn intercropping treatment was better.

Key words: Sweet potato, Rotation, Intercropping, Microbial community

Fig.1

The marker analysis of main PLFA in soil microbial community under the different planting patterns of sweet potato Different lowercase letters indicate significant difference (P<0.05)"

Table 1

PLFA content of soil microbial community under the different planting patterns of sweet potato"

处理
Treatment		 真菌
Fungal (nmol/g)		 细菌
Bacterial (nmol/g)		 放线菌
Act (nmol/g)		 真菌/细菌
F/B		 革兰氏阳性菌
G+ (nmol/g)		 革兰氏阴性菌
G- (nmol/g)		 G+/G-
S-S 7.83±0.39a 11.09±0.75c 6.59±0.88c 0.71±0.01a 4.65±0.74c 6.37±1.49b 0.79±0.11b
S-W 6.53±0.72c 13.51±1.38b 7.05±1.10b 0.49±0.10b 5.98±0.91b 7.53±2.29a 0.80±0.13ab
S-C 6.81±0.79b 15.39±0.95a 7.42±0.33a 0.44±0.02c 7.60±0.84a 7.79±1.63a 0.98±0.08a

Fig.2

The AWCD value of soil microbial community under the different planting patterns of sweet potato"

Table 2

The carbon source utilization capacity of soil microbial community under the different planting patterns of sweet potato"

处理
Treatment		 羧酸类化合物
Carbox		 多聚化合物
Pol		 碳水化合物
Carbon		 芳香化合物
Phe		 氨基酸化合物
Amino		 胺类化合物
Amine
S-S 0.81±0.05b 0.75±0.04b 0.72±0.03c 0.70±0.05a 0.71±0.02c 0.55±0.04b
S-W 0.76±0.11c 0.71±0.03b 1.00±0.08b 0.67±0.07ab 0.80±0.09b 0.62±0.06a
S-C 0.95±0.05a 0.86±0.05a 1.11±0.06a 0.63±0.06b 0.97±0.12a 0.66±0.11a

Fig.3

Principal component analysis of soil microbial community structure, carbon source utilization capacity with soil environmental factors The AP, AK, AN, TN, TP, OM and pH represent the soil available phosphorus, available potassium, alkali hydrolyzable nitrogen, total nitrogen, total phosphorus, organic matter and pH value respectively; The bacterial, Act, fungal, G+, G- represent the soil bacteria, act, fungi, Gram-positive bacteria, Gram-negative bacteria, respectively; The carboh, pol, carbox, phe, amino, amine represent the carbohydrates, polymers, carboxylic acids, aromatic compounds, amino acids, amines; the SS1-SS4, SW1-SW4, and SC1-SC4 represent four repeats of sweet potato continuous cropping, sweet potato-wheat rotation, and sweet potato-corn intercropping, respectively"

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