Crops ›› 2021, Vol. 37 ›› Issue (6): 171-176.doi: 10.16035/j.issn.1001-7283.2021.06.027

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Effects of GA3 and 6-BA on Agronomic Traits and Chemical Components of Flue Cured Tobacco after Topping

Gu Yuchao1(), Yang Yide2(), Yan Min2, Liu Yong2, Yang Jian2, Xiang Jinyou2, Luo Zhushi2, Li Linqiu2, Jing Yanqiu1(), Yang Yang2()   

  1. 1College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, Henan, China
    2Yibin Branch of Sichuan Tobacco Company, Yibin 644002, Sichuan, China
  • Received:2021-04-02 Revised:2021-05-24 Online:2021-12-15 Published:2021-12-16
  • Contact: Jing Yanqiu,Yang Yang E-mail:guyuchaovip@vip.qq.com;757364325@qq.com;jingyanqiu72t@163.com;298989633@qq.com

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Abstract:

Yunyan 87 was used as the experimental material to study the effects of different concentrations of gibberellin (GA3) and 6-benzylaminopurine (6-BA) plant growth regulators on the growth and quality of flue-cured tobacco by foliar spraying after topping. The results showed that, spraying GA3 and 6-BA on the day of topping could increase the leaf area of flue-cured tobacco and maximum increase was observed in case of 100mg/L GA3 and 40mg/L 6-BA. Spraying GA3 and 6-BA could increase the chlorophyll content in tobacco leaves, and chlorophyll content increased with the increase of GA3 and 6-BA concentrations. GA3 and 6-BA mainly regulated carbon and nitrogen compounds, the combination of GA3 and 6-BA could increase contents of the total sugar, reducing sugar and potassium in tobacco leaves, while the use of 6-BA alone could increase nicotine content. When the concentration of GA3 was 50mg/L and 6-BA was 40mg/L, the contents of total sugar and reducing sugar were the highest. Different concentrations of GA3 and 6-BA mainly affected the degradation products of carotenoid and neophytadiene in tobacco flavor. When the concentration of GA3 was 50mg/L and 6-BA was 40mg/L, the contents of carotenoid degradation products and neophytadiene in tobacco were higher.

Key words: Tobacco, Topping, GA3, 6-BA, Agronomic traits, Chemical composition

Table 1

Foliar concentration setting of GA3 and 6-BA mg/L"

处理 GA3 6-BA 处理 GA3 6-BA
T1 0 0 T6 50 40
T2 0 20 T7 100 0
T3 0 40 T8 100 20
T4 50 0 T9 100 40
T5 50 20

Table 2

Agronomic characteristics of flue-cured tobacco with different concentrations of GA3 and 6-BA"

处理
Treatment		 株高
Plant height (cm)		 最大叶长
Maximum leaf length (cm)		 最大叶宽
Maximum leaf width (cm)		 茎围
Stem girth (cm)		 最大叶面积
Maximum leaf area (cm2)
T1 120.61±2.95bc 74.71±1.04e 23.06±0.84f 10.40±0.95a 1102.68±58.68f
T2 120.10±2.244c 74.70±0.96e 23.36±0.57ef 10.17±0.91a 1098.41±31.53f
T3 120.85±2.75bc 75.26±0.94de 23.35±0.64ef 10.36±0.98a 1106.07±53.25ef
T4 122.67±1.64a 75.70±1.38cd 23.85±0.81de 10.53±0.91a 1139.38±53.11de
T5 122.50±1.54a 76.32±1.04bc 24.08±0.63cd 10.71±0.96a 1162.99±44.51cd
T6 122.14±2.00ab 76.67±0.78ab 24.42±0.77bc 10.34±0.79a 1178.40±62.61bc
T7 122.79±1.77a 75.78±0.95cd 24.21±0.64cd 10.85±0.64a 1159.72±59.22cd
T8 123.47±2.21a 77.04±0.70ab 24.84±0.46b 10.54±0.92a 1218.34±46.91ab
T9 123.37±2.41a 77.17±0.95a 25.48±0.69a 10.63±0.92a 1243.46±45.68a
GA3 21.063** 39.386** 28.314** 1.879 30.435**
6-BA 0.027 11.098** 5.701** 0.372 5.830*
GA3×6-BA 0.610 1.596 2.159 0.518 1.532

Table 3

Flue-cured tobacco plastid pigments content with different concentrations of GA3 and 6-BA mg/g"

处理
Treatment		 叶绿素a
Chlorophyll a		 叶绿素b
Chlorophyll b		 类胡萝卜素
Carotenoid
T1 0.61±0.26d 0.21±0.02a 0.25±0.03a
T2 0.69±0.38c 0.22±0.02ab 0.24±0.04ab
T3 0.74±0.25ab 0.22±0.02ab 0.22±0.02ab
T4 0.69±0.35bc 0.21±0.01ab 0.23±0.04ab
T5 0.71±0.20bc 0.22±0.01ab 0.25±0.03ab
T6 0.73±0.15abc 0.24±0.02ab 0.25±0.02abc
T7 0.71±0.25bc 0.22±0.01ab 0.26±0.03bc
T8 0.74±0.20ab 0.24±0.01b 0.26±0.03bc
T9 0.77±0.12a 0.24±0.01b 0.23±0.02c
GA3 19.690** 3.610* 2.643
6-BA 32.559** 3.683* 2.804
GA3×6-BA 4.047* 0.537 2.696

Table 4

Conventional chemical composition of flue-cured tobacco leaves with different concentrations of GA3 and 6-BA %"

处理Treatment 总糖Total sugar 还原糖Reducing sugar 总氮Total N 烟碱Nicotine 钾K 氯Cl
T1 22.11±0.50e 20.28±0.51e 1.83±0.08ab 2.09±0.05bc 1.80±0.03d 0.25±0.01a
T2 23.30±0.28d 21.80±0.28cd 1.78±0.21b 2.30±0.09ab 1.86±0.19cd 0.27±0.03a
T3 24.08±0.28c 22.58±0.28bc 2.04±0.04a 2.48±0.02a 1.99±0.07bcd 0.19±0.05a
T4 22.00±0.14e 20.50±0.14d 1.68±0.06b 1.93±0.14cd 2.11±0.04ab 0.21±0.11a
T5 25.08±0.50b 23.58±0.58ab 1.73±0.10b 1.98±0.23cd 2.22±0.03a 0.25±0.02a
T6 25.43±0.29ab 23.93±0.46a 2.00±0.11a 2.16±0.30bc 2.25±0.12a 0.23±0.05a
T7 23.89±0.18cd 22.39±0.76b 1.65±0.09b 1.74±0.06d 2.14±0.16ab 0.20±0.07a
T8 25.89±0.25a 24.39±0.43a 1.85±0.16ab 1.88±0.13cd 1.92±0.10bcd 0.19±0.04a
T9 25.64±0.54ab 24.14±0.37a 1.63±0.10b 2.08±0.10bc 2.07±0.21abc 0.25±0.03a
GA3 8.731* 11.923* 4.863* 15.235* 14.372* 0.365
6-BA 15.112* 20.328** 4.845* 9.845* 1.682 0.139
GA3×6-BA 7.898** 6.424** 4.499* 0.278 1.811 1.500

Table 5

Contents of aroma substances in flue-cured tobacco with different concentrations of GA3 and 6-BA µg/g"

处理
Treatment		 美拉德反应产物
The Millard reaction
product		 苯丙氨酸类代谢产物
Phenyl-alanine-class
metabolites		 类胡萝卜素降解产物
Carotenoid degradation
products		 新植二烯
Neophytadiene		 类西柏烷类降解产物
Cyeane degradation
products
T1 21.94±3.67a 13.12±2.77a 83.23±3.03a 831.47±14.91def 22.46±4.50a
T2 21.23±4.54a 12.63±3.90a 81.46±14.18a 852.97±13.11cde 32.07±10.70a
T3 21.54±4.24a 13.58±2.40a 81.61±10.71a 896.22±9.12bcd 21.23±1.96a
T4 20.75±3.97a 13.95±3.57a 83.65±15.35a 781.97±35.88ef 33.15±12.10a
T5 26.16±1.69a 15.82±3.90a 81.60±21.64a 953.75±74.81b 25.47±2.16a
T6 26.03±1.19a 15.62±0.85a 92.59±3.27a 1107.66±99.05a 24.49±5.21a
T7 22.79±2.89a 13.28±1.64a 76.66±6.47a 761.25±18.77f 36.36±4.32a
T8 24.28±2.13a 13.95±3.21a 78.00±6.49a 1053.00±18.36a 21.67±4.42a
T9 23.11±2.13a 16.06±1.54a 80.82±4.46a 919.55±16.28bc 19.63±4.03a
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