3个苦荞品种对盐胁迫的生理响应及耐受性评价

doi:10.16035/j.issn.1001-7283.2022.03.021

Abstract

Abstract:

In order to study the physiological responses of different tartary buckwheat varieties to salt stress and evaluation of salt tolerance, in the pot experiment, seedlings of Pheasant tartary buckwheat, Ningku 2 and Hunan tartary buckwheat 7-2 were treated with 0, 50, 100, 150mmol/L NaCl solutions, morphological index, seedling growth index and other physiological and biochemical indexes were determined to analyze the mechanisms of salt tolerance in tartary buckwheat, the salt tolerance of different tartary buckwheat varieties was evaluated by membership function analysis. Results showed that the leaves of three tartary buckwheat varieties showed symptoms of water loss shrinkage, yellowing and decay under salt stress condition and the damage of high concentration was the most obvious. With the increase of NaCl concentration, the wet weight, dry weight, plant height, stem diameter and root length of three varieties showed a decreasing trend. The contents of chlorophyll and MDA, SOD activity increased first and then decreased with the highest at 100mmol/L, POD activity and relative conductivity showed an increasing trend with the highest at 150mmol/L, and root vitality showed a decreasing trend with the lowest at 150mmol/L. The salt tolerance of Ningku 2 > Hunan tartary buckwheat 7-2 > Pheasant tartary buckwheat was determined through a membership function analysis and detailed examination.

Key words: Tartary buckwheat, Seedling, Salt stress, Morphological index, Growth index, Physiological and biochemical indexes

Shi Xian, Li Hongyou, Lu Bingyue, Zhou Yun, Zhao Jiju, Zhao Mengli, Liang Jing, Meng Hengling. Physiological Responses of Three Tartary Buckwheat Varieties to Salt Stress and Evaluation of Salt Tolerance[J].Crops, 2022, 38(3): 149-154.

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处理 Treatment	野鸡苦荞Pheasant tartary buckwheat		宁苦2号Ningku 2		湖南苦荞7-2 Hunan tartary buckwheat 7-2
处理 Treatment	鲜重Fresh weight	干重Dry weight	鲜重Fresh weight	干重Dry weight	鲜重Fresh weight	干重Dry weight
CK	3.12±0.55a	0.282±0.043a	1.80±0.47a	0.155±0.045a	2.64±0.27a	0.186±0.033a
Y1	2.26±0.45b	0.220±0.054b	1.70±0.34a	0.149±0.041a	1.49±0.31b	0.135±0.039b
Y2	2.20±0.43b	0.193±0.051c	1.52±0.44b	0.125±0.039b	1.40±0.27bc	0.109±0.017bc
Y3	1.48±0.18c	0.124±0.020d	1.24±0.45c	0.099±0.016c	1.22±0.19c	0.101±0.026c

处理 Treatment	宁苦2号 Ningku 2			野鸡苦荞 Pheasant tartary buckwheat			湖南苦荞7-2 Hunan tartary buckwheat 7-2
处理 Treatment	株高 Plant height (cm)	茎粗 Stem diameter (mm)	根长 Root length (cm)	株高 Plant height (cm)	茎粗 Stem diameter (mm)	根长 Root length (cm)	株高 Plant height (cm)	茎粗 Stem diameter (mm)	根长 Root length (cm)
CK	11.38±1.92a	3.43±0.44a	13.08±2.45a	17.95±1.54a	3.51±0.25a	14.44±1.22a	11.30±0.60a	3.00±0.07a	10.34±0.74a
Y1	11.13±1.18a	3.28±0.20b	9.81±1.00b	13.64±1.06b	3.31±0.10b	12.05±0.41b	9.88±0.61b	2.88±0.11b	9.28±0.70b
Y2	10.44±0.59b	3.27±0.21b	9.83±1.34b	12.30±0.75c	3.24±0.08c	11.35±0.53bc	9.15±0.43c	2.83±0.06b	9.09±0.66b
Y3	9.66±0.66c	2.99±0.33c	9.46±1.36b	11.10±0.64d	3.10±0.08d	11.08±0.63c	8.86±0.31c	2.38±0.17c	7.90±0.41c

品种 Variety	隶属函数值Membership function value											综合评价值 Comprehensive evaluation value
品种 Variety	鲜重 Fresh weight	干重 Dry weight	株高 Plant height	茎粗 Stem diameter	根长 Root length	叶绿素含量 Chlorophyll content	MDA 含量 MDA content	POD 活性 POD activity	SOD 活性 SOD activity	根系活力 Root activity	相对电导率 Relative conductivity	综合评价值 Comprehensive evaluation value
野鸡苦荞 Pheasant tartary buckwheat	0.4787	0.5111	0.3864	0.4703	0.3429	0.5323	0.4807	0.4885	0.4104	0.4809	0.4674	0.4591
宁苦2号Ningku 2	0.5804	0.5893	0.5766	0.5776	0.2991	0.5645	0.4771	0.5253	0.5435	0.3581	0.4311	0.5021
湖南苦荞7-2 Hunan tartary buckwheat 7-2	0.3292	0.3735	0.3827	0.6342	0.5128	0.5911	0.4977	0.5665	0.4644	0.5150	0.4342	0.4819

Physiological Responses of Three Tartary Buckwheat Varieties to Salt Stress and Evaluation of Salt Tolerance

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