Crops ›› 2022, Vol. 38 ›› Issue (4): 138-145.doi: 10.16035/j.issn.1001-7283.2022.04.019

Previous Articles     Next Articles

Effective Effects of Acrylamide/Carboxymethyl Cellulose/Biochar Composite Hydrogel on Cadmium Stress in Tobacco Seedlings

Du Fu1,2(), Xia Maolin1(), Liu Xinyuan3, Yu Zhaojin1, Zhang Zhan4(), Liu Yunfei1, Ji Xiaoming1()   

  1. 1College of Tobacco, Henan Agricultural University, Zhengzhou 450002, Henan, China
    2China Tobacco Hubei Industry Co., Ltd., Wuhan 430040, Hubei, China
    3Technology Center, Sanmenxia Branch of Henan Tobacco Company, Sanmenxia 472400, Henan, China
    4Technology Center, China Tobacco Henan Industry Co., Ltd., Zhengzhou 450008, Henan, China
  • Received:2021-07-15 Revised:2021-09-26 Online:2022-08-15 Published:2022-08-22
  • Contact: Zhang Zhan,Ji Xiaoming E-mail:834681855@qq.com;2359533942@qq.com;zhangzhan2059729@126.com;xiaomingji@henau.edu.cn

RichHTML

5

PDF (PC)

211

Abstract:

In order to alleviate the toxic effect of cadmium (Cd) on the growth and development of tobacco seedlings, the removal effects of new acrylamide/carboxymethylcellulose/biochar composite hydrogel (AM/CMC/B) on Cd2+ in aqueous solution was investigated, and the effects of AM/CMC/B on alleviating Cd stress of tobacco seedlings was studied by pot experiment. The results showed that AM/CMC/B could effectively remove Cd2+ from water and improve the phenotype of tobacco seedlings under Cd stress. Under AM/CMC/B treatment, the maximum leaf length, maximum leaf width, maximum leaf area, total root length, total root surface area, average root diameter, root tip number and root volume increased significantly by 38.28%, 30.01%, 79.19%, 83.31%, 83.97%, 54.44%, 88.21% and 261.56%, respectively. The fresh and dry weight of aboveground part of tobacco seedlings increased by 98.39% and 84.22%, the fresh and dry weight of underground part of tobacco seedlings increased by 130.67% and 231.82%, respectively. The photosynthetic rate, SPAD value and stomatal conductance of tobacco seedlings significantly increased by 62.03%, 29.61% and 84.42%, the content of malondialdehyde decreased by 36.61%. The antioxidant activity and metabolite content of tobacco seedlings increased. AM/CMC/B could significantly improve the tolerance of tobacco seedlings for Cd stress.

Key words: Carboxymethyl cellulose, Biochar, Hydrogel, Cadmium stress, Tobacco seedlings

Fig.1

FT-IR of AM/CMC/B(a), AM/CMC(b) and B(c)"

Fig.2

Equilibrium adsorption of Cd2+ by different hydrogels under different pH values"

Fig.3

Phenotype of tobacco seedlings under Cd stress for 15 days"

Fig.4

Growth indexes of tobacco seedlings under Cd stress for 15 days Different lowercase letters indicate significant difference at 0.05 level, the same below"

Fig.5

Biomass and leaf RWC of tobacco seedlings under Cd stress for 15 days"

Fig.6

Photosynthetic characteristics and SPAD values of tobacco seedlings under Cd stress for 15 days"

Fig.7

Contents of MDA and Pro, activities of CAT and POD in tobacco seedlings under Cd stress for 15 days"

Table 1

Contents of metabolites in tobacco seedlings under different treatments"

代谢物类别
Metabolite category		 代谢物名称
Metabolite name		 保留时间
Retention time (min)		 含量Content (mg/g)
CK H BH
有机酸Organic acid 苹果酸 5.26 0.255 0.349 0.359
对苯二甲酸 6.91 0.151 0.206 1.407
二十碳五烯酸 8.58 0.000 3.115 0.226
棕榈酸 8.79 1.353 1.871 2.586
亚油酸 10.80 0.239 0.380 0.792
亚麻酸 10.91 1.288 2.221 5.113
硬脂酸 11.26 0.547 0.729 0.993
有机酸总量 3.833 8.872 11.476
萜类Terpenoids 植醇 7.51 0.438 0.610 1.005
α-西柏三烯二醇 11.37 1.838 3.696 4.470
β-西柏三烯二醇 12.28 0.851 1.032 1.274
菜油甾醇 23.86 0.433 0.860 1.574
豆甾醇 24.07 1.563 2.192 3.504
β-谷甾醇 24.47 0.578 1.147 1.763
β-香树脂醇 24.59 0.297 0.540 0.904
鹅去氧胆酸 24.96 0.000 0.000 0.196
萜类总量 5.998 10.076 14.691
烷烃类Alkanes 烷烃Ⅰ 21.34 0.000 0.136 0.258
烷烃Ⅱ 21.60 0.000 0.133 0.197
烷烃Ⅲ 22.07 0.203 0.358 0.729
烷烃Ⅳ 22.22 0.102 0.155 0.301
烷烃Ⅴ 22.59 0.375 0.688 1.314
烷烃Ⅵ 22.79 0.689 0.902 1.538
烷烃Ⅶ 22.99 0.210 0.440 0.519
烷烃Ⅷ 23.13 0.121 0.329 0.461
烷烃Ⅸ 23.72 0.258 0.597 1.163
烷烃Ⅹ 23.96 0.267 0.813 1.240
烷烃总量 2.225 4.550 7.719
其他代谢物Other metabolites 丁基化羟基甲苯 6.51 0.134 0.203 0.258
新植二烯 7.23 3.753 3.423 3.214
十八醇 10.00 0.000 0.000 0.128
4,4'-亚甲基双（2-叔丁基-6-甲基苯酚） 17.82 3.076 3.610 4.691
单硬脂酸甘油酯 20.90 0.354 0.725 1.049
其他代谢物总量 7.317 7.961 9.341
代谢物总量Total metabolites 19.373 31.458 43.227
[1] Jin Y H, Clark A B, Slebos R J C, et al. Cadmium is a mutagen that acts by inhibiting mismatch repair. Nature Genetics, 2003, 34(3):326-329.
doi: 10.1038/ng1172
[2] Mohan D, Kumar H, Sarswat A, et al. Cadmium and lead remediation using magnetic oak wood and oak bark fast pyrolysis bio-chars. Chemical Engineering Journal, 2014, 236(2):513-528.
doi: 10.1016/j.cej.2013.09.057
[3] Rai V, Khatoon S, Bisht S S, et al. Effect of cadmium on growth,ultramorphology of leaf and secondary metabolites of Phyllanthus amarus Schum and Thonn. Chemosphere, 2005, 61(11):1644-1650.
doi: 10.1016/j.chemosphere.2005.04.052
[4] Zhang Y, Xu S, Yang S, et al. Salicylic acid alleviates cadmium-induced inhibition of growth and photosynthesis through upregulating antioxidant defense system in two melon cultivars (Cucumis melo L.). Protoplasma, 2015, 252(3):911-924.
doi: 10.1007/s00709-014-0732-y
[5] Rizwan M, Ali S, Rehman M Z U, et al. A critical review on the effects of zinc at toxic levels of cadmium in plants. Environmental Science and Pollution Research, 2019, 26(7):6279-6289.
doi: 10.1007/s11356-019-04174-6
[6] Li J R, Xu Y M, Lin D S, et al. In situ immobilization remediation of heavy metals in contaminated soils:A review. Ecology and Environmental Sciences, 2014, 4:636-646.
[7] Sarmah D, Karak N. Double network hydrophobic starch based amphoteric hydrogel as an effective adsorbent for both cationic and anionic dyes. Carbohydrate Polymers, 2020, 242(15):116320.
doi: 10.1016/j.carbpol.2020.116320
[8] Qu P, Li Y C, Huang H Y, et al. Urea formaldehyde modified alginate beads with improved stability and enhanced removal of Pb2+,Cd2+,and Cu2+. Journal of Hazardous Materials, 2020, 396(5):122664.
doi: 10.1016/j.jhazmat.2020.122664
[9] Hou X, Li Y, Pan Y, et al. Controlled release of agrochemicals and heavy metal ion capture dual-functional redox-responsive hydrogel for soil remediation. Chemical Communications, 2018, 54(97):13714-13717.
doi: 10.1039/C8CC07872F
[10] Liu H, Xu F, Xie Y, et al. Effect of modified coconut shell biochar on availability of heavy metals and biochemical characteristics of soil in multiple heavy metals contaminated soil. Science of the Total Environment, 2018, 645(15):702-709.
doi: 10.1016/j.scitotenv.2018.07.115
[11] Bandara T, Xu J, Potter I D, et al. Mechanisms for the removal of Cd (II) and Cu (II) from aqueous solution and mine water by biochars derived from agricultural wastes. Chemosphere, 2020, 254(12):126745.
doi: 10.1016/j.chemosphere.2020.126745
[12] Wang H T, Ding J, Chi Q Q, et al. The effect of biochar on soil-plant-earthworm-bacteria system in metal (loid) contaminated soil. Environmental Pollution, 2020, 263:114610.
doi: 10.1016/j.envpol.2020.114610
[13] Hecht E M, Landy D C, Ahn S, et al. Hypothesis:cadmium explains,in part,why smoking increases the risk of cardiovascular disease. Journal of Cardiovascular Pharmacology and Therapeutics, 2013, 18(6):550-554.
doi: 10.1177/1074248413494815 pmid: 24038014
[14] Luo Y, Wei Y, Sun S, et al. Selenium modulates the level of auxin to alleviate the toxicity of cadmium in tobacco. International Journal of Molecular Sciences, 2019, 20(15):3772.
doi: 10.3390/ijms20153772
[15] Zhang H, Xu Z, Huo Y, et al. Overexpression of Trx CDSP32 gene promotes chlorophyll synthesis and photosynthetic electron transfer and alleviates cadmium-induced photoinhibition of PSII and PSI in tobacco leaves. Journal of Hazardous Materials, 2020, 398(5):122899.
doi: 10.1016/j.jhazmat.2020.122899
[16] More P, Agarwal P, Joshi P S, et al. The JcWRKY tobacco transgenics showed improved photosynthetic efficiency and wax accumulation during salinity. Scientific Reports, 2019, 9(1):1-14.
doi: 10.1038/s41598-018-37186-2
[17] Duan M, Feng H L, Wang L Y, et al. Overexpression of thylakoidal ascorbate peroxidase shows enhanced resistance to chilling stress in tomato. Journal of Plant Physiology, 2012, 169(9):867-877.
doi: 10.1016/j.jplph.2012.02.012
[18] Roy S K, Cho S W, Kwon S J, et al. Morpho-physiological and proteome level responses to cadmium stress in sorghum. PLoS ONE, 2016, 11(2):e0150431.
doi: 10.1371/journal.pone.0150431
[19] Han R M, Lefevre I, Ruan C J, et al. NaCl differently interferes with Cd and Zn toxicities in the wetland halophyte species Kosteletzkya virginica (L.) Presl. Plant Growth Regulation, 2012, 68(1):97-109.
doi: 10.1007/s10725-012-9697-z
[20] Shen X, Huang D Y, Ren X F, et al. Phytoavailability of Cd and Pb in crop straw biochar-amended soil is related to the heavy metal content of both biochar and soil. Journal of Environmental Management, 2016, 168(1):245-251.
doi: 10.1016/j.jenvman.2015.12.019
[21] Chen Q, Zhang X, Liu Y, et al. Hemin-mediated alleviation of zinc,lead and chromium toxicity is associated with elevated photosynthesis,antioxidative capacity; suppressed metal uptake and oxidative stress in rice seedlings. Plant Growth Regulation, 2017, 81(2):253-264.
doi: 10.1007/s10725-016-0202-y
[22] Azhar M, Rehman M Z U, Ali S, et al. Comparative effectiveness of different biochars and conventional organic materials on growth,photosynthesis and cadmium accumulation in cereals. Chemosphere, 2019, 227(7):72-81.
doi: 10.1016/j.chemosphere.2019.04.041
[23] Choppala G, Saifullah, Bolan N, et al. Cellular mechanisms in higher plants governing tolerance to cadmium toxicity. Critical Reviews in Plant Sciences, 2014, 33(5):374-391.
doi: 10.1080/07352689.2014.903747
[24] Dawood M, Cao F, Jahangir M M, et al. Alleviation of aluminum toxicity by hydrogen sulfide is related to elevated ATPase,and suppressed aluminum uptake and oxidative stress in barley. Journal of Hazardous Materials, 2012, 209-210(1):121-128.
doi: 10.1016/j.jhazmat.2011.12.076
[25] Zouari M, Ahmed C B, Zorrig W, et al. Exogenous proline mediates alleviation of cadmium stress by promoting photosynthetic activity,water status and antioxidative enzymes activities of young date palm (Phoenix dactylifera L.). Ecotoxicology and Environmental Safety, 2016, 128:100-108.
doi: 10.1016/j.ecoenv.2016.02.015 pmid: 26901506
[26] Pallavi S, Bhushan J A, Shanker D R, et al. Reactive oxygen species,oxidative damage,and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany, 2012(2012):1-26.
[27] Xiao J, Sun J G, Pang B, et al. Isolation and screening of stress-resistant endophytic fungus strains from wild and cultivated soybeans in cold region of China. Applied Microbiology and Biotechnology, 2021, 105(2):755-768.
doi: 10.1007/s00253-020-11048-2
[1] Guo Shuya, Shang Shang, Tang Qining, Zhang Yan, Lu Guangyuan. Effects of Different Rotation Tillage Methods and Biochar on Soil Enzyme Activity, Soil Nutrients and Yield of Wheat and Maize [J]. Crops, 2022, 38(3): 211-217.
[2] Gao Jia, Wang Jiao, Wang Song, Liu Hongjian, Kang Jia, Shen Hong, Wang Haili, Ren Shaoyong. Effects of Biochar-Based Fertilizer on Soil Urease Activity and Yield of Potato [J]. Crops, 2021, 37(6): 134-138.
[3] Wang Kun, Wei Yuewei, Ji Xiaoming, Yun Fei, Zou Kai, Long Zhun. Effects of Combined Application of Biochar-Based Fertilizer and Trichoderma harzianum on the Qualities of Flue-Cured Tobacco and Tobacco-Growing Soil [J]. Crops, 2021, 37(3): 106-113.
[4] Liu Meiju,Li Jiangzhou,Ji Sigui,Fan Miaomiao,Gu Xinghui,Zhang Limeng,Zhang Jinwei,Qu Xing,Zhou Wenbing,Lin Shan. Evaluation of Effect of Biochar on Tobacco Yield and Nitrogen Use Efficiency in Mountain Slope Areas [J]. Crops, 2020, 36(1): 89-97.
[5] Cui Yuefeng,Sun Guocai,Lu Tiegang,Wang Guiyan,Wang Jian,Huang Wenjia,Luan Helin. Effects of Different Straw Return Modes on Nitrogen Absorption and Utilization of Super Rice in Northern China [J]. Crops, 2019, 35(4): 164-169.
[6] Jing Yan,Wenxiu Ji,Xianji Shi,Shimiao Zhu,Hulin Li. Effects of Cadmium Stress on Seed Germination and Seedling Growth of Tobacco (Nicotiana tabacum) [J]. Crops, 2019, 35(2): 142-149.
[7] Siyu Gong,Sirong Zhong,Shichuan Zhang,Yaping Nie,Xihuan Liang,Shuaiqiang Yang,Qiyuan Liu. Effects of Biochar on Growth, Yield and Quality of Flue-Cured Tobacco [J]. Crops, 2018, 34(2): 154-160.
[8] Liuya Huang,Yongbo Sun,Shuwu Liu,Yonghui Zhang,Fuzhao Nian,Yong Gu. Research Advance of the Effects of Biochar onthe Main Properties of Soil and Yield and Quality of Flue-Cured Tobacco [J]. Crops, 2017, 33(4): 15-20.
[9] Ying Chen,Manli Zhang,Xianping Liu,Guijin Dai,Shougui Hou. Effects of Biochar on Chlorophyll Fluorescence at Full Heading Stage and Yield Components of Rice [J]. Crops, 2016, 32(3): 94-98.
[10] . [J]. Crops, 2013, 29(3): 111-116.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!