Crops ›› 2022, Vol. 38 ›› Issue (4): 138-145.doi: 10.16035/j.issn.1001-7283.2022.04.019

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Effective Effects of Acrylamide/Carboxymethyl Cellulose/Biochar Composite Hydrogel on Cadmium Stress in Tobacco Seedlings

Du Fu1,2(), Xia Maolin1(), Liu Xinyuan3, Yu Zhaojin1, Zhang Zhan4(), Liu Yunfei1, Ji Xiaoming1()   

  1. 1College of Tobacco, Henan Agricultural University, Zhengzhou 450002, Henan, China
    2China Tobacco Hubei Industry Co., Ltd., Wuhan 430040, Hubei, China
    3Technology Center, Sanmenxia Branch of Henan Tobacco Company, Sanmenxia 472400, Henan, China
    4Technology Center, China Tobacco Henan Industry Co., Ltd., Zhengzhou 450008, Henan, China
  • Received:2021-07-15 Revised:2021-09-26 Online:2022-08-15 Published:2022-08-22
  • Contact: Zhang Zhan,Ji Xiaoming E-mail:834681855@qq.com;2359533942@qq.com;zhangzhan2059729@126.com;xiaomingji@henau.edu.cn

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Abstract:

In order to alleviate the toxic effect of cadmium (Cd) on the growth and development of tobacco seedlings, the removal effects of new acrylamide/carboxymethylcellulose/biochar composite hydrogel (AM/CMC/B) on Cd2+ in aqueous solution was investigated, and the effects of AM/CMC/B on alleviating Cd stress of tobacco seedlings was studied by pot experiment. The results showed that AM/CMC/B could effectively remove Cd2+ from water and improve the phenotype of tobacco seedlings under Cd stress. Under AM/CMC/B treatment, the maximum leaf length, maximum leaf width, maximum leaf area, total root length, total root surface area, average root diameter, root tip number and root volume increased significantly by 38.28%, 30.01%, 79.19%, 83.31%, 83.97%, 54.44%, 88.21% and 261.56%, respectively. The fresh and dry weight of aboveground part of tobacco seedlings increased by 98.39% and 84.22%, the fresh and dry weight of underground part of tobacco seedlings increased by 130.67% and 231.82%, respectively. The photosynthetic rate, SPAD value and stomatal conductance of tobacco seedlings significantly increased by 62.03%, 29.61% and 84.42%, the content of malondialdehyde decreased by 36.61%. The antioxidant activity and metabolite content of tobacco seedlings increased. AM/CMC/B could significantly improve the tolerance of tobacco seedlings for Cd stress.

Key words: Carboxymethyl cellulose, Biochar, Hydrogel, Cadmium stress, Tobacco seedlings

Fig.1

FT-IR of AM/CMC/B(a), AM/CMC(b) and B(c)"

Fig.2

Equilibrium adsorption of Cd2+ by different hydrogels under different pH values"

Fig.3

Phenotype of tobacco seedlings under Cd stress for 15 days"

Fig.4

Growth indexes of tobacco seedlings under Cd stress for 15 days Different lowercase letters indicate significant difference at 0.05 level, the same below"

Fig.5

Biomass and leaf RWC of tobacco seedlings under Cd stress for 15 days"

Fig.6

Photosynthetic characteristics and SPAD values of tobacco seedlings under Cd stress for 15 days"

Fig.7

Contents of MDA and Pro, activities of CAT and POD in tobacco seedlings under Cd stress for 15 days"

Table 1

Contents of metabolites in tobacco seedlings under different treatments"

代谢物类别
Metabolite category		 代谢物名称
Metabolite name		 保留时间
Retention time (min)		 含量Content (mg/g)
CK H BH
有机酸Organic acid 苹果酸 5.26 0.255 0.349 0.359
对苯二甲酸 6.91 0.151 0.206 1.407
二十碳五烯酸 8.58 0.000 3.115 0.226
棕榈酸 8.79 1.353 1.871 2.586
亚油酸 10.80 0.239 0.380 0.792
亚麻酸 10.91 1.288 2.221 5.113
硬脂酸 11.26 0.547 0.729 0.993
有机酸总量 3.833 8.872 11.476
萜类Terpenoids 植醇 7.51 0.438 0.610 1.005
α-西柏三烯二醇 11.37 1.838 3.696 4.470
β-西柏三烯二醇 12.28 0.851 1.032 1.274
菜油甾醇 23.86 0.433 0.860 1.574
豆甾醇 24.07 1.563 2.192 3.504
β-谷甾醇 24.47 0.578 1.147 1.763
β-香树脂醇 24.59 0.297 0.540 0.904
鹅去氧胆酸 24.96 0.000 0.000 0.196
萜类总量 5.998 10.076 14.691
烷烃类Alkanes 烷烃Ⅰ 21.34 0.000 0.136 0.258
烷烃Ⅱ 21.60 0.000 0.133 0.197
烷烃Ⅲ 22.07 0.203 0.358 0.729
烷烃Ⅳ 22.22 0.102 0.155 0.301
烷烃Ⅴ 22.59 0.375 0.688 1.314
烷烃Ⅵ 22.79 0.689 0.902 1.538
烷烃Ⅶ 22.99 0.210 0.440 0.519
烷烃Ⅷ 23.13 0.121 0.329 0.461
烷烃Ⅸ 23.72 0.258 0.597 1.163
烷烃Ⅹ 23.96 0.267 0.813 1.240
烷烃总量 2.225 4.550 7.719
其他代谢物Other metabolites 丁基化羟基甲苯 6.51 0.134 0.203 0.258
新植二烯 7.23 3.753 3.423 3.214
十八醇 10.00 0.000 0.000 0.128
4,4'-亚甲基双（2-叔丁基-6-甲基苯酚） 17.82 3.076 3.610 4.691
单硬脂酸甘油酯 20.90 0.354 0.725 1.049
其他代谢物总量 7.317 7.961 9.341
代谢物总量Total metabolites 19.373 31.458 43.227
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