Crops ›› 2025, Vol. 41 ›› Issue (2): 74-78.doi: 10.16035/j.issn.1001-7283.2025.02.010

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Genetic Diversity Analysis of 96 Waxy Maize Inbred Lines Based on SNP Chip

Li Wenyue1(), Yu Tao1, Cao Shiliang1, Ma Xuena1, Tang Gui2, Gao Li3, Yang Gengbin1()   

  1. 1Maize Research Institute, Heilongjiang Academy of Agricultural Sciences / Key Laboratory of Maize Biology and Genetics in the Northern Part of Northeast China, Ministry of Agriculture and Rural Affairs, Harbin 150000, Heilongjiang, China
    2Rural Revitalization Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150000, Heilongjiang, China
    3Suihua Branch, Heilongjiang Academy of Agricultural Sciences, Suihua 152000, Heilongjiang, China
  • Received:2024-01-30 Revised:2024-03-30 Online:2025-04-15 Published:2025-04-16

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Abstract:

In recent years, waxy maize has been popular due to its unique texture and has become a favoured agricultural product widespread popularity. However, for waxy maize inbred lines, currently no clear classification has been reported. In order to improve the efficiency of fresh glutinous corn breeding in the Northeast of China, this study utilized the Maize 6H-60K gene chip to conduct genotype analysis on 96 waxy corn inbred lines from the Northeast of China. The results showed that among the 96 inbred lines, the gene diversity detected by 14 312 SNP markers ranged from 0.098 to 0.500, with an average of 0.387. The minimum allele frequency ranged from 0 to 0.5, with an average of 0.380, and the polymorphic information content ranged from 0.094 to 0.375, with an average of 0.306. The 96 materials were ultimately classified into three clusters, group A was the Kennian 1 maternal group, group B was the Xiannuo paternal group, and group C was the Kennian 1 paternal group.

Key words: Waxy maize, SNP, Group division, Genetic analysis

Fig.1

Distribution of genetic distance for 96 waxy maizes"

Fig.2

Minimum allele frequency histogram"

Table 1

Summary of genotypes"

项目Item 数值Numeric
样品数量Sample number 96
标记数量Number of markers 38 693
杂合比例Heterozygous ratio 0.034
最小等位基因频率Minimum allele frequency 0.380
基因多样性Gene diversity 0.387
PIC 0.306

Fig.3

The change curve of ΔK"

Fig.4

Principal component analysis of 96 waxy maize inbred lines"

Fig.5

Clustering analysis of 96 waxy maize inbred lines"

Fig.6

Group genetic structure analysis of 96 waxy maize inbred lines"

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