Crops ›› 2025, Vol. 41 ›› Issue (5): 42-46.doi: 10.16035/j.issn.1001-7283.2025.05.006

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Effect of Melatonin Soaking on Foxtail Millet Germination under Saline-Alkali Stress

Xu Mingli1(), Wu Baichen1, Liu Chang1, Gao Xinhan1, Yin Jiaqi1, He Xue1, Liu Ying1, Yin Zequn1, Miao Xingfen1,2()   

  1. 1 Agricultural College, Heilongjiang Bayi Agricultural University / Heilongjiang Province Key Laboratory of Modern Agricultural Cultivation Technology and Crop Quality Improvement, Daqing 163319, Heilongjiang, China
    2 Key Laboratory of Green and Low-Carbon Agriculture in Northeast Plain, Ministry of Agriculture and Rural Affairs, Daqing 163319, Heilongjiang, China
  • Received:2024-04-24 Revised:2024-05-24 Online:2025-10-15 Published:2025-10-21

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Abstract:

This experiment investigated the effects of melatonin solution soaking on the seed germination of two foxtail millet varieties, the saline-sensitive variety Datong 29 and the saline-tolerant variety Longgu 25, under an 80 mmol/L saline-alkali stress. The saline-alkali solution was a mixture of Na?SO4, NaCl, NaHCO3, and Na?CO3 at a ratio of 4:1:4:1. Seeds were soaked in melatonin solutions at concentrations of 50, 100, 150, and 200 μmol/L. The results showed that compared with single stress treatment, the root and shoot lengths of both foxtail millet varieties initially increased and then decreased with rising melatonin concentrations. For the saline-alkali sensitive variety, Datong 29, the germination rate, germination index, and antioxidant enzyme activities all showed a gradual increasing trend. A concentration of 200 μmol/L melatonin was the most effective in alleviating saline stress for Datong 29. For the saline-tolerant variety, Longgu 25, the germination rate, germination potential, germination index, vigor index, and antioxidant enzyme activities showed a trend of first increasing and then decreasing. A concentration of 150 μmol/L melatonin was most effective in improving its stress tolerance.

Key words: Foxtail millet, Melatonin, Saline-alkali stress, Germination, Antioxidant enzyme

Table 1

Effects of different concentrations of melatonin on germination characteristics of foxtail millet under saline-alkali stress"

品种Cultivar 处理Treatment 发芽势Germination potential (%) 发芽率Germination rate (%) 发芽指数GI 活力指数VI
大同29
Datong 29		 CK 36.00±1.63a 72.00±1.63a 34.85±0.76a 261.71±5.68a
D 20.00±1.63b 36.67±2.49d 15.77±0.42d 14.98±0.40d
D+50 20.67±0.94b 38.00±1.63d 18.14±0.92c 23.40±1.19c
D+100 21.33±0.94b 43.33±2.49c 18.74±1.10c 24.36±1.43bc
D+150 23.33±2.49b 50.00±1.63b 20.12±0.98bc 30.58±1.49b
D+200 21.33±1.89b 53.33±2.49b 21.56±1.16b 29.97±1.61b
龙谷25
Longgu 25		 CK 54.67±0.94c 85.33±1.89a 42.98±0.76b 326.25±5.75a
D 33.33±1.89e 58.00±2.83d 25.49±1.02d 32.37±1.29f
D+50 48.67±0.94d 68.67±1.89c 35.85±0.82c 46.60±1.07e
D+100 58.00±1.63b 76.67±0.94b 44.16±1.34b 58.73±1.79d
D+150 65.33±0.94a 77.33±0.94b 48.83±1.32a 119.16±3.23b
D+200 60.67±0.94b 76.00±1.63b 47.60±0.85a 69.50±1.23c

Table 2

Effects of different concentrations of melatonin on root length and shoot length of foxtail millet under saline-alkali stress cm"

品种
Cultivar		 CK D D+50 D+100 D+150 D+200
根长
Root
length		 芽长
Bud
length		 根长
Root
length		 芽长
Bud
length		 根长
Root
length		 芽长
Bud
length		 根长
Root
length		 芽长
Bud
length		 根长
Root
length		 芽长
Bud
length		 根长
Root
length		 芽长
Bud
length
大同29 Datong 29 7.51a 2.29a 0.95c 1.21d 1.29b 1.50c 1.30b 1.65bc 1.52b 1.72b 1.39b 1.55c
龙谷25 Longgu 25 7.59a 3.13a 1.27c 1.40c 1.30c 1.51c 1.33c 1.63c 2.44b 2.12b 1.46c 1.62c

Fig.1

Effects of different concentrations of melatonin on antioxidant enzyme activity in foxtail millet under saline-alkali stress Different lowercase letters indicate significant difference among treatments (P < 0.05), the same below."

Table 3

Correlation analysis of the effects of different concentrations of melatonin soaking on the growth and antioxidant enzyme activity of foxtail millet"

指标
Index		 发芽势
Germination potential		 发芽率
Germination rate		 发芽指数
GI		 活力指数
VI		 根长
Root length		 芽长
Bud length		 SOD POD CAT
发芽势Germination potential 1.000
发芽率Germination rate 0.939** 1.000
发芽指数GI 0.994** 0.961** 1.000
活力指数VI 0.450 0.597* 0.507 1.000
根长Root length 0.252 0.434 0.320 0.966** 1.000
芽长Bud length 0.441 0.570 0.495 0.933** 0.859** 1.000
SOD 0.197 0.065 0.166 -0.643* -0.766** -0.437 1.000
POD 0.596* 0.467 0.574 -0.313 -0.505 -0.161 0.879** 1.000
CAT 0.501 0.361 0.483 -0.396 -0.549 -0.235 0.905** 0.954** 1.000
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