Crops ›› 2025, Vol. 41 ›› Issue (5): 67-73.doi: 10.16035/j.issn.1001-7283.2025.05.010

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Effects of Saline-Alkali Stress on Seed Germination of Urtica dioica L.

Wang Yanwei1(), Wu Junxi2(), Wang Yan3, Mu Tao3, Langzhuoma 1, Miao Yanjun1()   

  1. 1 College of Plant Science, Tibet Agricultural and Animal Husbandry University, Linzhi 860000, Tibet, China
    2 Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China
    3 Lhasa Plateau Biology Institute, Lhasa 850008, Tibet, China
  • Received:2024-08-16 Revised:2024-10-09 Online:2025-10-15 Published:2025-10-21

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Abstract:

To investigate the saline-alkali tolerance of Urtica dioica L., a germination assay was conducted under controlled conditions using seeds subjected to saline-alkali stress. Treatments consisted of seven concentrations (0 [CK], 10, 20, 30, 40, 50, and 60 mmol/L) of neutral salts (NaCl, Na2SO4) and an alkaline salt (NaHCO3) in a controlled temperature incubator for seed germination test. Following stress exposure, we assessed the germination rate, germination potential, germination index, and relative salt damage rate, analyzing the impact of saline-alkali stress on U.dioica L. germination. The results showed that under the stress of alkaline salt (NaHCO3), as the stress concentration increased from 10 mmol/L to 60 mmol/L, the seed germination rate decreased from 88.00% to 26.67%, both of which were significantly higher than the seed germination rates under NaCl and Na2SO4 treatments. When the alkaline salt (NaHCO3) was 30, 40 and 60 mmol/L, the seed germination potentials were 45.33%, 35.33% and 17.33%, respectively, which were all significantly higher than those treated with NaCl and Na2SO4. Under the stress of alkaline salt (NaHCO3), as the stress concentration increased from 10 mmol/L to 60 mmol/L, the germination index of U.dioica L. decreased from 35.29% to 8.80%, both of which were significantly higher than the seed germination index under the treatments of NaCl and Na2SO4. The relative salt damage rate of seeds increased from 5.63% to 71.47%, both of which were significantly lower than the relative salt damage rates of seeds under NaCl and Na2SO4 treatments. Membership function analysis indicated the salt tolerance of U.dioica L. seeds at the germination stage follows NaHCO3 > Na2SO4 > NaCl. With the increase of saline-alkali concentrations, seed germination index declined, while the relative salt damage rate increased. Neutral salts demonstrate greater inhibitory effects on seed germination than the alkaline salt, suggesting a better suitability for U.dioica L. cultivation in alkaline soil.

Key words: Urtica dioica L., Saline stress, Alkali stress, Seed germination, Membership function

Fig.1

Effects of saline-alkali stress on germination rate and relative germination rate of U.dioica L. seed Different lowercase letters indicate significant difference among treatments of the same solution (P < 0.05); different capital letters indicate significant difference among different solutions of the same concentration (P < 0.05). The same below."

Fig.2

Effects of saline-alkali stress on germination potential and relative germination potential of U.dioica L. seed"

Fig.3

Effects of saline-alkali stress on germination index and relative germination index of U.dioica L. seed"

Fig.4

Effects of saline-alkali stress on the relative salt damage rate of U.dioica L. seed"

Table 1

Salt tolerance index membership function value and its comprehensive evaluation"

盐处理
Salt
treatment		 发芽率
Germination
rate		 发芽势
Germination
potential		 发芽指数
Germination
index		 相对发芽率
Relative
germination rate		 相对发芽势
Relative germination
potential		 相对发芽指数
Relative
germination index		 平均值
Average
value		 排名
Ranking
NaCl 0.47 0.37 0.40 0.48 0.39 0.52 0.44 3
NaHCO3 0.56 0.43 0.53 0.58 0.46 0.54 0.52 1
Na2SO4 0.47 0.47 0.49 0.49 0.50 0.37 0.45 2
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