Crops ›› 2024, Vol. 40 ›› Issue (6): 212-217.doi: 10.16035/j.issn.1001-7283.2024.06.028

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Effects of Exogenous Silicon on Seed Germination and Physiological Characteristics of Brassica pekinensis under Salt Stress

E Lifeng1,2(), Xu Jinchong1, Chen Xiubin1,2, Quan Jianhua1,2, Hua Jun3, Yin Lijuan4, Wang Shunqi4, Zhao Wenqin5   

  1. 1College of Agriculture and Ecological Engineering, Hexi University, Zhangye 734000, Gansu, China
    2Hexi Corridor Precision Facility Horticulture Engineering and Technology Research Center, Hexi University, Zhangye 734000, Gansu, China
    3Zhangye Economic Crops Technical Extension Station, Zhangye 734000, Gansu, China
    4Linze County Agricultural Technology Extension Center, Linze 734200, Gansu, China
    5Gansu Huamei Agricultural Science and Technology Company Limited, Zhangye 734000, Gansu, China
  • Received:2024-03-19 Revised:2024-05-12 Online:2024-12-15 Published:2024-12-05

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Abstract:

In order to explore the effects of different concentrations of exogenous silicon on seed germination and seedling physiological characteristics of Brassica pekinensis under salt stress, the variety “Huanai B1102 Chunyuhuang” was used as the test material, a single-factor completely randomized block design was conducted. Under 85.55 mmol/L NaCl salt stress, six treatments were set up: CK1 (distilled water), CK2 (NaCl), A1 (NaCl+ 0.5 mmol/L Si), A2 (NaCl+1.0 mmol/L Si), A3 (NaCl+2.0 mmol/L Si), and A4 (NaCl+4.0 mmol/L Si), each treatment was repeated three times. The changes of seed germination, seedling growth index and physiological activity were determined. The results showed that different concentrations of exogenous silicon could effectively alleviate the damage caused by salt stress to the seeds and seedlings of Brassica pekinensis. When the concentration of silicon was 1.0 mmol/L, the germination rate, germination potential, germination index and vigor index of Brassica pekinensis seeds were significantly increased. At the same time, the plant height, stem diameter, leaf area, root volume and chlorophyll content of Brassica pekinensis seedlings also increased significantly, the activity of peroxidase in leaves increased, and the content of malondialdehyde decreased. Exogenous silicon could effectively regulate the inhibition of salt stress on seed germination and seedling growth of “Huanai B1102 Chunyuhuang”, and the best concentration of silicon to alleviate salt stress was 1.0 mmol/L.

Key words: Brassica pekinensis, Exogenous silicon, Salt stress, Seed germination, Seedling growth

Table 1

Effects of different treatments on germination rate, germination potential, germination index and vigor index of Brassica pekinensis seeds"

处理
Treatment		 发芽率
Germination rate (%)		 发芽势
Germination potential (%)		 发芽指数
Germination index		 活力指数
Vigor index
CK1 96.67±1.76a 68.67±0.67a 41.95±0.93a 2.80±0.14a
CK2 86.67±1.76c 39.33±3.53c 31.74±1.36c 1.19±0.05c
A1 92.67±1.76ab 42.00±1.15bc 34.14±0.87bc 1.93±0.22b
A2 94.00±1.15ab 46.67±0.67b 35.66±0.49b 2.28±0.10b
A3 93.33±1.76ab 44.67±0.67bc 35.14±0.38b 1.96±0.08b
A4 89.33±1.76c 41.33±1.33bc 33.90±0.14bc 1.41±0.02c

Table 2

Effects of different treatments on plant height, stem diameter, leaf area and fresh weight of Brassica pekinensis seedlings"

处理Treatment 株高Plant height (cm) 茎粗Stem diameter (mm) 叶面积Leaf area (cm2) 鲜重Fresh weight (g)
CK1 16.79±0.53a 4.13±0.19a 126.41±1.62a 14.33±1.18a
CK2 11.70±0.99bc 2.63±0.12b 63.86±3.60c 9.53±0.16c
A1 13.28±0.40b 3.60±0.15a 83.78±3.06c 9.46±0.25c
A2 16.64±0.56a 3.77±0.18a 111.62±2.31b 11.06±0.41bc
A3 12.70±0.40b 3.57±0.24a 60.89±1.98d 11.90±0.64b
A4 10.25±0.35c 2.80±0.20b 60.28±3.57d 9.88±0.46c

Table 3

Effects of different treatments on total root length, root surface area, root volume and root tip number of Brassica pekinensis seedlings"

处理Treatment 根系总长Total root length (cm) 根系表面积Root surface area (cm2) 根系体积Root volume (cm3) 根尖数Number of root tips
CK1 135.65±20.40a 353.98±9.55a 49.31±15.11a 135.00±20.31a
CK2 73.42±13.75c 175.53±13.74c 28.08±7.96a 38.67±4.91bc
A1 121.01±4.46ab 264.19±7.16b 36.21±8.33a 67.00±0.00bc
A2 135.34±13.97a 352.86±22.93a 49.04±13.99a 148.33±12.02a
A3 83.72±4.65bc 241.70±14.33b 39.23±7.33a 69.33±11.20b
A4 109.59±10.29abc 150.04±15.25c 25.99±9.37a 32.00±1.00c

Fig.1

Effects of different treatments on the morphology and root system of Brassica pekinensis seedlings"

Table 4

Effects of different treatments on contents of chlorophyll a, chlorophyll b, carotenoids and total chlorophyll (a+b) of Brassica pekinensis leaves mg/g"

处理
Treatment		 叶绿素a
Chlorophyll a		 叶绿素b
Chlorophyll b		 类胡萝卜素
Carotenoids		 叶绿素（a+b）
Chlorophyll (a+b)
CK1 9.13±0.06a 5.78±0.11a 1.26±0.03a 14.91±0.16a
CK2 4.02±0.07c 1.66±0.11c 0.93±0.02b 5.67±0.18c
A1 5.81±0.06b 3.91±0.07b 0.68±0.01bc 9.72±0.13b
A2 5.66±0.05b 4.13±0.69b 0.64±0.20c 9.79±0.73b
A3 3.94±0.05c 1.77±0.09c 0.92±0.02b 5.72±0.14c
A4 3.03±0.08d 1.81±0.19c 0.54±0.04c 4.84±0.27c

Fig.2

Effects of different treatments on POD activity and MDA content of Brassica pekinensis seedlings Different lowercase letters indicate significant difference at the P < 0.05 level."

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