国家甘薯普查资源徐州圃病毒种类鉴定与分析

doi:10.16035/j.issn.1001-7283.2025.06.032

Abstract

Abstract:

The total of 212 suspected virus-infected sweetpotato samples were collected from the Xuzhou nursery of the national sweetpotato resources collection, and the samples were screened for viruses using RT-PCR with 19 pairs of sweetpotato virus-specific primers. The virus detection rate was 100% in 212 suspected samples. There were 12 species of viruses were detected; nine species were RNA viruses. Among these RNA viruses, the detection rate of sweetpotato feathery mottle virus (SPFMV) was the highest at 75.47%, and the detection rate of cucumber mosaic virus (CMV) was the lowest at 0.94%. We detected three types of DNA viruses, namely sweetpotato leaf curl virus (SPLCV), sweetpotato pakakuy virus (SPPV), and sweetpotato symptomless virus 1 (SPSMV-1). Among the DNA viruses, the detection rate of SPPV was the highest at 80.66%, and the detection rate of SPLCV was the lowest at 47.75%. There were 206 samples co-infected by different viruses, accounting for 97.17% of all samples. There were 156 co-infection types, and 123 of them included only one sample. In the Xuzhou nursery of the national sweetpotato resources, a wide variety of viruses infecting sweetpotatoes were identified, with high infection rates and widespread complex mixed infections.

Key words: Sweetpotato, Collection resources, Virus disease, RT-PCR

Tang Wei, Yuan Rui, Zhang Chengling, Zhao Lukuan, Ma Jukui, Chen Jingwei, Yang Dongjing, Gao Fangyuan, Zhou Zhilin, Sun Houjun. Virus Identification and Analysis in National Sweetpotato Collection Resources from the Xuzhou Nursery[J].Crops, 2025, 41(6): 254-262.

Figures/Tables 6

Table 1

The information of 212 sweetpotato samples"

序号 Number	采集编号 Collection number	来源地 Origin	序号 Number	采集编号 Collection number	来源地 Origin	序号 Number	采集编号 Collection number	来源地 Origin	序号 Number	采集编号 Collection number		来源地 Origin
1	P341302008	安徽	36	P350921006	福建	71	2018461034	海南	106	P430302133		湖南
2	P341523025	安徽	37	2018351310	福建	72	P231086037	黑龙江	107	P430781007		湖南
3	P340221025	安徽	38	P350213015	福建	73	2015422023	湖北	108	2021431027		湖南
4	P340827035	安徽	39	P350921005	福建	74	2018422006	湖北	109	2021431029		湖南
5	2021342623	安徽	40	P350824108	福建	75	2018423129	湖北	110	2022432083		湖南
6	P340122049	安徽	41	P350128089	福建	76	2015422160	湖北	111	2022432133		湖南
7	2019345087	安徽	42	P350128096	福建	77	2015422088	湖北	112	2021432441		湖南
8	2019342038	安徽	43	2021353038	福建	78	2015421284	湖北	113	2021432443		湖南
9	P341802019	安徽	44	2021353039	福建	79	2018423135	湖北	114	P431002035		湖南
10	2019344109	安徽	45	2021353040	福建	80	2015421234	湖北	115	P431122023		湖南
11	P340722004	安徽	46	2015453351	广西	81	2015421283	湖北	116	2017323024		江苏
12	P340824056	安徽	47	2015452303	广西	82	2015421185	湖北	117	2017323025		江苏
13	P341825012	安徽	48	2015452320	广西	83	2015421190	湖北	118	2017323436		江苏
14	P341722015	安徽	49	P450721010	广西	84	2015421050	湖北	119	2016323035		江苏
15	P342726289	安徽	50	2015453289	广西	85	2016423360	湖北	120	2018323605		江苏
16	2021344014	安徽	51	2016452651	广西	86	2015422132	湖北	121	p320684003		江苏
17	2020344027	安徽	52	2016452945	广西	87	2016431283	湖南	122	p320211053		江苏
18	P341221030	安徽	53	2015453332	广西	88	P430525004	湖南	123	P321281006		江苏
19	2020342036	安徽	54	2016453502	广西	89	2016432162	湖南	124	2017323822		江苏
20	P110114043	北京	55	2016452628	广西	90	2016432216	湖南	125	2016322534		江苏
21	P110114042	北京	56	2016452648	广西	91	P431122025	湖南	126	2016321476		江苏
22	2017354088	福建	57	2016453008	广西	92	2016432618	湖南	127	2017323437		江苏
23	2017355015	福建	58	2021521652	贵州	93	2016432357	湖南	128	2016322186		江苏
24	2017355058	福建	59	P520302005	贵州	94	2016432443	湖南	129	P320116027		江苏
25	2018351040	福建	60	2022522305	贵州	95	2016431913	湖南	130	P320584007		江苏
26	P350403120	福建	61	2021522102	贵州	96	2015431136	湖南	131	P320582007		江苏
27	P350128086	福建	62	2022522107	贵州	97	2016431207	湖南	132	2020321740		江苏
28	P350626108	福建	63	2017461053	海南	98	2016432844	湖南	133	P320621009		江苏
29	P350505015	福建	64	p460200021	海南	99	P431122017	湖南	134	2017363098		江西
30	P350582112	福建	65	p460200022	海南	100	P431122024	湖南	135	P360281024		江西
31	2021353034	福建	66	p469005026	海南	101	P430221021	湖南	136	2018362382		江西
32	2021353033	福建	67	p469002007	海南	102	P430225013	湖南	137	2017361341		江西
33	P350212030	福建	68	2019461025	海南	103	P430321004	湖南	138	P360924013		江西
34	P350425015	福建	69	2017461014	海南	104	P430321013	湖南	139	2018361547		江西
35	P350781112	福建	70	2018461002	海南	105	P433101040	湖南	140	2018363465		江西
序号 Number	采集编号 Collection number	来源地 Origin	序号 Number	采集编号 Collection number	来源地 Origin	序号 Number	采集编号 Collection number	来源地 Origin	序号 Number		采集编号 Collection number	来源地 Origin
141	2018362346	江西	159	P371703041	山东	177	2021616166	陕西	195		2018331096	浙江
142	P211204025	辽宁	160	P371083130	山东	178	P511133023	四川	196		2017332084	浙江
143	2018361440	江西	161	P371083131	山东	179	P510503016	四川	197		2017331056	浙江
144	P360828015	江西	162	P371722030	山东	180	2020512050	四川	198		2017331106	浙江
145	2018361304	江西	163	P370212020	山东	181	2019516120	四川	199		P330324024	浙江
146	P363321026	江西	164	2021373174	山东	182	2020513010	四川	200		2017332085	浙江
147	2017363070	江西	165	P371082102	山东	183	P532502033	云南	201		P330784022	浙江
148	2017361354	江西	166	2022313864	山东	184	P530325033	云南	202		P330683033	浙江
149	2019363058	江西	167	2022373865	山东	185	P530325034	云南	203		2017362018	江西
150	P210282063	辽宁	168	2022373874	山东	186	2021532200	云南	204		2018362126	江西
151	2018361607	江西	169	2022373882	山东	187	2021532508	云南	205		2019361001	江西
152	P360423049	江西	170	2022373029	山东	188	2022533671	云南	206		P530521028	云南
153	P361102010	江西	171	2022373037	山东	189	2022533645	云南	207		P533122038	云南
154	P360922061	江西	172	P140481014	山西	190	2018334452	浙江	208		P530128069	云南
155	P370902056	山东	173	P141028004	山西	191	2018334285	浙江	209		2022522993	贵州
156	P370215041	山东	174	P610630001	陕西	192	2018335245	浙江	210		2022525001	贵州
157	P371121028	山东	175	P610621004	陕西	193	P331022010	浙江	211		2022525004	贵州
158	P371083134	山东	176	2020615300	陕西	194	2018334102	浙江	212		2022525009	贵州

Table 1

Table 2

Fig.1

Fig.2

Fig.3

Fig.4

References 35

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病毒名称Virus name	属Genus	引物（5′→3′）Primer (5′→3′)		片段大小Fragment size (bp)	参考文献Reference
SPC6V	Carlavirus	F	AAAAGCTTGTTGGCAATTTGTG	590	[15]
		R	TTGGCATTTCGATTGTCCC
SPCV	Cavemovirus	F	AGGAAATCCCAGTATTATTCAAC	927	[16]
		R	ATTTCTAATTTGGTTTACTAATCC
SPVCV	Solendovirus	F	TGAATGCAAAGACAAAAACCTA	373	[17]
		R	GATAAACTAACTCCTGCTTCTT
SPVF	Carlavirus	F	ATGTTTCCTGGAGAAAGTCAAGAAAGC	930	[18]
		R	AGGCTTAATAGCTGCATGGTTAGTCACA
SPLV	Potyvirus	F	CCAAATATCAATGGTGATTGGGT	420	[19]
		R	CCACGCATTCCAAGTAGTGTGTGT
CMV	Cucumovirus	F	ATGGACAAATCTRAATCAACCAG	657	[20]
		R	TCARACTGGGAGCACYCCWGAYGT
SPCFV	Carlavirus	F	CTATGCTGCTCACTCAAGC	600	[21]
		R	TTGATTGGCCACAAGCGAAG
SPMMV	Ipomovirus	F	CAAAGCTGGACAAGGAGCG	700	[12]
	Ipomovirus	R	ACAATCCATGCTGGCAGTACT
TMV	Tobamovirus	F	TAGACCCGCTAGTCACAG	237	[22]
		R	CAGAGGTCCAAACCAAAC
SPMSV	Potyvirus	F	AAGACAAAGGTAAAACTGTGGC	941	[13]
		R	ACATACTCGTATTAAAACACATAAG
SPCSV	Crivirus	F	GGGAAGAMGAGAYATGGAGTTAA	431	[13]
		R	CCTTGTTACAAAGAGCGTTCCT
SPSMV-1	Mastrevirus	F	GGCGTTTCTACCTGGGATGATG	394	本文
		R	ACACATCTCTTGCAACAGTCCAC
SPPV	Badnavirus	F	GCAGCAGTCACAGAAGTCG	603	本文
		R	CATRATATTYTGGCAGAAATC
SPLCV	Begomovirus	F	TAATTCGAACTGCAGTTCCGTATTTACAGTT	514	[23]
		R	GCTAGAGGAGGCCTGCAGACTGCTAACGACG
		R	TCGGGACTGAARGAYACGAATTTAA		[24]
SPFMV	Potyvirus	F	GGATTAYGGTGTTGACGACACA	589	[24]
SPV2	Potyvirus	F	CGTACATTGAAAAGAGAAACAGGATA	369	[24]
SPVC	Potyvirus	F	GTGAGAAAYCTATGCGCTCTGTT	836	[24]
SPVG	Potyvirus	F	GTATGAAGACTCTCTGACAAATTTTG	1100	[24]
SPVE	Potyvirus	F	CAATTCGACAACACCCGCT	278	[25]

Virus Identification and Analysis in National Sweetpotato Collection Resources from the Xuzhou Nursery

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