Crops ›› 2016, Vol. 32 ›› Issue (4): 41-46.doi: 10.16035/j.issn.1001-7283.2016.04.007

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Identification and Gene Mapping of a Yellow-Leaf Rice Mutant ys94

Ming Ming,Wang Ying,Lin Qibing,Wang Yihua,Cheng Zhijun   

  1. Institute of Crop Science, Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Received:2016-04-01 Revised:2016-06-14 Online:2016-08-15 Published:2018-08-26
  • Contact: Zhijun Cheng

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Abstract:

Abstrate A yellow-leaf mutant ys94 was isolated by chemical mutagenesis. Compared with its wild type, ys94 mutant displayed yellow leaf phenotype and reduced contents of both chlorophyll a and chlorophyll b in the germination period. The results of electron microscopic observation revealed that there were significantly reduced number of chloroplast,chloroplast dysplasia and degenerated thylakoid lamella in the ys94 in compared to WT.The tiller number of the mutant was reduced,but the plant height,the number of spikelets per panicle and the seed setting had no significant difference with the wild type. Genetic analysis showed that the mutation of ys94 was controlled by one recessive nuclear gene. Map-based mapping of the mutant gene was conducted by using of the F2population from ys94×DJY. The mutant gene was finally mapped to a 140kb region between the maker M8-3 and M8-24 on the short arm of chromosome 8, in which there were 11 annotated genes. Our study results have set a foundation for future cloning and functional analysis of the gene.

Key words: Key words Rice, Leaf-colour mutant, Gene mapping, Yellow leaf, ys94

Table 1

Agronomic traits of the WT and mutant"

农艺性状Agronomic traits 野生型Wild type 突变体ys94 比对照增减Compared with WT(%)
株高Plant height(cm) 103.03±3.36 102.53±2.91 -0.49
分蘖数Tiller number(个) 9.89±1.69 8.91±1.42 -9.90*
结实率Seed-setting rate(%) 82.93±1.71 84.41±1.30 1.70
有效分蘖数Effective tiller number(个) 9.26±1.60 8.66±1.36 -6.50*
每穗粒数No. of spikelets per panicle(粒) 102.77±20.23 103.18±22.78 0.40
千粒重1000-kernel weight(g) 24.41±0.03 25.11±0.04 2.90

Fig.1

Comparison of photosynthetic pigments contents between the WT and the ys94 mutant Different letters indicate significant difference at P=0.01 level"

Fig.2

Ultrastructures of the development of chloroplasts in the WT and ys94 mutant A: Overview of chloroplasts in WT plants; B: Overview of chloroplasts in mutant plants; C: Ultrastructure of the chloroplast in WT plants; D: Ultrastructure of the chloroplast in mutant plants"

Fig.3

Expression levels of genes associated with pigment metabolism and photosynthesis in WT and ys94"

Fig.4

Fine mapping of target gene The target gene was located in the interval of 140kb delimited by markers M8-3 and M8-24 using 2216 F2 mutants"

Fig.5

Sequence analysis of the candidate gene LOC_Os08g12840 in WT and mutant"

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