Crops ›› 2017, Vol. 33 ›› Issue (3): 60-68.doi: 10.16035/j.issn.1001-7283.2017.03.012

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Effects of Exogenous Salicylic Acid on Cell Ultrastructure of Winter Wheat under Cold Stress in Frigid Region

Liu Lijie1,Zhang Dongxiang1,Jin Zhongmin1,Lin Lidong1,Ren Yanbo1,Zhao Jingtong1,Cang Jing2   

  1. 1 College of Life Science and Agriculture Forestry,Qiqihar University,Qiqihar 161006,Heilongjiang,China
    2 College of Life Science,Northeast Agricultural University,Harbin 150030,Heilongjiang,China
  • Received:2017-03-07 Revised:2017-04-26 Online:2017-06-15 Published:2018-08-26
  • Contact: Jing Cang

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Abstract:

In order to investigate the effects of exogenous salicylic acid (SA) on cell ultrastructure of winter wheat under low temperature, Dongnongdongmai No.1 (the winter wheat cultivar in frigid region) was used as material and 1×10 -4 mol/L SA was sprayed at seedlings growing to three leaves stages using spraying distilled water as control. In the winter natural cooling conditions, tillering nodes and stems were sampled, at 5℃, 0℃, -10℃, and -25℃, respectively,while Malondialdehyde (MDA) content was determined and the ultrastructure of the cells were observed by transmission electron microscopy, and the return rate and the number of tillering nodes were counted. The results showed that exogenous SA alleviated the damage caused by low temperature on membrane system. The cells in the control group appeared slight plasmolysis and slight vacuolization, mitochondrial membrane and nuclear membrane was not clear, but there was no damage to the nucleus only at -25℃. After the application of SA, nuclear damage degree was low, the nuclear and mitochondria membrane were clear, the number of cristae was slightly increased, the number of mitochondria increased in tillering nodes, Only at -25℃, nuclear membrane and mitochondrial membrane were not clear, and they were slightly damaged, but lighter than the control group; The number of plastids and starch grains were increased (above 0℃), and the number of vacuoles in tillering nodes was significantly more than that in the control group. The return rate and tillering nodes number were increased after reviving by exogenous SA. This showed that SA pretreatment could enhance the stability of the cell structure at low temperature, so as to improve the adaptability to low temperature of winter wheat in cold region, from the view of cytology it was confirmed the application of suitable concentration of SA could enhance the cold resistance of winter wheat in cold area.

Key words: Winter wheat cultivar in frigid region, Exogenous salicylic acid, Cell ultrastructure

Fig.1

Change of maximum, minimum, and average temperature during the experiment"

Fig.2

Effects of SA on MDA content of tillering nodesof winter wheat cultivar in frigid region"

Fig.3

Effects of SA on MDA content of stems of winter wheat cultivar in frigid region"

Fig.4

The ultrastructure of tillering nodes of winter wheat in frigid region in the control group at 5℃N: Nucleus; M: Mitochondria; V: Vacuole; Pr: Proplastid, the same below"

Fig.5

The ultrastructure of tillering nodes of winter wheat in frigid region in SA treatment group at 5℃"

Fig.6

The ultrastructure of stems of winter wheat in frigid region in the control group at 5℃"

Fig.7

The ultrastructure of stems of winter wheat in frigid region in SA treatment group at 5℃"

Fig.8

The ultrastructure of tillering nodes of winter wheat in frigid region in the control group at 0℃"

Fig.9

The ultrastructure of tillering nodes of winter wheat in frigid region in SA treatment group at 0℃"

Fig.10

The ultrastructure of stems of winter wheat in frigid region in the control group at 0℃"

Fig.11

The ultrastructure of stems of winter wheat in frigid region in SA treatment group at 0℃"

Fig.12

The ultrastructure of tillering nodes of winter wheat in frigid region in the control group at -10℃"

Fig.13

The ultrastructure of tillering nodes of winter wheat in frigid region in SA treatment group at -10℃"

Fig.14

The ultrastructure of stems of winter wheat in frigid region in the control group at -10℃"

Fig.15

The ultrastructure of stems of winter wheat in frigid region in SA treatment group at -10℃"

Fig.16

The ultrastructure of tillering nodes of winter wheat in frigid region in the control group at -25℃"

Fig.17

The ultrastructure of tillering nodes of winter wheat in frigid region in SA treatment group at -25℃"

Fig.18

The ultrastructure of stems of winter wheat in frigid region in the control group at -25℃"

Fig.19

The ultrastructure of stems of winter wheat in frigid region in SA treatment group at -25℃"

Table 1

Investigation of reviving rate"

处理
Treatment		 分蘖节数Number of tillering nodes 返青率(%)
Reviving rate
越冬前Before winter 返青后 After reviving
对照(CK) 3 3 85
SA 3 8 91
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