Crops ›› 2019, Vol. 35 ›› Issue (4): 196-202.doi: 10.16035/j.issn.1001-7283.2019.04.030

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Analysis of Cellulase Activities of Macrophomina phaseolina in Sesame

Gao Shuguang1,2,Xu Bohan2,Zhao Hui1,Ni Yunxia1,Li Weifeng2,Wang Ruixia2,Xu Dongyang2,Yang Guangyu2,Liu Hongyan1   

  1. 1 Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China
    2 Zhoukou Academy of Agricultural Sciences, Zhoukou 466001, Henan, China
  • Received:2019-03-11 Revised:2019-07-10 Online:2019-08-15 Published:2019-08-06
  • Contact: Hongyan Liu

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Abstract:

To determine the types and activities of cellulases secreted by Macrophomina phaseolina and explore their roles in pathogenic process, seven strains of M. phaseolina were collected from different regions and studied. The crude enzyme solution was prepared by liquid culture. The activities and change graphs of cellulases in vitro were determined by spectrophotometry at 540nm wavelength. The results showed that the activities of filter paper enzyme, natural cellulase, endoglucanase, exoglucanase and β-glucosidase could be detected in all seven strains, and the enzyme activity of seven strains was different at different sampling time by observing the enzymatic activity curves. There were peaks on the enzyme activity curve but the time of peak value was different in different M. phaseolinas strains. In addition, there were significant differences in the comprehensive enzyme activities. It proved that M. phaseolina could secrete a group of extracellular cellulose and the cellulases could degrade cellulose of sesame straw. It provided an important theoretical basis to study the pathogenic mechanism of M. phaseolina in sesame.

Key words: Sesame, Macrophomina phaseolina, Cellulase

Table 1

The tested strains"

菌株
Strains		 采集年份
Year of collection		 采集地点
Location of collection
2010003 2010 江西省鄱阳县清湖村
Qinghu, Poyang, Jiangxi
2010010 2010 江苏省盱眙县龙泉村
Longquan, Xuyi, Jiangsu
2010028 2010 河南省唐河县赵庄村
Zhaozhuang, Tanghe, Henan
2010032 2010 湖北省襄樊钟岗服务区
Zhonggang Service Area, Xiangfan, Hubei
2010064 2010 河南省平舆县王栋桥庄
Wangdongqiao, Pingyu, Henan
2010082 2010 安徽省临泉县李窑村
Liyao, Linquan, Anhui
G243 2011 辽宁省朝阳县胜利乡
Shengli, Chaoyang, Liaoning

Fig.1

Glucose standard curve"

Fig.2

Filter paper enzyme activity of M. phaseolina"

Fig.3

Natural cellulase activity of M. phaseolina"

Fig.4

Endoglucanase activity of M. phaseolina"

Fig.5

Exoglucanase activity of M. phaseolina"

Fig.6

β-glucosidase activity of M. phaseolina"

Table 2

Comparison of A value of cellulase of M. phaseolina in sesame"

编号Number 2010003 2010010 2010028 2010032 2010064 2010082 G243
滤纸酶Filter paper enzyme 2.214Aa 0.838CDc 1.258Bb 0.973Cc 0.591Dd 2.112Aa 0.973Cc
天然纤维素酶Natural cellulase 2.407Aa 0.870DEde 1.611Cb 1.306Cc 0.707Ee 2.451Aa 1.074CDcd
内切葡聚糖苷酶Endoglucanase 3.614Bb 2.413De 3.017Cc 2.782Cd 2.017Ef 3.947Aa 2.801Ccd
外切葡聚糖苷酶Exoglucanase 0.436Aa 0.108Ccd 0.161Bb 0.140Cbc 0.049De 0.456Aa 0.101Cd
β-葡萄糖苷酶β-glucosidase 5.104Bb 0.379Dd 1.686Cc 0.932CDcd 0.313Dd 12.210Aa 0.626CDd
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