国审4个漯麦品种重要性状功能基因KASP检测与分析

doi:10.16035/j.issn.1001-7283.2024.05.002

Abstract

Abstract:

Four national-approved wheat varieties (Luomai 163, Luomai 47, Luomai 49, and Luomai 36) were examined for the presence of key functional genes, such as photoperiod, grain weight, quality, spike sprouting, drought and disease resistance by using KASP marker detection. The results showed that, the four wheat varieties contained dwarfing gene Rht-D1b, allelic variants TaPRR73-A1 and TaPRR73-B1 related to plant height at maturity, photoperiod sensitive allele Ppd-A1b, photoperiod insensitive allele Ppd-Dla, high 1000-grain weight allele TaGS5-Ala, high grain number excellent variant Hap-7A-3, high quality subunit gene Ax1/Ax2* related to quality, high lipoxygenase activity allele Lox-B1a, anti-sprouting gene Rio Blanco type and TaSdr-B1a, and high lignin content excellent allele COMT-3Ba, all accounting for 100%. The utilization of hard genotype Pinb-D1b, high molecular weight glutenin subunit (5+10), stripe rust resistance gene Yr78 and leaf rust resistance gene Lr16 were higher in the four cultivars. High 1000-grain weight allele Hap-A, good allele variant of grain number per spike TaMoc-7A, grain hardness gene Pina-D1b/Null, Pinb-B2b, drought resistance gene 1-fehw3, powdery mildew resistance gene Pm21, and leaf rust resistance gene Lr46 and Lr48 were not detected. The findings of four significant characteristic functional genes of wheat varieties offered crucial reference information for the practical use of upcoming varieties.

Key words: Wheat, Functional gene, KASP detection, Analysis

Cao Yanyan, Li Leilei, Ge Changbin, Huang Jie, Zhou Luqi, Wang Jun, Yang Senyao, Liao Ping’an. KASP Detection and Analysis of Functional Genes of Important Traits in Four National-Approved Luomai Varieties[J].Crops, 2024, 40(5): 8-17.

Figures/Tables 7

Table 1

Table 2

Details of related genes and markers"

类型 Type	性状 Characteristic	遗传效应 Genetic effect	基因 Gene	标记 Marker	等位变异 Allelic variation	表型 Phenotype
适应性相关性状 Adaptive-related trait	株高	主效	Rht-B1	Rht-B1_SNP	Rht-B1a	野生型
适应性相关性状 Adaptive-related trait					Rht-B1b	矮秆型
	株高	主效	Rht-D1	Rht-D1_SNP	Rht-D1a	野生型
					Rht-D1b	矮秆型
	株高	主效	Rht8	Rht8	Rht8+	含矮秆基因
					Rht8-	不含矮秆基因
	株高、熟期	微效	TaPRR73-A1	PRR73A1-9IND	Hap-I	晚熟，矮
					Hap-II	早熟，高
	株高、熟期	微效	TaPRR73-B1	PRR73B1-4558	Hap-I	早熟，高
					Hap-II	晚熟，矮
	光周期	主效	Ppd-A1	GS105-1117_InDel	Ppd-A1a	光周期迟钝
					Ppd-A1b	光周期敏感
	光周期	主效	Ppd-B1	TaPpdBJ001	Ppd-B1a	光周期迟钝
					Ppd-B1b	光周期敏感
	光周期	主效	Ppd-D1	TaPpdDJ001	Ppd-D1a	光周期迟钝
					Ppd-D1b	光周期敏感
	芒	主效	AWN	Absence	AWN+	有芒
				Presence	AWN-	无芒
产量相关性状 Yield-related trait	千粒重	微效	Sus1-7A	Sus1-7A-1185_SNP	Hap-I	低千粒重
产量相关性状 Yield-related trait					Hap-II	高千粒重
	千粒重	微效	TaGASR7-A1	TaGASR	H1C	高千粒重
					H1G	低千粒重
	千粒重	微效	TaSus2-2A	Sus2-2A-20SNP	Hap-A	高千粒重
					Hap-G	低千粒重
	粒重	微效	TaGS5	GS5_SNP	TaGS5-Ala	大粒，粒重高
					TaGS5-Alb	小粒，粒重低
	穗粒数	微效	TEF-7A	TEF7A-1-bp_IND	Hap-7A-3	粒数较多
					Hap-7A-1/2	粒数较少
	穗粒数	微效	TaMoc-7A	TaMoc-7A_2433	Hap-H	粒数较多
					Hap-L	粒数较少
品质相关性状 Quality-related trait	籽粒硬度	主效	Pina-D1	Pina-D1	Pina-D1a	软质
品质相关性状 Quality-related trait					Pina-D1b/Null	硬质
	籽粒硬度	主效	Pinb-D1	Pinb-D1	Pinb-D1a	软质
					Pinb-D1b	硬质
	籽粒硬度	微效	Pinb2-V	Pinb2-Bv2	Pinb-B2a	软质
					Pinb-B2b	硬质
	面筋强度	主效	Glu-D1	Glu-D1_SNP	2+12	弱筋
					5+10	强筋
	面筋强度	主效	Glu-A1	Glu-A1-13	AxNull	弱筋
					Ax1/Ax2*	强筋
	籽粒蛋白质含量	微效	Gpc	GPC_DUC	Gpc-B1-	含量正常
					Gpc-B1+	含量增加
	籽粒蛋白质含量	微效	NAM-6A	NAM-6A_SNP1	T/A1c/A1d	含量正常
					G/A1a/A1b	含量增加
	黄色素含量	主效	Psy-D1	Psy1Da-g	Psy-D1a	含量低
					Psy-D1g	含量高
	黄色素含量	微效	Zds-A1	ZDS-A1_SNP	Zds-A1a	含量高
					Zds-A1b	含量低
	黄色素含量	微效	TaPds-B1	PDS-B1_SNP	TaPds-B1a	含量高
					TaPds-B1b	含量低
	过氧化物酶表达量	微效	Pod-A1	Pod-A1	TaPod-A1a	酶含量低
					TaPod-A1b	酶含量高
	脂肪氧化酶表达量	主效	Lox-B1	Lox-B1	Lox-B1b	酶含量低
					Lox-B1a	酶含量高
	可溶性糖含量	微效	SST-4D	SST-4D-1093	TaSST-D1a	糖含量高
					TaSST-D1b	糖含量低
抗逆相关性状 Resistance-related trait	抗旱	主效	TaDreb-B1	TaDreb_SNP	TaDreb-B1a	抗旱
抗逆相关性状 Resistance-related trait					TaDreb-B1b	不抗旱
	抗旱	主效	1-fehw3	1-FEH-6B	Kauz type	不抗旱
					Westonia type	抗旱
	穗发芽	主效	TaPHS1	PHS1-646	NW97S186 type	感穗发芽
					Rio Blanco type	抗穗发芽
	穗发芽	微效	Sdr-B1	Sdr-B1	TaSdr-B1b	感穗发芽
					TaSdr-B1a	抗穗发芽
	木质素含量	微效	COMT-3B	COMT3B_882_SNP	COMT-3Ba	木质素含量高
					COMT-3Bb	木质素含量低
	抗白粉病	主效	Pm21	Pm21_SNP	Pm21-	感病
					Pm21+	抗病
	抗赤霉病	主效	Fhb1	Fhb1_KSU	Fhb1-	感病
					Fhb1+	抗病
	抗条锈病	微效	Yr78	IWA7257	Yr78-	感病
					Yr78+	抗病
	抗叶锈病	微效	Lr46	Lr46_JF2-2A	Lr46-	感病
					Lr46+	抗病
	抗叶锈病	微效	Lr16	kwm847	Lr16-	感病
					Lr16+	抗病
	抗叶锈病	微效	Lr16	kwm849	Lr16-	感病
					Lr16+	抗病
	抗叶锈病	微效	Lr48	IWB70147	Lr48-	感病
					Lr48+	抗病

Table 2

Fig.1

Table 3

Table 4

Table 5

Table 6

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品种Cultivar	育成年份Year of release	系谱Pedigree
漯麦163 Luomai 163	2018鄂审/2020国审	漯麦6010/弗罗里达
漯麦36 Luomai 36	2022国审	郑麦7698/漯麦6010
漯麦49 Luomai 49	2022国审	漯麦6010/郑麦9023
漯麦47 Luomai 47	2021鄂审/2023国审	漯麦6010/一粒葡

KASP Detection and Analysis of Functional Genes of Important Traits in Four National-Approved Luomai Varieties

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