Crops ›› 2026, Vol. 42 ›› Issue (1): 111-117.doi: 10.16035/j.issn.1001-7283.2026.01.014

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Effects of Drought and Re-Watering on Reactive Oxygen Species Metabolism and Endogenous Hormones in Sweetpotato Roots during Tuberous Root Differentiation and Formation Stage

Wu Hao(), He Jinping, Liao Zhaoxia, Xue Chengkang, Wu Yaoyao, Li Zongyun, Liu Jingran()   

  1. School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, Jiangsu, China
  • Received:2024-06-20 Revised:2024-08-14 Online:2026-02-15 Published:2026-02-10

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Abstract:

Drought experiments were carried out in different stages of tuberous root differentiation and formation by potting soil cultivation to analyze the changes of reactive oxygen species metabolism and endogenous hormone content in sweetpotato roots. The results showed that drought stress caused the decrease of sweetpotato fresh tuber weight per plant, and the earlier the drought, the greater the effect. Compared with normal irrigation treatment (CK), the O2-. content in sweetpotato roots was significantly decreased and H2O2 content was significantly increased under drought stress. The increase of H2O2 was effectively inhibited after rehydration. Drought stress at different periods resulted in the decrease of activities of peroxidase (POD) and catalase (CAT) in sweetpotato roots, and ascorbate peroxidase (APX) activity increased significantly in early, middle and late stages of sweetpotato root differentiation and formation under drought stress. The results showed that the root system of sweetpotato mainly relied on APX to remove reactive oxygen species under drought stress, and SOD also made great contribution to the removal of reactive oxygen species during the early and middle stages of drought stress. After rehydration, the APX activity in sweetpotato roots decreased, while POD activity increased, indicating that sweetpotato root recovered the damage caused by drought stress by enhancing POD activity to improve antioxidant capacity after rehydration. In addition, the free proline content in sweetpotato roots increased under drought stress, and as an osmotic regulator to resist drought stress, the proline content in sweetpotato roots decreased after rehydration. Compared with CK, the contents of ABA and JA in sweetpotato roots for the treatments of D8-14 and D15-21 were significantly increased, while the contents of GA and IAA were significantly decreased, and the decreasing ranges were smaller than the increasing ranges of ABA and JA. When drought treatment (D22-28) was carried out at late stage of root differentiation and formation, the contents of these four endogenous hormones in sweetpotato roots did not change significantly. These results indicated that when sweetpotato was subjected to drought stress in early and middle stages of tuberous root differentiation and formation, it responded strongly by increasing the contents of JA and ABA in roots. However, when sweetpotato was subjected to drought stress during tuberous root differentiation and formation stage, only JA had a significant increase.

Key words: Sweetpotato, Drought, Tuber differentiation and formation, Endogenous hormone, Reactive oxygen species metabolism

Fig.1

Drought treatment marker This phase is treated with drought for seven days."

Fig.2

Effects of drought stress at different stages on fresh tuber weight per plant of storage roots in sweetpotato Different lowercase letters indicate significant differences at the P < 0.05 level."

Fig.3

Effects of drought stress and re-watering at different stages on O2-. , H2O2, MDA and proline contents in sweetpotato roots “*”and“**”indicate significant or extremely significant differences at the P < 0.05 and P < 0.01 levels, respectively, the same below."

Fig.4

Effects of drought stress and re-watering at different stages on SOD, POD, CAT and APX activities in sweetpotato roots"

Fig.5

Effects of drought stress and re-watering at different stages on the contents of IAA, GA, JA and ABA in sweetpotato roots"

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